Gene Cloning and DNA Analysis

Gene Cloning and DNA Analysis

Author: T. A. Brown

Publisher: John Wiley & Sons

Published: 2016-01-19

Total Pages: 374

ISBN-13: 1119072565

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Known world-wide as the standard introductory text to this important and exciting area, the seventh edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the chapters on DNA sequencing and genome studies have been rewritten to reflect the continuing rapid developments in this area of DNA analysis: In depth description of the next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes New material on the use of ChiP-seq to locate protein-binding sites Extended coverage of the strategies used to assemble genome sequences Description of how the Neanderthal genome has been sequenced and what that sequence tells us about interbreeding between Neanderthals and Homo sapiens Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves.


Gene Cloning and DNA Analysis

Gene Cloning and DNA Analysis

Author: T. A. Brown

Publisher: John Wiley & Sons

Published: 2013-04-25

Total Pages: 337

ISBN-13: 1118685288

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Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology. Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. "... the book content is elegantly illustrated and well organized in clear-cut chapters and subsections... there is a Further Reading section after each chapter that contains several key references... What is extremely useful, almost every reference is furnished with the short but distinct author's remark." –Journal of Heredity, 2007 (on the previous edition)


Gene Cloning

Gene Cloning

Author: Terence A. Brown

Publisher: Nelson Thornes

Published: 1998

Total Pages: 0

ISBN-13: 9780748740703

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Gene Cloning provides a basic introduction for students and researchers who have no previous experience of experiments with DNA, and assumes very little prior knowledge on the part of the reader. A three part structure addresses the basic principles of gene cloning, the application of cloning in gene analysis, and the role of gene cloning in research and biotechnology. The book is written in clear, jargon-free language, and is extensively illustrated with two-color line drawings.


Gene Cloning and DNA Analysis

Gene Cloning and DNA Analysis

Author: Terence A. Brown

Publisher:

Published: 2006

Total Pages: 386

ISBN-13:

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"Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students, including students of genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject."--Jacket.


Gene Cloning and DNA Analysis

Gene Cloning and DNA Analysis

Author: Terence A. Brown

Publisher:

Published: 2008

Total Pages: 386

ISBN-13:

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"Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students, including students of genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject."--Jacket.


Gene Cloning and DNA Analysis

Gene Cloning and DNA Analysis

Author: George Coppola

Publisher:

Published: 2019-06-11

Total Pages:

ISBN-13: 9781645320906

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Gene cloning is a common practice in molecular biology labs that is used by researchers to create copies of a particular gene for downstream applications, such as sequencing, mutagenesis, genotyping or heterologous expression of a protein. A fragment of DNA, containing the gene to be cloned, is inserted into a suitable vector, to produce a recombinant DNA molecule. The vector acts as a vehicle that transports the gene into a host cell usually a bacterium, although other types of living cell can be used. Within the host cell the vector multiplies, producing numerous identical copies not only of itself but also of the gene that it carries. When the host cell divides, copies of the recombinant DNA molecule are passed to the progeny and further vector replication takes place. After a large number of cell divisions, a colony, or clone, of identical host cells is produced. Each cell in the clone contains one or more copies of the recombinant DNA molecule; the gene carried by the recombinant molecule is now said to be cloned. This book discusses the basic principles of gene cloning and DNA analysis, and their applications in research and biotechnology.


DNA Cloning: A Hands-on Approach

DNA Cloning: A Hands-on Approach

Author: Seok-Yong Choi

Publisher: Springer

Published: 2019-04-17

Total Pages: 131

ISBN-13: 9402416625

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This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.


An Introduction to Genetic Engineering

An Introduction to Genetic Engineering

Author: Desmond S. T. Nicholl

Publisher: Cambridge University Press

Published: 2002-02-07

Total Pages: 308

ISBN-13: 9780521004718

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The author presents a basic introduction to the world of genetic engineering. Copyright © Libri GmbH. All rights reserved.


Gene Cloning

Gene Cloning

Author: Julia Lodge

Publisher: Garland Science

Published: 2007-01-24

Total Pages: 612

ISBN-13: 1135728925

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The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions of gene regulation and function made through bioinformatics. Applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture, are also covered in the book. Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource to both undergraduates and lecturers of biological and biomedical science courses.


Concepts of Biology

Concepts of Biology

Author: Samantha Fowler

Publisher:

Published: 2018-01-07

Total Pages: 618

ISBN-13: 9789888407453

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Concepts of Biology is designed for the single-semester introduction to biology course for non-science majors, which for many students is their only college-level science course. As such, this course represents an important opportunity for students to develop the necessary knowledge, tools, and skills to make informed decisions as they continue with their lives. Rather than being mired down with facts and vocabulary, the typical non-science major student needs information presented in a way that is easy to read and understand. Even more importantly, the content should be meaningful. Students do much better when they understand why biology is relevant to their everyday lives. For these reasons, Concepts of Biology is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand.We also strive to show the interconnectedness of topics within this extremely broad discipline. In order to meet the needs of today's instructors and students, we maintain the overall organization and coverage found in most syllabi for this course. A strength of Concepts of Biology is that instructors can customize the book, adapting it to the approach that works best in their classroom. Concepts of Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand--and apply--key concepts.