Enzyme Histochemistry
Enzyme Histochemistry

Author: Z. Lojda

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 350

ISBN-13: 3642672345

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During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.

Histochemistry
Histochemistry

Author: Richard W. Horobin

Publisher: Elsevier

Published: 2014-05-16

Total Pages: 324

ISBN-13: 1483164683

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Histochemistry: An Explanatory Outline of Histochemistry and Biophysical Staining describes the histochemical staining of cells and tissues as a major tool applied in biological and medical investigations, both in basic research and in practical applications such as clinical diagnosis. The book may be considered as a guide to understanding the scientific basis of staining procedures and alternate actions to take when common methods do not proceed as expected. The first chapter gives general theoretical ideas from which most part of the book is largely organized around. As the book considers the arts and crafts making up the practice of histochemistry and biological staining, emphasis is given to the common physicochemical aspects of the technically diverse methodologies involved. Hence, the author has drawn ideas and information from physicochemically and biochemically related fields, such as chromatography, pharmacology, photography, tanning, and textile dyeing. The bulk of the book is structured around groups of practical procedures, such as fixation, staining with dyestuffs, metal impregnation, and selective extraction as a test of significance. Lastly, general essays on the usefulness of staining theories and on the problems arising from reagent impurities are given as examples. The text is suitable for students and researchers in the fields of physiology, biochemistry, and pharmacology. Clinical laboratory scientists and those involved in the life sciences and biotechnology will benefit from reading the book.

Current Catalog
Current Catalog

Author: National Library of Medicine (U.S.)

Publisher:

Published:

Total Pages: 1378

ISBN-13:

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First multi-year cumulation covers six years: 1965-70.

Cytology and Cell Physiology
Cytology and Cell Physiology

Author: Geoffrey Bourne

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 799

ISBN-13: 0323162010

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Cytology and Cell Physiology, Third Edition focuses on cell cytology and physiology as well as recent advances in the techniques in studying cells, including microscopy. It also describes cell membranes, surface, and physiology; cytoplasmic constituents; nucleus and nucleocytoplasmic reactions; enzyme histochemistry and cytochemistry; viruses within cells; and morphology of the cancer cell. Organized into 13 chapters, this edition begins with a historical overview of cytology, the template hypothesis of protein synthesis, and the respiratory function of mitochondria. It then discusses the subcellular components and their centrifugal isolation, some general principles of microscopy, selected physical and physicochemical methods, applications of enzyme histochemistry to electron microscopy, and structure and physical properties of the plasma membrane. The remaining chapters focus on the endoplasmic reticulum, the Golgi apparatus, the nucleus and its role in cell metabolism, RNA synthesis and movement, the behavior of viruses within cells, and pathological changes in cells. The book concludes with a chapter on the function and metabolism of cancer cells. This book is highly recommended to cytologists, investigators in the field of pathology, and graduate students in biology, biochemistry, physiology, and anatomy.

Manual of Histological Techniques
Manual of Histological Techniques

Author: Santosh Kumar Mondal

Publisher: JP Medical Ltd

Published: 2017-01-31

Total Pages: 208

ISBN-13: 9386261197

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In this manual, author has included the molecular techniques, immunohistochemistry, cell blocks, and immunofluorescence along with the conventional techniques. For students' easy understanding; many figures, charts, diagrams and tables have been included. At the same time, the volume of the book has been restricted; so that students do not become overburdened during preparation of examination. [Ed.].

Histological & Histochemical Methods
Histological & Histochemical Methods

Author: John Alan Kiernan

Publisher: Pergamon

Published: 1990

Total Pages: 454

ISBN-13:

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Revised and updated edition (1st was 1981) of a textbook on chemical and physical principles of fixation, staining and histochemistry. For students i all biological subjects using histological techniques, as well as researcher and medical laboratory technologists. Annotation copyright Book News, Inc

Advanced Techniques in Plant Sciences
Advanced Techniques in Plant Sciences

Author: Dr. D. A. Dhale

Publisher: Book Saga Publications

Published: 2023-05-28

Total Pages: 316

ISBN-13: 9395470429

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This book, ‘Advanced Techniques in Plant Sciences’ includes basic and advanced techniques like microscopy, polarizing microscopy, transmission and scanning electron microscopes, confocal microscopy, phase contrast microscopy, photomicrography, digital imaging, and microscopic measurements. The Spectroscopic techniques contain Colorimeter, Spectroscopic techniques, Ultraviolet-visible spectroscopy; Infrared spectroscopy, Nuclear magnetic resonance spectroscopy; Basic NMR techniques, NMR Imaging Principles, Electron Spin Resonance (ESR) Spectroscopy, Atomic Absorption Spectroscopy (AAS); Mass spectrometry, Flame photometer, etc. In histochemical studies, various methods include proteins, carbohydrates, lipids, and enzymes; how to prepare the macrolides; and the different methods of preparing the slides, which include Along with this, you will also study whole mounts. Also, about the sectioning of plant specimens, free hand, microtome, rotary microtome sectioning, types of microtome, and methods of serial sectioning. Maceration, Gel electrophoresis, PAGE, SDS-PAGE, Agarose gel electrophoresis; isoelectric focusing, and 2D electrophoresis Blotting Techniques: Principles and Techniques of Southern, Northern, and Western Blotting Techniques and Southwestern Blotting. Centrifugation techniques, Types of Centrifugation, Ultracentrifugation, Concept of Partition Coefficient; Chromatography, Techniques by chromatographic bed shape, Paper Chromatography, Double-way paper chromatography, Planar chromatography, Thin layer chromatography (TLC), High-performance liquid chromatography (HPLC)- Partition chromatography, Normal-phase chromatography, Displacement chromatography, Reversed-phase chromatography (RPC), Size-exclusion chromatography, Ion-exchange chromatography, Bioaffinity chromatography, Aqueous normal-phase chromatography, Isocratic flow, and gradient elution; High-Performance Thin-Layer Chromatography (HPTLC), Gas chromatography (GC), Flash column chromatography, Column chromatography, Ion chromatography, Gel-filtration chromatography or Size-exclusion chromatography, Pyrolysis gas chromatography, Countercurrent chromatography.

The Kidney Morphology, Biochemistry, Physiology
The Kidney Morphology, Biochemistry, Physiology

Author: Charles Rouiller

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 445

ISBN-13: 0323150071

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The Kidney: Morphology, Biochemistry, and Physiology, Volume IV covers the developments in the study of renal structure and function, particularly on the subcellular and molecular level. This book is composed of six chapters that consider the correlation of kidney's structure with function, as well as the participation of the kidney in metabolic systems and its relation to other organs. This text discusses topics such as experimental and renal transplantation, the structure and function of the juxtaglomerular apparatus, and the macula densa. Other chapters deal with the gaseous and substrate metabolism of the kidney and the role of this organ in the osmotic concentration and dilution of the urine. The remaining chapters explore hydrogen secretion, potassium and sodium excretion, and the correlation of the kidney with vitamins and parathyroid gland. This book is of value to morphologists, biochemists, physiologists, pathologists, pharmacologists, and clinicians.