This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.
As global criminology evolves, new questions arise. How can policymakers and law enforcement agencies leverage criminological insights to formulate effective crime prevention strategies? How do international collaborations in forensic science contribute to a more robust global criminal justice framework? Cases on Forensic and Criminological Science for Criminal Detection and Avoidance beckons readers into the fascinating world of forensic science and criminology. Distinguished authors span multi-level experience as practitioners, researchers, and academics, and they examine the multifaceted dimensions of criminal detection and avoidance. Contributors, hailing from globally recognized institutions such as the British Criminology Association, Chartered Society of Forensic Sciences, and American Criminology Association, navigate the complex intersections of forensic science and criminology, exploring the latest criminal detection and avoidance advancements. Moreover, the book examines the practical implications of research findings. This book is ideal for academics, practitioners, policymakers, students, and professionals in criminology, forensic science, and criminal justice seeking a comprehensive exploration of contemporary criminal detection and avoidance issues.
Medical Microbiology deals with the study of microorganisms that colonize or infect humans. It studies the pathogenesis and epidemiology of these microorganisms with practical applications in diagnosis, treatment and prevention of diseases (infectious diseases, infection-related cancer, dysbiosis related diseases, etc.,). In the past two decades, its expansion is essentially due to the great advances in related fields such as immunology, genetics, bioinformatics, science, technology, engineering and mathematics. New scientific knowledge and understanding make possible new applications. Nevertheless, it becomes a challenge due to lack of expertise to analyze fast-growing data and to translate them in clinical practice and medical education. The pace and sophistication of advances in all scientific disciplines applied to medical microbiology call for a comprehensive and integrative understanding of the overall knowledge development in the different related fields (immunology, genetics, bioinformatics, science, technology, engineering and mathematics, etc.,). In light of this, the main goal of this topical collection is to bridge the gap between all these fields. Each article in this compilation is a stand‐alone text based on an integrative review, which highlights advances in molecular diagnosis and epidemiology of human pathogens. It also helps researchers and practitioners to access and scrutinize the fast expanding knowledge. This research topic in medical microbiology provides an updated core of basic knowledge critical to clinical practice in medicine. It is designed for physicians, researchers, educators, residents, Lab technicians, undergraduate and graduate students. Each article deals with an important human pathogen. It uses a multidisciplinary approach integrating new advances, especially in genetics, bioinformatics, science, technology, engineering and mathematics. It provides an update of the current state-of-the-art scientific knowledge applied to molecular diagnosis and epidemiology of human pathogens. Rapid detection, accurate identification and typing of causative pathogens are necessary to optimize treatment, to trace pathogens and to study microbial population dynamics.
Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing.
This book examines a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.
James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...
Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).
