Enzyme-Mediated Immunoassay
Enzyme-Mediated Immunoassay

Author: H.M. Lenhoff

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 481

ISBN-13: 1468450123

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T. T. Ngo and H. M. Lenhoff Department of Developmental and Cell Biology University of California, Irvine, CA 92717 In 1959, Yalow and Berson used insulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma. Their method depends upon ~ competition between insulin labeled with radioactive iodine (II 1) and unlabeled insulin from plasma for a fixed and limited number of specific binding sites on the antibody to insulin. The amount of the labeled insulin bound to the antibody is inversely proportional to the amount of insulin in the plasma sample. Their method, which is so elegantly simple in concept, is made possible by the ability to detect with ease extremely low levels of radioactivity, and by the exquisite specificity of an antibody capable of specifically binding the analyte. Such a combination of sensitivity and specificity is the basis of this versatile analytical tool called radioimmunoassay (RIA). Twelve years later, Engvall and Perlmann (1971) and Van Weemen and Schuurs (1971) independently introduced the use of enzymes as another category of sensitive and even more versatile labels for use in immunoassays. Engvall and Perlmann (l971) coined the term ELISA, which stands for Enzyme Linked Immunosorbent Assay.

Enzyme-linked Immunosorbent Assay (ELISA)
Enzyme-linked Immunosorbent Assay (ELISA)

Author: Samira Hosseini

Publisher: Springer

Published: 2017-12-30

Total Pages: 124

ISBN-13: 981106766X

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This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.

Immunoassay
Immunoassay

Author: Eleftherios P. Diamandis

Publisher: Academic Press

Published: 1996-06-21

Total Pages: 613

ISBN-13: 0080534503

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Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians. - Presents up-to-date information - Provides extensive cross-referencing - Covers theory and practice in full detail - Written by leading authorities

Enzyme Immunoassays
Enzyme Immunoassays

Author: S.S. Deshpande

Publisher: Springer Science & Business Media

Published: 1996-06-30

Total Pages: 488

ISBN-13: 9780412056017

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This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoessay technology. Presenting both the basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market.

Gastrointestinal Diseases and Their Associated Infections
Gastrointestinal Diseases and Their Associated Infections

Author: Guy D. Eslick

Publisher: Elsevier Health Sciences

Published: 2019-01-17

Total Pages: 285

ISBN-13: 032354844X

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Many bacteria, viruses, protozoa, and fungi play key roles in the development of gastrointestinal diseases, and this practical reference brings you up to speed with this increasingly important area. Covering a broad range of GI diseases and cancers, this resource provides an expert overview of the field, ideal for all gastroenterologists and infectious disease physicians. - Covers infections associated with gastroesophageal reflux disease and Barrett's esophagus, gallbladder disease, acute pancreatitis, small intestinal bacterial overgrowth, irritable bowel syndrome, inflammatory bowel disease, appendicitis, Whipple Disease, Crohn's Disease, and more. - Discusses esophageal cancer, gastric cancer, cholangiocarcinoma, hepatocellular carcinoma, and colorectal cancer. - Includes chapters on gut microbiome, fecal transplants, and the molecular pathgenesis of gastrointestinal infections. - Consolidates today's available information on this timely topic into a single convenient resource.

Practice and Theory of Enzyme Immunoassays
Practice and Theory of Enzyme Immunoassays

Author: P. Tijssen

Publisher: Elsevier

Published: 1985-08-01

Total Pages: 577

ISBN-13: 0080858848

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Enzyme immunoassays have developed into a powerful assay technology, transcending several discipline boundaries, extensively applied as a tool in fields other than enzymology and immunology. This volume reflects the rapid progress in the applications of this technique, providing a basic understanding of these techniques and a practical guideline for the choice and experimental detail.

Nonisotopic Immunoassay
Nonisotopic Immunoassay

Author: That T. Ngo

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 487

ISBN-13: 1468454668

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The basis of all immunoassays is the interaction of antibodies with antigens. The most widely used immunoassay technique is radioimmunoassay (RIA) which was first developed by Yalow and Berson in 1959. The principle of RIA is elegantly simple. It utilizes a competitve binding reaction between analytes and a radio-labeled analog of the analytes (the tracer) for anti-analyte antibodies. In addition to its exquisite specificity, extraordinary sensitivity, good accuracy and precision, ease and rapidity of assay and simplicity of assay development, the applicability of RIA to a wide variety of substances has made it one of the most powerful and versatile analytical methods of the 20th century and beyond. Millions of RIA's are being performed annually on clinical, biological and environmental samples in licensed laboratories. In order to expand the use of RIA beyond the confines of these laboratories to areas like physician's offices, patients' homes, economically less developed countries, agricultural fields, large scale and continuing screening tests for infectious diseases, it has become necessary to develop non-isotopic labels. Indeed the last fifteen years have seen the development of a great number of ingenious non-isotopic labels in immunoassay so that a whole new industry capitalizing on the potential market for non isotopic immunoassays has appeared. It is the purpose of this volume to present in depth, state-of-the-art reviews on techniques used in non-isotopic immunoassays. Topics covered include: (1) Enzyme-labeled immunoassay; (2) Luminescene immunoassay; (3) Immunoassay at liquid-solid interface; (4) Membrane immunoassay and (5) "Particle"-mediated immunoassay.

Biosensors Based on Sandwich Assays
Biosensors Based on Sandwich Assays

Author: Fan Xia

Publisher: Springer

Published: 2018-01-09

Total Pages: 220

ISBN-13: 9811078351

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This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.

Handbook of Immunoassay Technologies
Handbook of Immunoassay Technologies

Author: Sandeep K. Vashist

Publisher: Academic Press

Published: 2018-01-02

Total Pages: 498

ISBN-13: 012811794X

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Handbook of Immunoassay Technologies: Approaches, Performances, and Applications unravels the role of immunoassays in the biochemical sciences. During the last four decades, a wide range of immunoassays has been developed, ranging from the conventional enzyme-linked immunosorbent assays, to the smartphone-based point-of-care formats. The advances in rapid biochemical procedures, novel biosensing schemes, fully integrated lab-on-a-chip platforms, prolonged biomolecular storage strategies, device miniaturization and interfacing, and emerging smart system technologies equipped with personalized mobile healthcare tools are paving the way to next-generation immunoassays, and are all discussed in this comprehensive text. Immunoassays play a prominent role in clinical diagnostics as they are the eyes of healthcare professionals, helping them make informed clinical decisions via confirmed disease diagnosis, and thus enabling favorable health outcomes. The faster and reliable diagnosis of infections will further control their spread to uninfected persons. Similarly, immunoassays play a prominent role in veterinary diagnostics, food analysis, environmental monitoring, defense and security, and other bioanalytical settings. Therefore, they enable the detection of a plethora of analytes, which includes disease biomarkers, pathogens, drug impurities, environmental contaminants, allergens, food adulterants, drugs of abuse and various biomolecules. - Provides a valuable increase of understanding of cellular and biomedical functions - Gives the most updated resource in the field of immunoassays, providing the comprehensive details of various types of immunoassays that need to be performed in healthcare, and in industrial, environmental and other biochemical settings - Discusses all multifarious aspects of immunoassays - Describes the immunoassay formats, along with their principle of operation, characteristics, pros and cons, and potential biochemical and bioanalytical applications - Provides extensive knowledge and guided insights as detailed by experienced, renowned experts and key opinion makers in the field of immunoassays

Enzyme Immunoassay
Enzyme Immunoassay

Author: Edward T. Maggio

Publisher: CRC Press

Published: 2018-01-18

Total Pages: 305

ISBN-13: 1351080296

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The purpose of this book is to focus attention on some of these ideas and concepts. In doing so, it has captured a glimpse of the past and it attempts a projection of the future, but mostly it reveals an overview of the field as it exists as the present time. It aims to serve to spawn further growth in ideas and encourage applications to increasingly broader segments of both clinical and general analytical chemistry fields.