A Manual of Quantitative Immunoelectrophoresis
Author: Nils H. Axelsen
Publisher:
Published: 1977
Total Pages: 176
ISBN-13: 9780632004027
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Author: Nils H. Axelsen
Publisher:
Published: 1977
Total Pages: 176
ISBN-13: 9780632004027
DOWNLOAD EBOOKAuthor: Leo P. Cawley
Publisher:
Published: 1969
Total Pages: 0
ISBN-13: 9780316132305
DOWNLOAD EBOOKAuthor: A. Nowotny
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 328
ISBN-13: 3642673562
DOWNLOAD EBOOKAuthor: David F. Keren
Publisher: Butterworth-Heinemann
Published: 2013-10-22
Total Pages: 249
ISBN-13: 1483165264
DOWNLOAD EBOOKHigh-Resolution Electrophoresis and Immunofixation: Techniques and Interpretation acquaints the reader with the available methodologies and instrumentation for performing high-resolution electrophoresis and immunofixation. This text presents the use of procedures of high-resolution electrophoresis and immunofixation to facilitate the diagnosis of a variety of serum protein abnormalities. It also provides case examples to illustrate the uses of these procedures and their possible caveats. The book contains chapters devoted to the methods and rationale for high-resolution electrophoresis; interpretation of high-resolution electrophoresis patterns in serum, urine, and cerebrospinal fluid; the immunofixation technique; and strategies for diagnosing monoclonal gammopathies. Medical technologists, physicians, and pathologists will find the book invaluable.
Author: Michael J Dunn
Publisher: Elsevier
Published: 2014-05-12
Total Pages: 422
ISBN-13: 1483193543
DOWNLOAD EBOOKGel Electrophoresis of Proteins focuses on the techniques, methodologies, reactions, and approaches involved in gel electrophoresis of proteins. The selection first covers steady-state gel electrophoresis systems and one-dimensional PAA-gel electrophoretic techniques to separate functional and denatured proteins. Discussions focus on affinity electrophoresis, structure and physico-chemical properties of polyacrylamide gels, moving boundary electrophoresis, isotachophoresis, fundamental steady-state electrophoresis systems, and fundamental properties of steady-state electrophoresis systems. The text then reviews conventional isoelectric focusing and immobilized pH gradients and high resolution two-dimensional polyacrylamide-gel electrophoresis. Topics include production of narrow pH gradients, extended pH gradients, polymerization kinetics, IEF in agarose matrices, titration curves, two-dimensional electrophoresis under non-denaturing conditions, and IEF in polyacrylamide matrices. The book tackles quantifying patterns from two-dimensional PAGE, protein staining and detection methods, and immunoelectrophoretic methods. Concerns include post-electrophoretic organic protein stains, silver and enzyme stains, detection of radioactive proteins, and programming languages. The selection is highly recommended for researchers wanting to conduct studies on gel electrophoresis of proteins.
Author: Reiner Westermeier
Publisher: John Wiley & Sons
Published: 2016-05-16
Total Pages: 470
ISBN-13: 3527338802
DOWNLOAD EBOOK"Electrophoresis in Practice" ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese.
Author:
Publisher:
Published: 1973
Total Pages: 36
ISBN-13:
DOWNLOAD EBOOKAuthor: John M. Walker
Publisher: Springer
Published: 2007-10-02
Total Pages: 1099
ISBN-13: 1592591698
DOWNLOAD EBOOKThe Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Author:
Publisher:
Published: 1972
Total Pages: 220
ISBN-13:
DOWNLOAD EBOOKAuthor: Bonnie S. Dunbar
Publisher: Springer
Published: 1988-07-31
Total Pages: 398
ISBN-13: 9780306428395
DOWNLOAD EBOOKThis text is a summary of basic principles and techniques and is dedicated to all those students who have been told by their mentors, "Go forth and do two-dimensional gels and have the results on my desk tomorrow. " No attempt has been made in this text to provide exhaustive lists of references related to basic principles or techniques or to list every com pany or supplier involved in this area of research. Nevertheless, it is hoped that sufficient information is given to help a new investigator or student appreciate the complexities but develop sufficient expertise to carry out these techniques successfully. The discussions are designed to instill in basic science and clinical investigators of all levels of expertise an appreciation of the power of combining a variety of techniques as well as to provide basic insight into the theories, complexities, and problems frequently encountered with electrophoretic and immunochemical meth ods. Bonnie S. Dunbar Houston v Acknowledgments I wish to thank my students and staff for their patience and support throughout the preparation of this text. I would like to acknowledge my appreciation for my extensive discussions with Dr. David Sammons (Uni versity of Arizona) and to Dr. N. L. and Dr. N. G. Anderson and their colleagues (Argonne National Laboratory) for their invaluable advice and suggestions in this area over the years. I thank my research assistant, Ms.