In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.
This book gives a practical introduction to one of the more popular separation techniques. Readers will learn to perform separations and will develop the ability to make an educated guess as to what the conditions will be to separate a new mixture of compounds. The authors provide classes of compound and background theory that quickly develop the skills of the student learning thin layer chromatography. Chapter coverage includes stationary phase, mobile phase, practical techniques, applications, recent developments, and advantages and disadvantages of thin layer chromatography. It also includes a bibliography of texts providing additional separations for further study.· Stationary Phase· Mobile Phase· Sample· Practical Techniques· Applications· Recent Developments· Advantages and Disadvantages of Tlc· Self Assessment Questions and Responses · Units of Measurement
Instrumental Thin-Layer Chromatography, Second Edition offers a comprehensive source of authoritative information on all aspects of instrumental thin-layer chromatography. The use of short, topic-focused chapters facilitates identifying information of immediate interest for familiar or emerging uses of thin-layer chromatography. The book gives those working in both academia and industry the opportunity to learn, refresh, or deepen their understanding of fundamental and instrumental aspects of thin-layer chromatography, as well as the tools to interpret and manage chromatographic data. The book serves as a practical consolidated guide to the selection of separation conditions and the use of auxiliary techniques. This fully updated new edition restores the contemporary character of the book for those involved in advancing the technology, analyzing data produced, or applying the technique to new application areas. Some chapters have been consolidated to make room for topics not covered in the first edition, reflecting general changes in the field of thin-layer chromatography, especially in effects-directed detection, convenient interfaces for advanced spectroscopic detection, and greater automation possibilities. This book is a valuable reference for anyone who needs to acquire fundamental and practical information to facilitate progress in research and management functions utilizing information acquired by thin-layer chromatography. Features individual chapters written by recognized authoritative and visionary experts in the field Provides an overview and focused treatment of a single topic Provides tables and diagrams with commonly used data to facilitate practical work, comparison of results, and decision-making Places modern developments in the research literature into a general context not always apparent to inexperienced users of the technique Offers comprehensive updates to all chapters Includes new chapters on instrument platforms, effects-directed detection, data analysis tools, small-scale and microfluidic planar separation systems, and applications to the separation of amino acids and peptides, the analysis of saccharides and lipids, and forensic analysis
Written by over 40 internationally acclaimed authorities on thin-layer chromatography (TLC), this comprehensive Second Edition presents the latest techniques, instrumentation, and applications of overpressurized, rotational, and high-performance quantitative TLC. Offering a systematic approach to TLC, the Handbook of Thin-Layer Chromatography, Second Edition contains new, practical information on the detection, identification, and documentation of chromatograph zones ... optical quantitation ... flame ionization detection ... automation and robotics ... nucleic acid derivatives ... and more.
In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.
Thin-Layer Chromatography (TLC) is a modern, reliable tool that complements other chromatographic techniques. This book provides a practical guide to the basic principles, procedures and pitfalls on the practical application of TLC. Thin Layer Chromatography: A Modern Practical Approach offers a sequence of chapters following the steps of the technique as the chromatographer would follow them. The chapters provide a choice of sorbent best suited to the separation intended, followed by pre-treatment required for the sample, applying the sample to the sorbent layer, development procedure, visualisation and detection, and finally quantification. Imaging and hyphenation techniques are described. The reasons why recommendations are made for specific and more general methods are covered. The book also provides an overview of some recent developments in the field.
A practical how-to guide to all the basic techniques needed to practice thin layer chromatography in biochemical/pharmaceutical research and quality control. This updated edition presents the most current techniques as well as the hows and whys of TLC. Provides step-by-step methods for performing the separations as well as doing related tasks, such as applying the sample, selecting the mobile phase, and quantitation. Includes a special chapter on how to select solvents for the development of a chromatogram to separate specific individual components of a mixture.
In the study and conservation of art and artifacts, natural organic materials are frequently encountered in components such as coatings, binders, and adhesives. The identification of these materials is often crucial to the attempt to characterize the technologies employed by artists or craftspeople, understand the processes and causes of deterioration, and plan appropriate conservation treatments. Yet the limited resources of many conservation laboratories put many analysis techniques beyond their reach. Thin-layer chromatography can help fill this gap. The volume consists of a handbook, protocols, and guide to reference materials. The handbook serves as a primer for the basic application of thin-layer chromatography to the analysis of binding media, adhesives, and coatings found on cultural objects; the protocols provide step-by-step instructions for the laboratory procedures involved in typical analyses; and the guide to reference materials aids in the understanding of the types of materials and documentation needed for accurate analyses by thin-layer chromatography.
Thin-layer chromatography has become so widely known in the space of a few years that it has proved necessary to gather into book form and thus make generally accessible the experimental material previously only available in isolated publications. As thin-layer chromatography can be used both for organic and inorganic matter as well as on quantities ranging from the nanogram to the microgram, it is impossible for anyone individual to possess sufficient laboratory experience or overall knowledge to produce a practical handbook that will be of real assistance to be ginner and specialist alike. For this reason, an international group was formed, who made it their task to produce the best possible treatise. In view of the present stage of development reached by thin-Iaycr chromatography, it seems specially apt that the authors should include yet unpublished work of their own. As thin-layer chromatography is used in many different fields in natural science and medicine, the kind of brief description of materials intelligible only to the expert has been avoided. The short guides to the chemical properties of the groups to be separated, their names, and relevant bibliographic details should facilitate introductory studies arid make possible a close acquaintance with the material in hand. It also seemed advisable to give brief details of the analytical classification of material, which is so often necessary. Although the classification used may appear unusual, it is in fact pre-eminently suitable to thin-layer chromatography.
