Electrophoresis methods are simple and informative analytical methods, which are used in biochemistry and medicine. They are necessary for precise investigation of proteins and nucleic acids (DNA and RNA). Electrophoresis is practiced in chemical, biochemical, and clinical laboratories, as well as in forensic medicine laboratories. This book will discuss the fundamentals of electrophoresis methods at the graduate level.
Selected, peer reviewed papers from the 5th International Conference on Electrophoretic Deposition: Fundamentals and Applications, October 5-10, 2014, Hernstein, Austria
Fundamentals of Forensic Science, Third Edition, provides current case studies that reflect the ways professional forensic scientists work, not how forensic academicians teach. The book includes the binding principles of forensic science, including the relationships between people, places, and things as demonstrated by transferred evidence, the context of those people, places, and things, and the meaningfulness of the physical evidence discovered, along with its value in the justice system. Written by two of the leading experts in forensic science today, the book approaches the field from a truly unique and exciting perspective, giving readers a new understanding and appreciation for crime scenes as recent pieces of history, each with evidence that tells a story. - Straightforward organization that includes key terms, numerous feature boxes emphasizing online resources,historical events, and figures in forensic science - Compelling, actual cases are included at the start of each chapter to illustrate the principles being covered - Effective training, including end-of-chapter questions – paired with a clear writing style making this an invaluableresource for professors and students of forensic science - Over 250 vivid, color illustrations that diagram key concepts and depict evidence encountered in the field
This thoroughly revised edition of the book demonstrates principle and instrumentation of each technique routinely used in biotechnology. Like the previous edition, the second edition also follows non-mathematical approach. Three aspects of each technique including principle, methodology with knowledge of different parts of an instrument; and applications have now been discussed in the text. For the beginners, the book will help in building a strong foundation, starting from the preparation of solutions, extraction, separation and analysis of biomolecules to the characterisation by spectroscopic methods—the full gamut of biological analysis. NEW TO THE SECOND EDITION • Incorporates two new chapters on 'Radioisotope Tracer Techniques' and 'Basic Molecular Biology Techniques and Bioinformatics'. • Comprises a full chapter on 'Fermentation and Bioreactors' Design and Instrumentation' (the revised and updated version of Miscellaneous Methods of the previous edition). • Contains a number of pictorial illustrations, tables and worked-out examples to enhance students' understanding of the topics. • Includes chapter-end review questions. TARGET AUDIENCE • B.Sc./B.Tech (Biotechnology) • M.Sc./M.Tech (Biotechnology)
Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Fundamentals of Biochemical Pharmacology explains the molecular aspects of drugs and the changes in bio-chemical systems. The cellular movements that result from such changes are also evaluated. Biochemical lesion is extensively defined in the book. A discussion on electromagnetic radiation is also provided. A chapter of the book is devoted to the principles of electronic and nuclear magnetic resonance. The principles and applications of mass spectrometry and combined gas chromatography are then discussed. The scientific advances made with the use of immunological methods are the focus of a section of the book. Another section provides an introduction to the kinetic properties of reactions made by enzymes. The process called homogenization is clearly explained along with a discussion on the use of electron microscopy. Autoradiography shows the distribution of compounds at the subcellular level. The theoretical background of molecular spectroscopy is presented completely. The book is intended for chemists, biochemists, physicists, micro-biologists, zoologists, and botanists .
A condensed, easier-to-understand student version of the acclaimed Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 7th Edition uses a laboratory perspective in providing the clinical chemistry fundamentals you need to work in a real-world, clinical lab. Coverage ranges from laboratory principles to analytical techniques and instrumentation, analytes, pathophysiology, and more. New content keeps you current with the latest developments in molecular diagnostics. From highly respected clinical chemistry experts Carl Burtis and David Bruns, this textbook shows how to select and perform diagnostic lab tests, and accurately evaluate results. Authoritative, respected author team consists of two well-known experts in the clinical chemistry world. Coverage of analytical techniques and instrumentation includes optical techniques, electrochemistry, electrophoresis, chromatography, mass spectrometry, enzymology, immunochemical techniques, microchips, automation, and point of care testing. Learning objectives begin each chapter, providing measurable outcomes to achieve after completing the material. Key words are listed and defined at the beginning of each chapter, and bolded in the text. A glossary at the end of the book makes it quick and easy to look up definitions of key terms. More than 500 illustrations plus easy-to-read tables help you understand and remember key concepts. New chapters on molecular diagnostics include the principles of molecular biology, nucleic acid techniques and applications, and genomes and nucleic acid alterations, reflecting the changes in this rapidly evolving field. New content on clinical evaluation of methods, kidney function tests, and diabetes is added to this edition. NEW multiple-choice review questions at the end of each chapter allow you to measure your comprehension of the material. NEW case studies on the Evolve companion website use real-life scenarios to reinforce concepts.
"Electrophoresis in Practice" ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese.
