Colloidal Gold

Colloidal Gold

Author:

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 446

ISBN-13: 0323139019

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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. - Among the first books to cover principles and methodology of colloidal gold in microscopy - Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book - Discusses problems and limitations of techniques - Guides users to avoid problems and choose the correct procedures for specific applications - Contributors are eminent authorities in their fields - Topics covered in Volume 3 include: Production of monoclonal and polyclonal antibodies, Lowicryl, polar, and apolar resins - Preserving and localizing antigenicity; sectioning; high-resolution morphological localization - Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation - Applications to biomedical studies - Colloidal gold in conjunction with video-enhanced microscopy


NanoBiotechnology Protocols

NanoBiotechnology Protocols

Author: Sandra J Rosenthal

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 241

ISBN-13: 159259901X

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Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena.


Green Nanoparticles

Green Nanoparticles

Author: Jayanta Kumar Patra

Publisher: Springer Nature

Published: 2020-04-06

Total Pages: 395

ISBN-13: 3030392465

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Nanotechnology is the application of science to control matter at the molecular level. It has become one of the most promising applied technologies in all areas of science. Nanoparticles have multi-functional properties and have created very interesting applications in various fields such as medicine, nutrition, bioenergy, agriculture and the environment. But the biogenic syntheses of monodispersed nanoparticles with specific sizes and shapes have been a challenge in biomaterial science. Nanoparticles are of great interest due to their extremely small size and large surface-to-volume ratio, which lead to both chemical and physical differences in their properties (e.g., mechanical properties, biological and sterical properties, catalytic activity, thermal and electrical conductivity, optical absorption and melting point) compared to bulk of the same chemical composition. Recently, however, synthesizing metal nanoparticles using green technology via microorganisms, plants, viruses, and so on, has been extensively studied and has become recognized as a green and efficient way for further exploiting biological systems as convenient nanofactories. Thus the biological synthesis of nanoparticles is increasingly regarded as a rapid, ecofriendly, and easily scaled-up technology. Today researchers are developing new techniques and materials using nanotechnology that may be suitable for plants to boost their native functions. Recently, biological nanoparticles were found to be more pharmacologically active than physico-chemically synthesized nanoparticles. Various applications of biosynthesized nanoparticles have been discovered, especially in the field of biomedical research, such as applications to specific delivery of drugs, use for tumor detection, angiogenesis, genetic disease and genetic disorder diagnosis, photoimaging, and photothermal therapy. Further, iron oxide nanoparticles have been applied to cancer therapy, hyperthermia, drug delivery, tissue repair, cell labeling, targeting and immunoassays, detoxification of biological fluids, magnetic resonance imaging, and magnetically responsive drug delivery therapy. Nanoparticle synthesis for plant byproducts for biomedical applications has vast potential. This book offers researchers in plant science and biomedicine the latest research and opportunity to develop new tools for the synthesis of environmentally friendly and cost-effective nanoparticles for applications in biomedicine as well as other various fields.


Microbial Interactions at Nanobiotechnology Interfaces

Microbial Interactions at Nanobiotechnology Interfaces

Author: R. Navanietha Krishnaraj

Publisher: John Wiley & Sons

Published: 2021-11-02

Total Pages: 420

ISBN-13: 1119617197

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MICROBIAL INTERACTIONS AT NANOBIOTECHNOLOGY INTERFACES This book covers a wide range of topics including synthesis of nanomaterials with specific size, shape, and properties, structure-function relationships, tailoring the surface of nanomaterials for improving the properties, interaction of nanomaterials with proteins/microorganism/eukaryotic cells, and applications in different sectors. This book also provides a strong foundation for researchers who are interested to venture into developing functionalized nanomaterials for any biological applications in their research. Practical concepts such as modelling nanomaterials, and simulating the molecular interactions with biomolecules, transcriptomic or genomic approaches, advanced imaging techniques to investigate the functionalization of nanomaterials/interaction of nanomaterials with biomolecules and microorganisms are some of the chapters that offer significant benefits to the researchers.


Making and Using Antibodies

Making and Using Antibodies

Author: Gary C. Howard

Publisher: CRC Press

Published: 2013-07-29

Total Pages: 446

ISBN-13: 1439869103

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Antibodies protect us from a wide range of infectious diseases and cancers and have become an indispensable tool in science-both for conventional immune response research as well as other areas related to protein identification analysis. This second edition of Making and Using Antibodies: A Practical Handbook provides clear guidance on all aspects


Nanobiotechnology

Nanobiotechnology

Author: Sougata Ghosh

Publisher: Elsevier

Published: 2021-05-20

Total Pages: 362

ISBN-13: 0128231157

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Nanobiotechnology: Microbes and Plant Assisted Synthesis of Nanoparticles, Mechanisms and Applications covers in detail the green synthesis of nanostructures of tailor-made size, shape and physico-chemical and opto-electronic properties. The rationale behind the selection of bacteria, cyanobacteria, algae, fungi, virus and medicinal plants for the synthesis of biologically active exotic nanoparticles for biomedical applications is also part of this book. It also explores metal recovery, bioconversion, detoxification and removal of heavy metals using nanobiotechnology and discusses the potential of nanobiotechnology to address environmental pollution and toxicity. The book further covers the economic and commercial aspects of such green nanobiotechnology initiatives, its current status in intellectual property rights like patents filed so far globally, technology transfers, and market potential. This information enables one to decipher the scope of biogenic nanoparticles and its prospects. - Provides an overview on the general and applied aspects on nanotechnology - Gives the scope of exploring bacteria, fungi, algae, virus and medicinal plants for the synthesis of exotic nanoparticles - Furnishes a comprehensive report on the underlying molecular mechanisms behind the biosynthesis of nanoparticles - Outlines sustainable alternative strategies of bioremediation of heavy metals, metal recovery, detoxification and bioconversion using nanobiotechnology - Explores the promises of patenting, technology transfer and commercialization potential of biogenic nanoparticles


Synthesis of Bionanomaterials for Biomedical Applications

Synthesis of Bionanomaterials for Biomedical Applications

Author: Munir Ozturk

Publisher: Elsevier

Published: 2023-01-11

Total Pages: 580

ISBN-13: 0323998437

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Synthesis of Bionanomaterials for Biomedical Applications summarizes a range of procedures, including green synthesis of metal nanoparticles, metal oxide nanoparticles, and other types of nanoparticles while also exploring the appropriate use of these nanoparticles in various therapeutic applications such as anticancer, antibacterial, antifungal, drug delivery, and more. The book provides important information for materials scientists and pharmaceutical scientists on the synthesis of various nanoparticles using a variety of eco-friendly bionanomaterials. As concern has arisen regarding the environmental impact caused by some of nanomaterials, as well as their possible toxicity to cells, this book presents information on a new generation of eco-friendly materials. In addition, the green synthesis of nanoparticles shows how environmentally-friendly nanoparticles can be synthesized from different biological sources, such as microbes, fungi, algae and plants. - Provides information on the synthesis and application of eco-friendly bionanomaterials - Offers coverage of nanomaterials generated through green synthesis - Assesses the challenges of manufacturing eco-friendly nanomaterials on an industrial scale


Biological Low-Voltage Scanning Electron Microscopy

Biological Low-Voltage Scanning Electron Microscopy

Author: James Pawley

Publisher: Springer Science & Business Media

Published: 2007-12-03

Total Pages: 323

ISBN-13: 0387729720

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Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.