Biocatalyst Immobilization
Biocatalyst Immobilization

Author: Maria Lujan Ferreira

Publisher: Academic Press

Published: 2022-11-12

Total Pages: 460

ISBN-13: 0323913776

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Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes. Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends. Provides a thorough overview of biocatalyst and enzyme immobilization for research and practical application Presents methods based content that instructs in enzyme immobilization pretreatment, enzyme supports, post immobilization treatments, measuring enzyme immobilization efficiency, quantification of reaction products, and whole cell immobilization Features chapter contributions from international leaders in the field

Immobilized Enzyme Technology
Immobilized Enzyme Technology

Author: H. Weetall

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 317

ISBN-13: 1461587352

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On November 5-8, 1974, a Seminar on Research and De velopment of Immobilized Enzymes was held in Tokyo, Japan. The seminar was part of the United States-Japan Cooperative Science Program sponsored jointly by the National Science Foundation and the Japan Society for the Promotion of Sci ence. The purpose of the seminar was to promote the scienti fic exchange of ideas and scien~ific results of a practical nature, as well as academic advances made in both countries through discussion and exchange of ideas. The areas chosen for discussion included: fundamental research in immobilized enzymes, new techniques of enzyme purification, comparative studies on immobilization techni ques and the relative merits, chemical engineering aspects of the technology, industrial applications and reactor de sign. The discussions and exchange of ideas which took place at the seminar should promote new research and development which we hope will lead to new important advances in enzyme technology. These proceedings represent the summation of the work presented and discussed at the seminar. The editors hope the reader will find them interesting and informative. Howard H. Weeta11 Shuichi Suzuki March, 1975 v Contents Immobilization of Coenzyme B6 and Several B6 Enzymes: Application to Assay or Production of SOU,I;! Amino Acids and to Structure-Function Inter relationship Studies of B6 1 Enzymes • • • • • • • Saburo Fukui and Sei-ichiro Ikeda Enzyme Engineering Case Study: Immobilized Lactase • • • • • 17 James R. Ford and Wayne H. Pitcher, Jr.

Immobilized Enzymes
Immobilized Enzymes

Author:

Publisher: Academic Press

Published: 1976

Total Pages: 1030

ISBN-13: 9780121819446

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The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

Carrier-bound Immobilized Enzymes
Carrier-bound Immobilized Enzymes

Author: Linqiu Cao

Publisher: John Wiley & Sons

Published: 2006-05-12

Total Pages: 578

ISBN-13: 3527607080

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The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, such as adsorption-based and covalent immobilization, as well as enzyme entrapment and encapsulation. Throughout, a careful review of materials and techniques for the generation of functional immobilized enzymes benefits both developers and users of carrier-bound enzymes. A must for biotechnologists, biochemists and preparative chemists using enzymes in their daily work.

Immobilization of Enzymes and Cells
Immobilization of Enzymes and Cells

Author: Jose M. Guisan

Publisher: Humana

Published: 2021-01-29

Total Pages: 495

ISBN-13: 9781071602171

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This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.