High Resolution Separation and Analysis of Biological Macromolecules
High Resolution Separation and Analysis of Biological Macromolecules

Author: Barry L. Karger

Publisher: Elsevier

Published: 1996

Total Pages: 664

ISBN-13: 9780121821715

DOWNLOAD EBOOK

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features* Liquid chromatography* Electrophoresis* Mass spectrometry.

Biothermodynamics Part A
Biothermodynamics Part A

Author: Michael L. Johnson

Publisher: Academic Press

Published: 2009-03-14

Total Pages: 519

ISBN-13: 0080923402

DOWNLOAD EBOOK

In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. The use of thermodynamics in biological research is used as an "energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance? As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well.

Non-Natural Amino Acids
Non-Natural Amino Acids

Author:

Publisher: Academic Press

Published: 2009-07-24

Total Pages: 334

ISBN-13: 0080921639

DOWNLOAD EBOOK

By combining the tools of organic chemistry with those of physical biochemistry and cell biology, Non-Natural Amino Acids aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. - Demonstrates how the tools and principles of chemistry combined with the molecules and processes of living cells can be combined to create molecules with new properties and functions found neither in nature nor in the test tube - Presents new insights into the molecular mechanisms of complex biological and chemical systems that can be gained by studying the structure and function of non-natural molecules - Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

Constitutive Activity in Receptors and Other Proteins, Part A
Constitutive Activity in Receptors and Other Proteins, Part A

Author:

Publisher: Academic Press

Published: 2010-11-26

Total Pages: 821

ISBN-13: 0123812992

DOWNLOAD EBOOK

This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume. - Expert authors who are leaders in the field - Extensively referenced and useful figures and tables - Provides hints and tricks to facilitate reproduction of methods

The Unfolded Protein Response and Cellular Stress, Part C
The Unfolded Protein Response and Cellular Stress, Part C

Author:

Publisher: Academic Press

Published: 2011-02-14

Total Pages: 465

ISBN-13: 0123859298

DOWNLOAD EBOOK

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights - This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation - The authors explain how these methods are able to provide important biological insights

The Unfolded Protein Response and Cellular Stress, Part A
The Unfolded Protein Response and Cellular Stress, Part A

Author: P. Michael Conn

Publisher: Academic Press

Published: 2011-01-22

Total Pages: 427

ISBN-13: 0123851173

DOWNLOAD EBOOK

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. - This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation - The authors explain how these methods are able to provide important biological insights

Constitutive Activity in Receptors and Other Proteins, Part B
Constitutive Activity in Receptors and Other Proteins, Part B

Author:

Publisher: Academic Press

Published: 2010-11-24

Total Pages: 721

ISBN-13: 0123812976

DOWNLOAD EBOOK

This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume. - Expert authors who are leaders in the field - Extensively referenced and useful figures and tables - Provides hints and tricks to facilitate reproduction of methods

Cryo-EM Part A: Sample Preparation and Data Collection
Cryo-EM Part A: Sample Preparation and Data Collection

Author:

Publisher: Academic Press

Published: 2010-09-30

Total Pages: 466

ISBN-13: 0080956955

DOWNLOAD EBOOK

Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide

Small GTPases in Disease, Part A
Small GTPases in Disease, Part A

Author:

Publisher: Academic Press

Published: 2011-09-02

Total Pages: 512

ISBN-13: 0080877605

DOWNLOAD EBOOK

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect — full-text online of volumes 1 onwards. For more information about the Elsevier Book Series on ScienceDirect Program, please visit: http://www.info.sciencedirect.com/bookseries/ This volume is the first of two planned volumes on the topic of small GTPases and their role in disease.

Small GTPases in Disease, Part B
Small GTPases in Disease, Part B

Author:

Publisher: Academic Press

Published: 2008-04-01

Total Pages: 610

ISBN-13: 0080570070

DOWNLOAD EBOOK

The critically acclaimed laboratory standard for more than 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect — full-text online of volume 1 onward.