The Fusarium Laboratory Manual

The Fusarium Laboratory Manual

Author: John F. Leslie

Publisher: John Wiley & Sons

Published: 2008-02-28

Total Pages: 402

ISBN-13: 0470276460

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For the first time in over 20 years, a comprehensive collection of photographs and descriptions of species in the fungal genus Fusarium is available. This laboratory manual provides an overview of the biology of Fusarium and the techniques involved in the isolation, identification and characterization of individual species and the populations in which they occur. It is the first time that genetic, morphological and molecular approaches have been incorporated into a volume devoted to Fusarium identification. The authors include descriptions of species, both new and old, and provide protocols for genetic, morphological and molecular identification techniques. The Fusarium Laboratory Manual also includes some of the evolutionary biology and population genetics thinking that has begun to inform the understanding of agriculturally important fungal pathogens. In addition to practical “how-to” protocols it also provides guidance in formulating questions and obtaining answers about this very important group of fungi. The need for as many different techniques as possible to be used in the identification and characterization process has never been greater. These approaches have applications to fungi other than those in the genus Fusarium. This volume presents an introduction to the genus Fusarium, the toxins these fungi produce and the diseases they can cause. "The Fusarium Laboratory Manual is a milestone in the study of the genus Fusarium and will help bridge the gap between morphological and phylogenetic taxonomy. It will be used by everybody dealing with Fusarium in the Third Millenium." --W.F.O. Marasas, Medical Research Council, South Africa


Molecular Biology Techniques

Molecular Biology Techniques

Author: Heather B. Miller

Publisher: Academic Press

Published: 2011-10-18

Total Pages: 227

ISBN-13: 0123855454

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions


Investigating Biology Laboratory Manual

Investigating Biology Laboratory Manual

Author: Judith Giles Morgan

Publisher: Benjamin-Cummings Publishing Company

Published: 2010

Total Pages: 0

ISBN-13: 9780321668219

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With its distinctive investigative approach to learning, this best-selling laboratory manual encourages you to participate in the process of science and develop creative and critical reasoning skills. You are invited to pose hypotheses, make predictions, conduct open-ended experiments, collect data, and apply the results to new problems. The Seventh Edition emphasizes connections to recurring themes in biology, including structure and function, unity and diversity, and the overarching theme of evolution. Select tables from the lab manual are provided in Excel® format in MasteringBiology® at www.masteringbiology.com, allowing you to record data directly on their computer, process data using statistical tests, create graphs, and be prepared to communicate your results in class discussions or reports.


Animal Contests

Animal Contests

Author: Ian C. W. Hardy

Publisher: Cambridge University Press

Published: 2013-05-30

Total Pages: 385

ISBN-13: 1107244390

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Contests are an important aspect of the lives of diverse animals, from sea anemones competing for space on a rocky shore to fallow deer stags contending for access to females. Why do animals fight? What determines when fights stop and which contestant wins? Addressing fundamental questions on contest behaviour, this volume presents theoretical and empirical perspectives across a range of species. The historical development of contest research, the evolutionary theory of both dyadic and multiparty contests, and approaches to experimental design and data analysis are discussed in the first chapters. This is followed by reviews of research in key animal taxa, from the use of aerial displays and assessment rules in butterflies and the developmental biology of weapons in beetles, through to interstate warfare in humans. The final chapter considers future directions and applications of contest research, making this a comprehensive resource for both graduate students and researchers in the field.


Plant Molecular Biology — A Laboratory Manual

Plant Molecular Biology — A Laboratory Manual

Author: Melody S. Clark

Publisher: Springer Science & Business Media

Published: 2013-11-27

Total Pages: 551

ISBN-13: 3642878733

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Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.


Techniques in Organic Chemistry

Techniques in Organic Chemistry

Author: Jerry R. Mohrig

Publisher: Macmillan

Published: 2010-01-06

Total Pages: 487

ISBN-13: 1429219564

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"Compatible with standard taper miniscale, 14/10 standard taper microscale, Williamson microscale. Supports guided inquiry"--Cover.


Forensic DNA Biology

Forensic DNA Biology

Author: Kelly M. Elkins

Publisher: Academic Press

Published: 2012-09-11

Total Pages: 226

ISBN-13: 0123945852

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A collection of forensic DNA typing laboratory experiments designed for academic and training courses at the collegiate level.