Comprehensive, Up-to-Date Coverage of Spectroscopy Theory and its Applications to Biological SystemsAlthough a multitude of books have been published about spectroscopy, most of them only occasionally refer to biological systems and the specific problems of biomolecular EPR (bioEPR). Biomolecular EPR Spectroscopy provides a practical introduction t
EPR Spectroscopy in Catalysis, by Sabine Van Doorslaer und Damien M. Murphy Radicals in Flavoproteins, by Erik Schleicher und Stefan Weber EPR Spectroscopy in Polymer Science, by Dariush Hinderberger EPR in Protein Science, by Intrinsically Disordered Proteins, by Malte Drescher Site-Directed Spin Labeling of Membrane Proteins, by Enrica Bordignon Structure and Dynamics of Nucleic Acids, by Ivan Krstić, Burkhard Endeward, Dominik Margraf, Andriy Marko und Thomas F Prisner New Directions in Electron Paramagnetic Resonance Spectroscopy on Molecular Nanomagnets, by J. van Slageren
The technique of nuclear magnetic resonance (NMR) spectroscopy is an important tool in biochemistry and biophysics for the understanding of the structure and ultimately, the function of biomolecules. This textbook explains the salient features of biological NMR spectroscopy to undergraduates and postgraduates taking courses in NMR, biological NMR, physical biochemistry, and biophysics. Unlike other books in the general field of NMR (except the advanced treatises), the approach here is tointroduce and make use of quantum mechanical product operators as well as the classical vector method of explaining the bewildering array of pulse sequences available today. The book covers two- dimensional, three- dimensional, and four- dimensional NMR and their application to protein and DNA structure determination. A unique feature is the coverage of the biological aspects of solid- state NMR spectroscopy. The author provides many selected examples from the research literature, illustratingthe applications of NMR spectroscopy to biological proteins.
Spin-label electron paramagnetic resonance (EPR) spectroscopy is a versatile molecular probe method that finds wide application in molecular biophysics and structural biology. This book provides the first comprehensive summary of basic principles, spectroscopic properties, and use for studying biological membranes, protein folding, supramolecular structure, lipid-protein interactions, and dynamics. The contents begin with discussion of fundamental theory and practice, including static spectral parameters and conventional continuous-wave (CW) spectroscopy. The development then progresses, via nonlinear CW-EPR for slower motions, to the more demanding time-resolved pulse EPR, and includes an in-depth treatment of spin relaxation and spectral line shapes. Once the spectroscopic fundamentals are established, the final chapters acquire a more applied character. Extensive appendices at the end of the book provide detailed summaries of key concepts in magnetic resonance and chemical physics for the student reader and experienced practitioner alike. Key Features: Indispensable reference source for the understanding and interpretation of spin-label spectroscopic data in its different aspects. Tables of fundamental spectral parameters are included throughout. Forms the basis for an EPR graduate course, extending up to a thorough coverage of advanced topics in Specialist Appendices. Includes all necessary theoretical background. The primary audience is research workers in the fields of molecular biophysics, structural biology, biophysical chemistry, physical biochemistry and molecular biomedicine. Also, physical chemists, polymer physicists, and liquid-crystal researchers will benefit from this book, although illustrative examples used are often taken from the biomolecular field. Readers will be postgraduate researchers and above, but include those from other disciplines who seek to understand the primary spin-label EPR literature.
Electron paramagnetic resonance (EPR) applications remain highly significant in modern analytical science and this volume compiles critical coverage of developments in the recent literature. The topics covered in this volume describe contrasting types of EPR application, including rapid scan EPR, using the EPR toolkit to investigate the structural dynamics of membrane proteins and pulse dipolar EPR spectroscopy for investigating biomolecular binding events. An additional chapter reviewing the PARACAT collaboration from the EU has also been included. Providing a snapshot of the area by a handpicked group of researchers at the cutting-edge of the field, this book is a useful addition to any library supporting this research.
Spin Resonance Spectroscopy: Principles and Applications presents the principles, recent advancements and applications of nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) in a single multi-disciplinary reference. Spin resonance spectroscopic techniques through NMR and EPR are widely used by chemists, physicists, biologists and medicinal chemists. This book addresses the need for new spin resonance spectroscopy content while also presenting the principles, recent advancements and applications of NMR and EPR simultaneously. Ideal for researchers and students alike, the book provides a single source of NMR and EPR applications using a dynamic, holistic and multi-disciplinary approach. - Presents a highly interdisciplinary approach by including NMR and EPR applications in chemistry, physics, biology and biotechnology - Addresses both NMR and EPR, making its concepts and applications implementable in multiple resonance environments and core scientific disciplines - Features a broad range of methods, examples and illustrations for both NMR and EPR to aid in retention and underscore key concepts
Ribozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.
Biomolecular Structure and Function covers the proceedings of the 1977 ?Cellular Function and Molecular Structure: Biophysical Approaches to Biological Problems? symposium. It summarizes the application of several biophysical techniques to molecular research in biology. This book starts by describing the use of deuterium-labeled lipids, as monitors of the degree of organization of membrane lipids. It also describes the use of carbon-13-labeled lipids, as indicators of molecular mobility. It explains the lipid-protein interactions involving two integral membrane proteins, mitochondrial cytochrome oxidase and calcium-dependent ATPase of muscle sarcoplasmic reticulum. The book goes on to present NMR studies on the organization and conformation of phospholipids, chloroplast membranes, and erythrocyte membranes. It also presents the ESR study of spectrin-phospholipid associations. It discusses the use of fluorescence probes, electrokinetics, neutron diffraction and ion theory studies of phospholipid-protein association, hormone disease, and senescence effects on prokaryotic and eukaryotic cells. Moreover, this book presents the experiments and phosphorus-31 NMR methodology to simultaneously monitor the intracellular pH and phosphate metabolism in a beating heart, functioning kidney, or an intact living microorganism. This book then describes physical probing of intracellular fluidity and structural changes attending tissue or cell cycles. It also relates relatively narrow lines in the hydrogen-1 NMR spectrum of the extremely viscous complex of the muscle protein troponin and highly polymerized tropomyosin. Structure-function studies of fibrous proteins, such as collagen, actin, and myosin, and active site analysis of enzymes are also presented. Finally, a wide variety of methodologies and technologies is exemplified. This includes proton, carbon, fluorine, phosphorus, and lithium NMR spectroscopy; spin labeling and EPR spectroscopy; chemical studies; light scattering and fluorescence; and electron microscopy.
This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.
Stable radicals - molecules with odd electrons which are sufficiently long lived to be studied or isolated using conventional techniques - have enjoyed a long history and are of current interest for a broad array of fundamental and applied reasons, for example to study and drive novel chemical reactions, in the development of rechargeable batteries or the study of free radical reactions in the body. In Stable Radicals: Fundamentals and Applied Aspects of Odd-Electron Compounds a team of international experts provide a broad-based overview of stable radicals, from the fundamental aspects of specific classes of stable neutral radicals to their wide range of applications including synthesis, materials science and chemical biology. Topics covered include: triphenylmethyl and related radicals polychlorinated triphenylmethyl radicals: towards multifunctional molecular materials phenalenyls, cyclopentadienyls, and other carbon-centered radicals the nitrogen oxides: persistent radicals and van der Waals complex dimers nitroxide radicals: properties, synthesis and applications the only stable organic sigma radicals: di-tert-alkyliminoxyls. delocalized radicals containing the hydrazyl [R2N-NR] unit metal-coordinated phenoxyl radicals stable radicals containing the thiazyl unit: synthesis, chemical, and materials properties stable radicals of the heavy p-block elements application of stable radicals as mediators in living-radical polymerization nitroxide-catalyzed alcohol oxidations in organic synthesis metal-nitroxide complexes: synthesis and magneto-structural correlations rechargeable batteries using robust but redox-active organic radicals spin labeling: a modern perspective functional in vivo EPR spectroscopy and imaging using nitroxides and trityl radicals biologically relevant chemistry of nitroxides Stable Free Radicals: Fundamentals and Applied Aspects of Odd-Electron Compounds is an essential guide to this fascinating area of chemistry for researchers and students working in organic and physical chemistry and materials science.
