Cell Labelling for Hepatocyte Transplantation
Cell Labelling for Hepatocyte Transplantation

Author: Juliana Puppi

Publisher:

Published: 2011

Total Pages: 416

ISBN-13:

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Hepatocyte transplantation is being evaluated as an alternative to orthotopic liver transplantation in the management of liver-based metabolic disorders and acute liver failure. The use of magnetic resonance imaging (MRI) as a non-invasive technique to monitor donor cell engraftment following transplantation was investigated. -- Studies were performed to optimise labelling conditions for human hepatocytes with clinically available superparamagnetic iron oxide nanoparticles (SPIOs). Labelling with 50 g Fe/ml of SPIOs in culture had minimal effect on cell metabolic function and allowed label incorporation at sufficient levels for in vitro detection on a 7-T MRI system. Intrasplenic transplantation of human hepatocytes labelled with SPIOs was performed in non-obese diabetic/severe combined immunodeficient (NOD-SCID) mice. Two hours after hepatocyte transplantation, SPIO-labelled hepatocytes could be imaged in the liver. -- Incorporation of the contrast agent in hepatocytes was significantly improved using the transfection agents poly-L-lysine and protamine sulphate (PS). A rat model of acute liver failure induced by D(+)-galactosamine was used to study the engraftment potential of the labelled cells in vivo. At day 7 there was a decrease in the MRI signal intensity in the liver of rats transplanted with SPIO-PS complex-labelled cells. However, histology demonstrated that the iron in the liver was in Kupffer and endothelial cells in periportal areas rather than hepatocytes, indicating clearance of labelled hepatocytes. Donor cells were detected in the spleen of animals receiving SPIO-PS labelled hepatocytes, suggesting that some of the cells were retained in the spleen in this model. -- As an alternative approach, 99mTechnetium-labelled galactosyl-serum albumin, which binds to asialoglycoprotein receptors on the surface of hepatocytes was also investigated.

Hepatocyte Transplantation
Hepatocyte Transplantation

Author: S. Gupta

Publisher: Springer Science & Business Media

Published: 2002-09-30

Total Pages: 386

ISBN-13: 9780792387763

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In recent years there has been an increasing need for transplantation, but the number of donor livers available has increased only slightly, despite intensive public relations activities. New concepts in the field of transplantation, for instance the transplantation of living donor organs or the splitting of organs, are urgently required, to safeguard the treatment of patients with severe liver disease. The development and clinical application of cell therapy for patients with liver disease could soon present a significant enhancement of the therapeutic options. The aim of such cell therapy is to repair or improve the biological function of the chronically and acutely damaged liver. Even though systematic trials are not available, individual case reports and small series already show promising clinical results. Present concepts of cell therapy for liver diseases based on the use of primary hepatocytes have recently been considerably extended through new data on the biology of stem cells. The adult haematopoetic stem cell as a pool for hepatocyte grafts - what would be the perspectives for the clinical application? This book is the proceedings of the Falk Symposium No. 126 on `Hepatocyte Transplantation' (Progress in Gastroenterology and Hepatology Part III) held in Hannover, Germany, October 2-3, 2001, and is a forum for basic research, but also for questions concerning clinical applications in the field of hepatocyte transplantation.

Hepatocyte Transplantation
Hepatocyte Transplantation

Author: Anil Dhawan

Publisher: Humana Press

Published: 2010-07-19

Total Pages: 231

ISBN-13: 9781607611721

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Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.

Ex Vivo Cell Therapy
Ex Vivo Cell Therapy

Author: Klaus Schindhelm

Publisher: Academic Press

Published: 1999

Total Pages: 392

ISBN-13:

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R.E. Nordon and K. Schindhelm, Introduction. -- L. Robb, A.G. Elefanty, and C.G. Begley, Transcriptional Control of Hematopoieses. -- R. Starr and N.A. Nicola, Cell Signaling by Hemopoietic Growth Factor Receptors. -- P.J. Simmons, D.N. Haylock, and J.-P. Lévesque, Influence of Cytokines and Adhesion Molecules on Hematopoietic Stem Cell Development. -- P.A. Rowlings, Allogeneic Hematopoietic Stem Cell Transplantation. -- U. Hahn and L.B. To, Autologous Stem Cell Transplantation. -- M.R. Vowels, Cord Blood Stem Cell Transplantation. -- S.R. Riddell, E.H. Warren, D. Lewinsohn, C. Yee, and P.D. Greenberg, Reconstitution of Immunity by Adoptive Immunotherapy with T Cells. -- L.Q. Sun, M. Miller, and G. Symonds, Exogenous Gene Transfer into Lymphoid and Hematopoietic Progenitor Cells. -- C. Dowding, T. Leemhuis, A. Jakubowski, and C. Reading, Process Development for Ex Vivo Cell Therapy. -- R.E. Nordon and K. Schindhelm, Cell Separation. -- P.W. Zandstra, C.J. Eaves, and J.M. Piret, Environ ...

Bioengineering Liver Transplantation
Bioengineering Liver Transplantation

Author: Luc J.W. Van der Laan

Publisher: MDPI

Published: 2019-11-19

Total Pages: 132

ISBN-13: 3039217445

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The aim of this Special Issue is to review, understand, and evaluate new and exciting opportunities from the field on regenerative medicine, biomaterials, and stem cell research for the bioengineering of human liver grafts that can be applied for transplantation and personalized treatment of end-stage liver disease.The development of culture conditions for long-term expansion of LGR5+ intestinal stem cells as crypt-villus structures demonstrated the feasibility of deriving complex, organ-like structures in vitro from primary adult tissues, including the liver. Moreover, human pluripotent stem cells (hPSCs) can be applied to generate functionally maturated liver and bile duct epithelial cells.In this Special Issue, we welcome reviews and original papers focussing on hepatic cell sources, including adult hepatic stem cells, organoids, fetal and induced pluripotent stem cells, and primary cells (i.e., hepatocytes, cholangiocytes, and endothelial cells) and how these cells can be applied in tissue engineering strategies to generate implantable and personalized liver grafts. Potential topics include, but are not limited to, the following: liver tissue engineering, liver regeneration, graft repair, liver stem cells and organoids, bio-scaffolds, and 3D printing.We invite you to contribute original research papers, as well as comprehensive reviews, aligned with these themes, to advance and improve the actual state-of-the-art in liver bioengineering and providing new opportunities for the imminent medical problem of organ and tissue shortage for transplantation.

Stem Cell and Liver Regeneration
Stem Cell and Liver Regeneration

Author: K. Okita

Publisher: Springer Science & Business Media

Published: 2004

Total Pages: 108

ISBN-13: 9784431401995

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Cellular therapy with liver stem cells and their progeny, including bone marrow cells, is a promising new approach that will contribute significantly to the treatment of liver diseases. The existence of the liver stem cell had long been debated, but it is now generally accepted that the liver contains cells with stem-like properties and that these cells can be activated to proliferate and differentiate into mature hepatocytes under certain conditions. There is also a substantial body of evidence to suggest that oval cells are involved in liver regeneration as they differentiate into hepatocytes and biliary cells. This book is a collection of work on stem cell and liver regeneration, initially delivered at the 14th Yamaguchi (Japan) Symposium on Liver Disease. Its nine chapters present the most recent data about basic and clinical research in hepatology in Japan and other countries, providing a valuable resource for researchers and practitioners alike.

Hepatocyte Transplantation
Hepatocyte Transplantation

Author: Anil Dhawan

Publisher: Humana Press

Published: 2009-01-28

Total Pages: 0

ISBN-13: 9781588298836

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Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.

Bioengineering Liver Transplantation
Bioengineering Liver Transplantation

Author: Luc J.W. Van der Laan

Publisher:

Published: 2019

Total Pages: 132

ISBN-13: 9783039217458

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The aim of this Special Issue is to review, understand, and evaluate new and exciting opportunities from the field on regenerative medicine, biomaterials, and stem cell research for the bioengineering of human liver grafts that can be applied for transplantation and personalized treatment of end-stage liver disease.The development of culture conditions for long-term expansion of LGR5+ intestinal stem cells as crypt-villus structures demonstrated the feasibility of deriving complex, organ-like structures in vitro from primary adult tissues, including the liver. Moreover, human pluripotent stem cells (hPSCs) can be applied to generate functionally maturated liver and bile duct epithelial cells.In this Special Issue, we welcome reviews and original papers focussing on hepatic cell sources, including adult hepatic stem cells, organoids, fetal and induced pluripotent stem cells, and primary cells (i.e., hepatocytes, cholangiocytes, and endothelial cells) and how these cells can be applied in tissue engineering strategies to generate implantable and personalized liver grafts. Potential topics include, but are not limited to, the following: liver tissue engineering, liver regeneration, graft repair, liver stem cells and organoids, bio-scaffolds, and 3D printing.We invite you to contribute original research papers, as well as comprehensive reviews, aligned with these themes, to advance and improve the actual state-of-the-art in liver bioengineering and providing new opportunities for the imminent medical problem of organ and tissue shortage for transplantation.

Split liver transplantation
Split liver transplantation

Author: X. Rogiers

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 182

ISBN-13: 3642575234

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Aimed at the trainee surgeon and experienced transplant surgeon, this compendium on split-liver grafting contains articles written by faculty members of the first International Course on Split-Liver Transplantation. It covers the main aspects of the field and is geared towards helping surgeons select the best surgical techniques as well as identifying the pitfalls. The text features detailed instructions on the various procedures as well as an overview of the area.