Human Molecular Biology Laboratory Manual

Human Molecular Biology Laboratory Manual

Author: Stefan Surzycki

Publisher: John Wiley & Sons

Published: 2008-04-15

Total Pages: 246

ISBN-13: 0470999241

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Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use


Molecular Biology Techniques

Molecular Biology Techniques

Author: Heather B. Miller

Publisher: Academic Press

Published: 2011-10-18

Total Pages: 227

ISBN-13: 0123855454

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions


Molecular Biology

Molecular Biology

Author: S. K. Gakhar

Publisher:

Published: 2013-09-30

Total Pages: 0

ISBN-13: 9789382332305

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The objective of this text is to train young teachers from colleges and research institutions so that they can advance their research in various fields of biology. It will also help students at BSc and MSc level to learn the techniques involved in molecular biology. The book contains four chapters providing step-by-step protocols. In addition, it has general instructions for safety procedures.


Human Stem Cell Technology and Biology

Human Stem Cell Technology and Biology

Author: Gary S. Stein

Publisher: John Wiley & Sons

Published: 2011-03-04

Total Pages: 439

ISBN-13: 0470889896

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Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology Perspectives written by the world leaders in the field Introductory chapters will provide background information The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.


Phage Display

Phage Display

Author: Carlos F. Barbas

Publisher: CSHL Press

Published: 2001

Total Pages: 724

ISBN-13: 0879697407

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Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.


Genetics of Complex Human Diseases

Genetics of Complex Human Diseases

Author: Ammar Al-Chalabi

Publisher:

Published: 2009

Total Pages: 0

ISBN-13: 9780879698829

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Many human diseases – including Alzheimer's disease, schizophrenia, cancer, and cardiovascular disease – have complex genetics that require sophisticated analysis. Genetics of Complex Human Diseases: A Laboratory Manual& brings together the tools that geneticists use to find disease genes with the genetic concepts and statistical theories that underpin these research approaches. Topics covered include basic genetics and Mendelian inheritance, statistical methods, genetic epidemiology, linkage studies, transmission disequilibrium test analysis, variance components analysis, genome-wide association studies, copy-number variation, methods for high-throughput genotyping, the complexity of RNA editing, and genetic computer programs. The book's chapters, written by leading investigators in the field, blend practical information and reviews of each topic, providing both the how and the why of complex disease analysis. Genetics of Complex Human Diseasesis an important guide for anyone with an interest in human genetics or who uses genetic techniques in the study of diseases with complex inheritance.


Early Development of Xenopus Laevis

Early Development of Xenopus Laevis

Author: Hazel L. Sive

Publisher: CSHL Press

Published: 2000

Total Pages: 370

ISBN-13: 9780879695040

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Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.


Lab Manual for Human Biology

Lab Manual for Human Biology

Author: Sylvia Mader

Publisher: McGraw-Hill Education

Published: 2011-01-10

Total Pages: 288

ISBN-13: 9780077348625

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Business Communication is the newest Business Communication textbook that was created with students and professors needs in mind. A unique approach to a hands-on course, written by the co-authors of Business Communication: Making Connections in a Digital World, 12/e, provides both student and instructor with all the tools needed to navigate through the complexity of the modern business communication environment.


Laboratory Manual of Genetics

Laboratory Manual of Genetics

Author: A. M. Winchester

Publisher: McGraw-Hill Science, Engineering & Mathematics

Published: 1995-12

Total Pages: 248

ISBN-13:

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Give your students the opportunity to apply the scientific method to "real" -not simulated- lab investigations in both classical and molecular genetics. It is appropriate for a range of genetics and molecular biology laboratory courses because it incorporates material spanning the areas of basic genetics, molecular genetics, and human genetics. Since the first edition, "Laboratory Manual of Genetics has been carefully constructed to be student-oriented.


CRISPR-Cas

CRISPR-Cas

Author: Jennifer A. Doudna

Publisher:

Published: 2016

Total Pages: 0

ISBN-13: 9781621821304

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CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.