Circulating Molecular Biomarkers: Next-Generation Tools for Monitoring Minimal Residual Disease in Cancer Patients
Circulating Molecular Biomarkers: Next-Generation Tools for Monitoring Minimal Residual Disease in Cancer Patients

Author: Zohreh Amoozgar

Publisher: Frontiers Media SA

Published: 2023-08-16

Total Pages: 137

ISBN-13: 283253192X

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Traditional cancer diagnosis relies on tissue biopsy, blood testing as well as medical imaging. By the detection moment, in most cases the tumor size may reach several millimeters and the risk of distant metastasis is unavoidable. Even, following drug treatment, there is no accurate and timely way to follow up the success of treatment. New reliable tumor-specific biomarkers/tools that allow non-invasive early cancer detection as well as monitoring of anti-cancer therapeutic regime is highly demanded. To tackle limitations of traditional cancer management, liquid biopsy has emerged which screen bodily fluids using ultrasensitive biosensing systems capable of simultaneous capturing and studying horizontal gene transfer (HGT) materials including circulating tumor cells (CTCs), exosomes, cell-free DNA, and apoptotic bodies. These are messengers and/or delivery systems by which tumor cells are communicating as well as transforming/sharing new phenotypes with each other resulting in drug resistance, distant metastatic outbreaks, and cancer recurrence. Acting as next-generation prognostic and therapeutic tools call for understanding the molecular mechanisms by which HGT works to tran

Minimal Residual Disease and Circulating Tumor Cells in Breast Cancer
Minimal Residual Disease and Circulating Tumor Cells in Breast Cancer

Author: Michail Ignatiadis

Publisher: Springer Science & Business Media

Published: 2012-04-23

Total Pages: 245

ISBN-13: 3642281605

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This important book provides up-to-date information on a series of topical issues relating to the approach to minimal residual disease in breast cancer patients. It first explains how the study of minimal residual disease and circulating and disseminated tumor cells (CTCs/DTCs) can assist in the understanding of breast cancer metastasis. A series of chapters then discuss the various technologies available for the detection and characterization of CTCs and DTCs, pinpointing their merits and limitations. Detailed consideration is given to the relevance of CTCs and DTCs, and their detection, to clinical research and practice. The role of other blood-based biomarkers is also addressed, and the closing chapters debate the challenges facing drug and biomarker co-development and the use of CTCs for companion diagnostic development. This book will be of interest and assistance to all who are engaged in the modern management of breast cancer.

Minimal Residual Disease Testing
Minimal Residual Disease Testing

Author: Todd E. Druley

Publisher: Springer

Published: 2018-11-15

Total Pages: 202

ISBN-13: 331994827X

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This volume provides a concise yet comprehensive overview of minimal residual disease (MRD) testing. The text reviews the history of MRD testing, MRD testing for acute lymphoblastic leukemia/lymphoma, molecular diagnostics for MRD analysis in hematopoietic malignancies, the use of "difference from normal" flow cytometry in monitoring AML response, ML-DS for measurable residual disease detection, and advancements in next generation sequencing for detecting MRD. Written by experts in the field, Minimal Residual Disease Testing: Current Innovations and Future Directions is a valuable resource for hematologists, oncologists, pathologists, and radiologists on the variety of technologies available to detect MRD and how best to integrate these platforms into clinical practice.

Clinical Molecular Diagnostics
Clinical Molecular Diagnostics

Author: Shiyang Pan

Publisher: Springer Nature

Published: 2021-07-08

Total Pages: 889

ISBN-13: 9811610371

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This book covers the discovery of molecular biomarkers, the development of laboratory testing techniques and their clinical applications, focusing on basic research to clinical practice. It introduces new and crucial knowledge and ethics of clinical molecular diagnosis. This book emphasizes the applications of clinical molecular diagnostic test on health management, especially from different diseased organs. It lets readers to understand and realize precision healthcare.

Minimal Residual Disease in Hematologic Malignancies
Minimal Residual Disease in Hematologic Malignancies

Author: Raanani P Ed

Publisher: S. Karger AG (Switzerland)

Published: 2004

Total Pages: 0

ISBN-13: 9783805577731

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Detection of minimal residual disease (MRD) is increasingly used in the management of leukemia patients. A wide variety of methods have been developed and include technologies designed to detect residual malignant cells beyond the sensitivity of conventional approaches such as morphology and banding cytogenetics in leukemia. The choice of the best method depends on the biology of the individual malignancy, i.e. on the determination of specific markers which are useful to differentiate between leukemic cells and normal hematopoiesis in leukemic patients. These markers include leukocyte differentiation antigens, fusion transcripts, transcripts overexpressed by mutated or nonmutated genes, rearranged genes, and individual markers like polymorphic repetitive DNA sequences. The major technologies for MRD detection, their advantages and disadvantages and their clinical applications are discussed in this special issue - from 'bench to bedside'. Providing a comprehensive overview on the significance of MRD in the evaluation, treatment and follow-up of hematologic malignancies, it will be of great value to hematologists, researchers interested in leukemias and lymphomas as well as laboratory technicians.

Allogeneic Stem Cell Transplantation
Allogeneic Stem Cell Transplantation

Author: Hillard M. Lazarus

Publisher: Springer Science & Business Media

Published: 2010-03-02

Total Pages: 885

ISBN-13: 1597454788

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Since the original publication of Allogeneic Stem Cell Transplantation: Clinical Research and Practice, Allogeneic hematopoietic stem cell transplantation (HSC) has undergone several fast-paced changes. In this second edition, the editors have focused on topics relevant to evolving knowledge in the field in order to better guide clinicians in decision-making and management of their patients, as well as help lead laboratory investigators in new directions emanating from clinical observations. Some of the most respected clinicians and scientists in this discipline have responded to the recent advances in the field by providing state-of-the-art discussions addressing these topics in the second edition. The text covers the scope of human genomic variation, the methods of HLA typing and interpretation of high-resolution HLA results. Comprehensive and up-to-date, Allogeneic Stem Cell Transplantation: Clinical Research and Practice, Second Edition offers concise advice on today's best clinical practice and will be of significant benefit to all clinicians and researchers in allogeneic HSC transplantation.

Childhood Acute Lymphoblastic Leukemia
Childhood Acute Lymphoblastic Leukemia

Author: Ajay Vora

Publisher: Springer

Published: 2017-04-21

Total Pages: 345

ISBN-13: 3319397087

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This book provides a comprehensive and up-to-date review of all aspects of childhood Acute Lymphoblastic Leukemia, from basic biology to supportive care. It offers new insights into the genetic pre-disposition to the condition and discusses how response to early therapy and its basic biology are utilized to develop new prognostic stratification systems and target therapy. Readers will learn about current treatment and outcomes, such as immunotherapy and targeted therapy approaches. Supportive care and management of the condition in resource poor countries are also discussed in detail. This is an indispensable guide for research and laboratory scientists, pediatric hematologists as well as specialist nurses involved in the care of childhood leukemia.

Molecular Epidemiology
Molecular Epidemiology

Author: Paul A. Schulte

Publisher: Academic Press

Published: 2012-12-02

Total Pages: 609

ISBN-13: 0323138578

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This book will serve as a primer for both laboratory and field scientists who are shaping the emerging field of molecular epidemiology. Molecular epidemiology utilizes the same paradigm as traditional epidemiology but uses biological markers to identify exposure, disease or susceptibility. Schulte and Perera present the epidemiologic methods pertinent to biological markers. The book is also designed to enumerate the considerations necessary for valid field research and provide a resource on the salient and subtle features of biological indicators.

Basic Science Methods for Clinical Researchers
Basic Science Methods for Clinical Researchers

Author: Morteza Jalali

Publisher: Academic Press

Published: 2017-03-31

Total Pages: 383

ISBN-13: 012803078X

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Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

Epigenetic Biomarkers and Diagnostics
Epigenetic Biomarkers and Diagnostics

Author: Jose Luis Garcia-Gimenez

Publisher: Academic Press

Published: 2015-12-07

Total Pages: 698

ISBN-13: 0128019212

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Epigenetic Biomarkers and Diagnostics comprises 31 chapters contributed by leading active researchers in basic and clinical epigenetics. The book begins with the basis of epigenetic mechanisms and descriptions of epigenetic biomarkers that can be used in clinical diagnostics and prognostics. It goes on to discuss classical methods and next generation sequencing-based technologies to discover and analyze epigenetic biomarkers. The book concludes with an account of DNA methylation, post-translational modifications and noncoding RNAs as the most promising biomarkers for cancer (i.e. breast, lung, colon, etc.), metabolic disorders (i.e. diabetes and obesity), autoimmune diseases, infertility, allergy, infectious diseases, and neurological disorders. The book describes the challenging aspects of research in epigenetics, and current findings regarding new epigenetic elements and modifiers, providing guidance for researchers interested in the most advanced technologies and tested biomarkers to be used in the clinical diagnosis or prognosis of disease. - Focuses on recent progress in several areas of epigenetics, general concepts regarding epigenetics, and the future prospects of this discipline in clinical diagnostics and prognostics - Describes the importance of the quality of samples and clinical associated data, and also the ethical issues for epigenetic diagnostics - Discusses the advances in epigenomics technologies, including next-generation sequencing based tools and applications - Expounds on the utility of epigenetic biomarkers for diagnosis and prognosis of several diseases, highlighting the study of these biomarkers in cancer, cardiovascular and metabolic diseases, infertility, and infectious diseases - Includes a special section that discusses the relevance of biobanks in the maintenance of high quality biosamples and clinical-associated data, and the relevance of the ethical aspects in epigenetic studies