Continuous Culture and Extracellular Recombinant Protein Expression in Escherichia Coli
Continuous Culture and Extracellular Recombinant Protein Expression in Escherichia Coli

Author: Ram Shankar Velur Selvamani

Publisher:

Published: 2015

Total Pages: 0

ISBN-13: 9783832539511

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The expression of recombinant proteins from Escherichia coli along with the possibility of their subsequent secretion into the extracellular space is a highly desirable feature due to reasons like easier downstream purification and protection of the product from degradation by the host. This feature has been studied and characterized previously in this group the expression of Bacteriocin Release Protein (BRP) controlled by a growth-phase regulated promoter (P raisebox{-0.3ex{ scriptsize fic) that aids in the release of recombinant proteins from the periplasmic space through the outer membrane. It was of major interest to know whether BRP-mediated secretion of recombinant proteins could be adopted in a stable chemostat and if so, what kind of interplay would be seen between activity of growth-phase regulated promoters and the productivity of a chemostat process. To make this continuous process even more sustainable, alternative plasmid selection mechanisms were tested with the aim of avoiding antibiotic-based plasmid selection. Through the use of auxotrophy complementation, it was possible to maintain a previously unstable plasmid, for extended periods of continuous cultivation without any antibiotic-based selection pressure and show extracellular expression of a recombinant enzyme, thus providing an interesting new model.

Systems Biology and Biotechnology of Escherichia coli
Systems Biology and Biotechnology of Escherichia coli

Author: Sang Yup Lee

Publisher: Springer Science & Business Media

Published: 2009-03-20

Total Pages: 471

ISBN-13: 1402093942

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Systems biology is changing the way biological systems are studied by allowing us to examine the cell and organism as a whole. Systems biotechnology allows optimal design and development of upstream to downstream bioprocesses by taking a systems-approach. E. coli has been a model organism for almost all biological and biotechnological studies. This book brings together for the first time the state-of-the-art reviews by the world-leading experts on systems biology and biotechnological applications of E. coli. The topics covered include genomics and functional genomics, resources for systems biology, network analysis, genome-scale metabolic reconstruction, modelling and simulation, dynamic modelling and simulation, systems-level analysis of evolution, plasmids and expression systems, protein synthesis, production and export, engineering the central metabolism, synthetic biology, and systems metabolic engineering of E. coli. This book provides readers with guidance on how a complex biological system can be studied using E. coli as a model organism. It also presents how to perform synthetic biology and systems metabolic engineering studies on E. coli with successful examples, the approaches of which can be extended to other organisms. This book will be a complete resource for anyone interested in systems biology and biotechnology.

Physiological Stress Responses in Bioprocesses
Physiological Stress Responses in Bioprocesses

Author: Sven-Olof Enfors

Publisher: Springer Science & Business Media

Published: 2004-05-06

Total Pages: 264

ISBN-13: 9783540203117

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This review series covers trends in modern biotechnology. All aspects of this interdisciplinary technology, where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science, are treated. Electronic version available at http://link.springer.de/series/abe/

Productive Biofilms
Productive Biofilms

Author: Kai Muffler

Publisher: Springer

Published: 2014-10-15

Total Pages: 264

ISBN-13: 3319096958

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This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

New Developments and New Applications in Animal Cell Technology
New Developments and New Applications in Animal Cell Technology

Author: Otto-Wilhelm Merten

Publisher: Springer Science & Business Media

Published: 2007-05-08

Total Pages: 708

ISBN-13: 0306468603

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Animal cell technology is becoming an increasingly important part of biotechnology and many products are now used in human health care and for veterinary applications. However, there are many times more products actually in the developmental pipelines of the biotechnology industry, including various phases of clinical trials. The Proceedings of the 15th Meeting of the European Society for Animal Cell Technology (Tours, France, September 1997) presents the actual current state as well as New Developments and Applications in Animal Cell Technology for the benefit of society. These Proceedings represent both the current state and applications of animal cell technology and the way the technology is expanding into new areas to give a unique insight into new products and applications for human and animal health care.

Oxidative Folding of Proteins
Oxidative Folding of Proteins

Author: Matthias J Feige

Publisher: Royal Society of Chemistry

Published: 2018-07-30

Total Pages: 450

ISBN-13: 1782629904

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The formation of disulphide bonds is probably the most influential modification of proteins. These bonds are unique among post-translational modifications of proteins as they can covalently link cysteine residues far apart in the primary sequence of a protein. This has the potential to convey stability to otherwise marginally stable structures of proteins. However, the reactivity of cysteines comes at a price: the potential to form incorrect disulphide bonds, interfere with folding, or even cause aggregation. An elaborate set of cellular machinery exists to catalyze and guide this process: facilitating bond formation, inhibiting unwanted pairings and scrutinizing the outcomes. Only in recent years has it become clear how intimately connected this cellular machinery is with protein folding helpers, organellar redox balance and cellular homeostasis as a whole. This book comprehensively covers the basic principles of disulphide bond formation in proteins and describes the enzymes involved in the correct oxidative folding of cysteine-containing proteins. The biotechnological and pharmaceutical relevance of proteins, their variants and synthetic replicates is continuously increasing. Consequently this book is an invaluable resource for protein chemists involved in realted research and production.

Industrial Biotechnology
Industrial Biotechnology

Author: Christoph Wittmann

Publisher: John Wiley & Sons

Published: 2017-04-10

Total Pages: 789

ISBN-13: 352734179X

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The latest volume in the Advanced Biotechnology series provides an overview of the main production hosts and platform organisms used today as well as promising future cell factories in a two volume book. Alongside describing tools for genetic and metabolic engineering for strain improvement, the authors also impart topical information on computational tools, safety aspects and industrial-scale production. Following an introduction to general concepts, historical developments and future technologies, the text goes on to cover multi-purpose bacterial cell factories, including those organisms that exploit anaerobic biosynthetic power. Further chapters deal with microbes used for the production of high-value natural compounds and those obtained from alternative raw material sources, concluding with eukaryotic workhorses.

Insoluble Proteins
Insoluble Proteins

Author: Elena García-Fruitós

Publisher: Humana Press

Published: 2014-12-02

Total Pages: 0

ISBN-13: 9781493922048

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With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.

Microbial Enzymes in Bioconversions of Biomass
Microbial Enzymes in Bioconversions of Biomass

Author: Vijai Kumar Gupta

Publisher: Springer

Published: 2016-10-25

Total Pages: 345

ISBN-13: 3319436791

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This volume summarizes our current knowledge on different biomass-converting enzymes and their potential use in converting biomass into simple sugar to generate bioenergy and other value added co-/by products. It consists of 13 chapters and is divided into three parts: cellulases; hemicellulases; and lignocellulose oxidoreductases. Written by international experts, the contributions offer clear and concise descriptions of both standard and new technologies. It is an invaluable reference resource for undergraduates, post-graduates, researchers and practitioners in the field of microbial enzymes for biofuel and biorefinery applications.