After a long battle, Ichigo loses his Soul Reaper powers and is now living a quiet and peaceful life. But when a mysterious man named Kugo Ginjo approaches him, Ichigo might have a chance to regain his powers. But what are Ginjo’s true motives...? -- VIZ Media
Facing a powerful opponent, the mysterious Kisuke Urahara is forced to reveal his Bankai for the first time. Meanwhile, Ichigo finally makes it to Yhwach’s throne room, but what can he do against an enemy whose power is omnipotence?! -- VIZ Media
This third edition volume expands on the previous editions with new discussion on the latest techniques and model systems used to study germline stem cells. The chapters in this book cover topics such as analysis of the C. elegans germline stem cell pool; live imaging of the Drosophila testis stem cell niche; standardization of single-cell RNA-sequencing analysis workflow to study Drosophila ovary; 3D analysis of the intact mouse ovary; and derivation and primordial germ cell induction of intermediate pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Germline Stem Cells: Methods and Protocols, Third Edition is a valuable resource for all researchers in the germ stem cell community interested in learning more about this developing field.