Genetic Manipulation of the Early Mammalian Embryo
Author: Frank Costantini
Publisher:
Published: 1985
Total Pages: 312
ISBN-13:
DOWNLOAD EBOOKPosts
Examining the State of the Science of Mammalian Embryo Model Systems
Author: National Academies of Sciences, Engineering, and Medicine
Publisher: National Academies Press
Published: 2020-08-31
Total Pages: 155
ISBN-13: 0309676681
DOWNLOAD EBOOKBecause of the recent advances in embryo modeling techniques, and at the request of the Office of Science Policy in the Office of the Director at the National Institutes of Health, the National Academies of Sciences, Engineering, hosted a 1-day public workshop that would explore the state of the science of mammalian embryo model systems. The workshop, which took place on January 17, 2020, featured a combination of presentations, panels, and general discussions, during which panelists and participants offered a broad range of perspectives. Participants considered whether embryo model systems - especially those that use nonhuman primate cells - can be used to predict the function of systems made with human cells. Presentations provided an overview of the current state of the science of in vitro development of human trophoblast. This publication summarizes the presentation and discussion of the workshop.
Manipulation of Mammalian Development
Author: Ralph B.L. Gwatkin
Publisher: Springer
Published: 1986-09-30
Total Pages: 416
ISBN-13:
DOWNLOAD EBOOKV.4 - Manipulation of mammalian development.
Manipulating the Mouse Embryo
Author: Richard Behringer
Publisher:
Published: 2014
Total Pages: 0
ISBN-13: 9781936113019
DOWNLOAD EBOOKEnthält: Genetics and embryology of the mouse: past, present, and future. Summary of mouse development. A mouse colony for the production of transgenic and chimeric animals. Recovery and in vitro culture of preimplantation embryos. Isolation, culture, and manipulation of postimplantation embryos. Surgical procedures. Production of transgenic mice by pronuclear microinjection. Embryo-derived stem cell lines. Germ line-competent stem cells derived from adult mice. Vector designs for pluripotent stem cell-based transgenesis and genome alterations. Introduction of foreign DNA into embryonic stem cells. Production of chimeras. Genotyping. Parthenogenesis, pronuclear transfer, and mouse cloning. Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection. Cryopreservation, rederivation, and transport of mouse strains. Techniques for visualizing gene products, cells, tissues, and organ systems. Setting up a micromanipulation lab.
Animal Applications of Research in Mammalian Development
Author: Roger A. Pedersen
Publisher: Banbury Report
Published: 1991
Total Pages: 360
ISBN-13:
DOWNLOAD EBOOKRecent progress in cell and molecular biology has profound implications for understanding the developmental biology of mammalian embryos. The unifying theme of this volume is a cellular and molecular approach to gametogenesis, embryogenesis, and maternal-fetal interactions in the developmental biology of agriculturally significant mammals. Great progress has been achieved in our understanding of preimplantation mammalian embryogenesis in laboratory species, particularly the mouse, through application of cell and embryo culture methods, cell biological techniques, and, more recently, recombinant DNA technology. A major remaining opportunity is the application of these techniques to problems in domestic species, such as improved methods for in vitro growth and maturation of oocytes, egg and embryo cryopreservation, and genetic manipulation to enhance productivity and herd quality. The authors review current knowledge in key areas, emphasizing new approaches to unsolved problems in the developmental biology of domestic species.
Mammalian Development
Author: Patrick P. L. Tam
Publisher:
Published: 2013
Total Pages: 0
ISBN-13: 9781936113248
DOWNLOAD EBOOK"A subject collection from Cold Spring Harbor perspectives in biology."
Manipulating the Mouse Embryo
Author: Brigid Hogan
Publisher:
Published: 1994
Total Pages: 0
ISBN-13: 9780879693848
DOWNLOAD EBOOKA comprehensive source of technical guidance for experienced investigators and essential resource for newcomers to mammalian genetics and embryology. This edition (1st ed., 1986) is revised and expanded to incorporate improvements to existing methods as well as new technologies. It contains new sect
Safety of Genetically Engineered Foods
Author: National Research Council
Publisher: National Academies Press
Published: 2004-07-08
Total Pages: 254
ISBN-13: 0309166152
DOWNLOAD EBOOKAssists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
A Laboratory Guide to the Mammalian Embryo
Author: David K. Gardner
Publisher: Oxford University Press
Published: 2004-01-08
Total Pages: 434
ISBN-13: 9780195142266
DOWNLOAD EBOOKNever before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.
Stem Cells and the Future of Regenerative Medicine
Author: Institute of Medicine
Publisher: National Academies Press
Published: 2002-01-25
Total Pages: 112
ISBN-13: 0309170427
DOWNLOAD EBOOKRecent scientific breakthroughs, celebrity patient advocates, and conflicting religious beliefs have come together to bring the state of stem cell researchâ€"specifically embryonic stem cell researchâ€"into the political crosshairs. President Bush's watershed policy statement allows federal funding for embryonic stem cell research but only on a limited number of stem cell lines. Millions of Americans could be affected by the continuing political debate among policymakers and the public. Stem Cells and the Future of Regenerative Medicine provides a deeper exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of undifferentiated human cells. In terms accessible to lay readers, the book summarizes what we know about adult and embryonic stem cells and discusses how to go about the transition from mouse studies to research that has therapeutic implications for people. Perhaps most important, Stem Cells and the Future of Regenerative Medicine also provides an overview of the moral and ethical problems that arise from the use of embryonic stem cells. This timely book compares the impact of public and private research funding and discusses approaches to appropriate research oversight. Based on the insights of leading scientists, ethicists, and other authorities, the book offers authoritative recommendations regarding the use of existing stem cell lines versus new lines in research, the important role of the federal government in this field of research, and other fundamental issues.
