Histochemical Techniques
Histochemical Techniques

Author: J. D. Bancroft

Publisher: Butterworth-Heinemann

Published: 2013-10-22

Total Pages: 363

ISBN-13: 1483164713

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Histochemical Techniques, 2nd Edition deals with different methods of tissue processing and their application to histochemical procedures, including freeze drying and freeze substitution. Topics covered include fixation, production of paraffin and frozen sections, and principles of enzyme histochemistry. The use of histochemical methods to demonstrate carbohydrates, proteins, amino acids, lipids, nucleic acids, pigments, and various enzymes is also discussed. This book is comprised of 20 chapters and begins with an overview of the problems and values of histochemistry. The discussion then turns to carbohydrates, along with concepts and methods in carbohydrate histochemistry such as blocking and enzyme methods. Other chapters focus on amyloids, pigments, minerals, phosphatases, and esterases. The principles of enzyme histochemistry are also considered, with emphasis on preservation and factors affecting enzyme activity. The final chapter is devoted to ultra-histochemistry, the application of histochemistry to electron microscopy. This monograph will be of interest to histochemists, histopathologists, and technologists involved in histochemical work.

Histological & Histochemical Methods
Histological & Histochemical Methods

Author: John Alan Kiernan

Publisher: Pergamon

Published: 1990

Total Pages: 454

ISBN-13:

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Revised and updated edition (1st was 1981) of a textbook on chemical and physical principles of fixation, staining and histochemistry. For students i all biological subjects using histological techniques, as well as researcher and medical laboratory technologists. Annotation copyright Book News, Inc

Handbook of Histopathological and Histochemical Techniques
Handbook of Histopathological and Histochemical Techniques

Author: C. F. A. Culling

Publisher: Butterworth-Heinemann

Published: 2013-10-22

Total Pages: 727

ISBN-13: 1483164799

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Handbook of Histopathological and Histochemical Techniques (Including Museum Techniques), Third Edition is a reference guide on techniques used in histology and histochemistry, including museum techniques. This book is comprised of 36 chapters divided into six sections. After giving a brief description of the structure and contents of the cell, this book then turns to the various methods of examination of tissue cells and structures, including the smear method, vital staining, and electron microscopy. The next two sections deal with fixation, decalcification, processing, and section cutting, along with stains, impregnations, and mountants. An exhaustive survey of carbohydrate and protein histochemistry is included. Special procedures such as autoradiography, micro-incineration, and injection techniques are described. The remaining chapters focus on the preparation, mounting, and presentation of museum specimens. The book concludes with an analysis of the microscope. This monograph will be invaluable to all those with an interest in histology and histochemistry, including undergraduate students in the biological and health sciences, pathologists in both the hospital setting and experimental research, and technologists and workers in various research fields.

Bancroft's Theory and Practice of Histological Techniques E-Book
Bancroft's Theory and Practice of Histological Techniques E-Book

Author: Kim S Suvarna

Publisher: Elsevier Health Sciences

Published: 2012-10-01

Total Pages: 655

ISBN-13: 0702050326

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This is a brand new edition of the leading reference work on histological techniques. It is an essential and invaluable resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. This is a one stop reference book that the trainee histotechnologist can purchase at the beginning of his career and which will remain valuable to him as he increasingly gains experience in daily practice. Thoroughly revised and up-dated edition of the standard reference work in histotechnology that successfully integrates both theory and practice.Provides a single comprehensive resource on the tried and tested investigative techniques as well as coverage of the latest technical developments. Over 30 international expert contributors all of whom are involved in teaching, research and practice.Provides authoritative guidance on principles and practice of fixation and staining. Extensive use of summary tables, charts and boxes.Information is well set out and easy to retrieve. Six useful appendices included (SI units, solution preparation, specimen mounting, solubility). Provides practical information on measurements, preparation solutions that are used in daily laboratory practice. Color photomicrographs used extensively throughout. Better replicates the actual appearance of the specimen under the microscope. Brand new co-editors. New material on immunohistochemical and molecular diagnostic techniques.Enables user to keep abreast of latest advances in the field.

Histochemical and Cytochemical Methods of Visualization
Histochemical and Cytochemical Methods of Visualization

Author: Jean-Marie Exbrayat

Publisher: CRC Press

Published: 2013-06-05

Total Pages: 339

ISBN-13: 1439822255

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Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualizatio

Histochemistry of Single Molecules
Histochemistry of Single Molecules

Author: Carlo Pellicciari

Publisher: Springer Nature

Published: 2022-09-24

Total Pages: 351

ISBN-13: 1071626752

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This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.

Enzyme Histochemistry
Enzyme Histochemistry

Author: Z. Lojda

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 350

ISBN-13: 3642672345

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During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.

组织学技术
组织学技术

Author:

Publisher:

Published: 2008

Total Pages: 725

ISBN-13: 9787811165777

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本书内容全面,涵盖了组织学技术的方方面面,既包括传统的常用技术,又介绍了近年来新发展的,有较高实用价值的新技术。各章节分别论述了光镜、组织固定、样本处理、冰冻切片、石蜡切片、酶组织学、免疫组化、免疫荧光、组织芯片、原位杂交、荧光原位杂交、激光显微解剖、电镜等技术;对于结缔组织、糖类、脂类、蛋白质、核酸、色素及矿物质、淀粉、神经内分泌颗粒、微体、骨等相关的组绢学技术也予专门章节讲述;另外还涉及了显微镜样品定量、实验室安全及管理,以及人体工程学等相关内容。

Indian Herbal Drug Microscopy
Indian Herbal Drug Microscopy

Author: Shailendra S. Gurav

Publisher: Springer Science & Business Media

Published: 2013-11-20

Total Pages: 220

ISBN-13: 1461495156

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Adulteration and misidentification of herbal drugs can cause serious health problems to consumers. The first step in quality control of medicinal plants is ensuring the authenticity of the desired species for intended use, with anatomical study playing a critical role in identifying and authenticating medicinal plants. A product of numerous years of experience and research, Indian Herbal Drug Microscopy is a vital resource for identifying and evaluating Indian medicinal plants. Comprised of four concise and comprehensive chapters, the book presents stepwise procedures for sectioning of plant material, histo-chemical staining techniques, and the anatomy of forty well-known and medicinally important plants, including Arjuna, Ashoka, Ashwagandha, Cinchona, Cinnamon, Ginger, Kurchi, Rauwolfia, Turmeric, Tulsi, and Vasaka. The book is also supplemented with color photographs and hand-drawn microscopic images. Written by authorities in the field, Indian Herbal Drug Microscopy is a valuable guide for herbal drug microscopy of Indian medicinal plants.