Molecular Biology Techniques

Molecular Biology Techniques

Author: Heather B. Miller

Publisher: Academic Press

Published: 2011-10-18

Total Pages: 227

ISBN-13: 0123855454

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions


Invertebrate Zoology

Invertebrate Zoology

Author: Robert L. Wallace

Publisher: Benjamin Cummings

Published: 1997

Total Pages: 356

ISBN-13:

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Appropriate for a laboratory course in invertebrate zoology. Invertebrate Zoology continues to be the most current, up-to-date manual available. The popular phylum- by-phylum approach has been retained, providing a solid conceptual framework for advanced work in behavior, ecology, physiology, and related subjects. Numerous exercises for studying the structure and function of invertebrates are used. To complete each exercise, students must make observations, conduct investigations, and ask and answer questions all of which helps them gain a comprehensive understanding of invertebrates.


Investigating Biology Laboratory Manual

Investigating Biology Laboratory Manual

Author: Judith Giles Morgan

Publisher: Benjamin-Cummings Publishing Company

Published: 2010

Total Pages: 0

ISBN-13: 9780321668219

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With its distinctive investigative approach to learning, this best-selling laboratory manual encourages you to participate in the process of science and develop creative and critical reasoning skills. You are invited to pose hypotheses, make predictions, conduct open-ended experiments, collect data, and apply the results to new problems. The Seventh Edition emphasizes connections to recurring themes in biology, including structure and function, unity and diversity, and the overarching theme of evolution. Select tables from the lab manual are provided in Excel® format in MasteringBiology® at www.masteringbiology.com, allowing you to record data directly on their computer, process data using statistical tests, create graphs, and be prepared to communicate your results in class discussions or reports.


The Dissection of Vertebrates

The Dissection of Vertebrates

Author: Gerardo De Iuliis

Publisher: Elsevier

Published: 2006-08-03

Total Pages: 299

ISBN-13: 0080477356

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The Dissection of Vertebrates covers several vertebrates commonly used in providing a transitional sequence in morphology. With illustrations on seven vertebrates – lamprey, shark, perch, mudpuppy, frog, cat, pigeon – this is the first book of its kind to include high-quality, digitally rendered illustrations. This book received the Award of Excellence in an Illustrated Medical Book from the Association of Medical Illustrators. It is organized by individual organism to facilitate classroom presentation. This illustrated, full-color primary dissection manual is ideal for use by students or practitioners working with vertebrate anatomy. This book is also recommended for researchers in vertebrate and functional morphology and comparative anatomy. The result of this exceptional work offers the most comprehensive treatment than has ever before been available. - Received the Award of Excellence in an Illustrated Medical Book from the Association of Medical Illustrators - Expertly rendered award-winning illustrations accompany the detailed, clear dissection direction - Organized by individual organism to facilitate classroom presentation - Offers coverage of a wide range of vertebrates - Full-color, strong pedagogical aids in a convenient lay-flat presentation


Plant Tissue Culture

Plant Tissue Culture

Author: Robert H. Smith

Publisher: Academic Press

Published: 2012-12-02

Total Pages: 190

ISBN-13: 0323160476

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Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.


Forensic Science Laboratory Manual and Workbook

Forensic Science Laboratory Manual and Workbook

Author: Thomas Kubic

Publisher: CRC Press

Published: 2018-07-31

Total Pages: 386

ISBN-13: 1420087215

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A laboratory companion to Forensic Science: An Introduction to Scientific and Investigative Techniques and other undergraduate texts, Forensic Science Laboratory Manual and Workbook, Third Edition provides a plethora of basic, hands-on experiments that can be completed with inexpensive and accessible instrumentation, making this an ideal workbook f


Laboratory Biosafety Manual

Laboratory Biosafety Manual

Author: World Health Organization

Publisher: World Health Organization

Published: 2004-12-28

Total Pages: 196

ISBN-13: 9789241546508

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This is the third edition of this manual which contains updated practical guidance on biosafety techniques in laboratories at all levels. It is organised into nine sections and issues covered include: microbiological risk assessment; lab design and facilities; biosecurity concepts; safety equipment; contingency planning; disinfection and sterilisation; the transport of infectious substances; biosafety and the safe use of recombinant DNA technology; chemical, fire and electrical safety aspects; safety organisation and training programmes; and the safety checklist.