Fixation, Dehydration and Embedding of Biological Specimens
Author: Audrey M. Glauert
Publisher:
Published: 1975
Total Pages: 353
ISBN-13: 9780720442502
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Biological Electron Microscopy
Author: Michael J. Dykstra
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 368
ISBN-13: 146840010X
DOWNLOAD EBOOKIn this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.
Biological Specimen Preparation for Transmission Electron Microscopy
Author: Audrey M. Glauert
Publisher: Princeton University Press
Published: 2014-07-14
Total Pages: 349
ISBN-13: 1400865026
DOWNLOAD EBOOKThis book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
Cryotechniques in Biological Electron Microscopy
Author: Rudolf A. Steinbrecht
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 308
ISBN-13: 3642728154
DOWNLOAD EBOOKTo preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.
Fixation, Dehydration and Embedding of Biological Specimens
Author: A.M. Glauert
Publisher: Elsevier Science
Published: 1987-09-15
Total Pages: 353
ISBN-13: 9780720442571
DOWNLOAD EBOOKScanning Electron Microscopy for the Life Sciences
Author: Heide Schatten
Publisher: Cambridge University Press
Published: 2013
Total Pages: 275
ISBN-13: 0521195993
DOWNLOAD EBOOKA guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.
Advanced Techniques in Biological Electron Microscopy
Author: J.K. Koehler
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 319
ISBN-13: 3642654924
DOWNLOAD EBOOKThe past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.
Fixation, Dehydration and Embedding of Biological Specimens
Author: Audrey M. Glauert
Publisher:
Published: 1975
Total Pages: 353
ISBN-13: 9780720442502
DOWNLOAD EBOOKFixation, dehydration and embedding of biological specimens / Audrey M. Glauert. Ultramicrotomy / Norma Reid.
Microscopy of the Heart
Author: Lars Kaestner
Publisher: Springer
Published: 2018-12-07
Total Pages: 135
ISBN-13: 3319953044
DOWNLOAD EBOOKThis book provides in depths information on different microscopy approaches and supplies the reader with methods how to untangle highly complex processes involved in physiological and pathophysiological cardiac signaling. Microscopy approaches have established themselves as the quasi gold standard that enables us to appreciate the underlying mechanisms of physiological and pathophysiological cardiac signaling. This book presents the most important microscopy techniques from the level of individual molecule e.g. Förster-Resonance Energy Transfer (FRET), up to cellular and tissue imaging, e.g. electron microscopy (TEM) or light sheet microscopy. The book is intended for graduate students and postdocs in cardiovascular research, imaging and cell biology, pre-clinical and clinical researchers in cardiovascular sciences as well as decision makers of the pharmaceutical industry.
Electron Microscopy
Author: John J. Bozzola
Publisher: Jones & Bartlett Learning
Published: 1999
Total Pages: 702
ISBN-13: 9780763701925
DOWNLOAD EBOOKNew edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR
