Arabidopsis Protocols
Arabidopsis Protocols

Author: Jose J. Sanchez-Serrano

Publisher: Methods in Molecular Biology

Published: 2013-09-21

Total Pages: 754

ISBN-13:

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Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome. These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future. In addition, considering the recent unparalleled progress of the “omics” tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Arabidopsis thaliana is acknowledged as the most important plant model system by the scientific community and Arabidopsis research has fundamentally influenced our understanding of the basic biology and ecology of plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Arabidopsis Protocols, Third Edition seeks to serve both experienced researchers and beginners with its detailed methodologies on this burgeoning scientific field.

Arabidopsis
Arabidopsis

Author: John Bowman

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 431

ISBN-13: 1461225981

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The recent application of molecular genetics to problems of developmental biology has provided us with greater insight into the molecular mechanisms by which cells determine their developmental fate. This is particularly evident in the recent progress in understanding of developmental processes in model animal systems such as Drosophila melanogaster and Caenorhabditis elegans. De spite the use of plants in some of the earliest genetics experiments, the elucida tion of the molecular bases of plant development has lagged behind that of animal development. However, the emergence of model systems such as Arabi dopsis thaliana, amenable to developmental genetics, has led to the beginning of the unraveling of the mysteries behind plant morphogenesis. This atlas of the morphology and development of the weed Arabidopsis is in tended to be a reference book, both for scientists already familiar with plant anatomy and for those utilizing Arabidopsis who have come from other fields. The primary concentration is on descriptions rather than interpretations, as interpretations evolve and change relatively rapidly, whereas the evolution of plant form takes place on a much longer time scale. Molecular genetics and the use of mutants to probe wild-type gene function rely on the wild-type being well characterized. With this in mind, an attempt was made to present detailed descriptions of wild-type structure and development, to provide a foundation for comparison with the selected mutants in the atlas. More importantly, it is hoped that the atlas will serve as a valuable resource in the characterization of new mutants.

Biochemistry and Molecular Biology of Plants
Biochemistry and Molecular Biology of Plants

Author: Bob B. Buchanan

Publisher: John Wiley & Sons

Published: 2015-08-31

Total Pages: 1280

ISBN-13: 0470714220

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With over 1000 original drawings and 500 photographs, this work offers complete coverage of cell biology, plant physiology and molecular biology.

Arabidopsis
Arabidopsis

Author: Zoe Wilson

Publisher: OUP Oxford

Published: 2000-01-06

Total Pages: 298

ISBN-13: 0191565547

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Arabidopsis has long been acknowledged as the 'Botanical Drosophila' with its small genome, low levels of repetitive DNA, small size and fast generation time it is an ideal molecular genetic tool for the analysis of development in higher plants. Arabidopsis: A Practical Approach provides an introduction to most of the key techniques required for the use of Arabidopsis as an experimental system. It gives a basic introduction to the optimal growth conditions and genetic resources available for Arabidopsis, how this material should be handled, maintained and used. Individual chapters describe strategies for the identification, mapping (using multi-marker lines and recombinant inbreds), and characterisation of different mutants by microscopy, molecular cytogenetics and gene expression analysis. Different cloning strategies, using transposons, T-DNA and map position are described in detail. Sequencing of the Arabidopsis genome will be completed in 2000 and bioinformatics are of key importance; the tools that are available and where they can be found on the Web are presented.

Ecological Genomics
Ecological Genomics

Author: Christian R. Landry

Publisher: Springer Science & Business Media

Published: 2013-11-25

Total Pages: 358

ISBN-13: 9400773471

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Researchers in the field of ecological genomics aim to determine how a genome or a population of genomes interacts with its environment across ecological and evolutionary timescales. Ecological genomics is trans-disciplinary by nature. Ecologists have turned to genomics to be able to elucidate the mechanistic bases of the biodiversity their research tries to understand. Genomicists have turned to ecology in order to better explain the functional cellular and molecular variation they observed in their model organisms. We provide an advanced-level book that covers this recent research and proposes future development for this field. A synthesis of the field of ecological genomics emerges from this volume. Ecological Genomics covers a wide array of organisms (microbes, plants and animals) in order to be able to identify central concepts that motivate and derive from recent investigations in different branches of the tree of life. Ecological Genomics covers 3 fields of research that have most benefited from the recent technological and conceptual developments in the field of ecological genomics: the study of life-history evolution and its impact of genome architectures; the study of the genomic bases of phenotypic plasticity and the study of the genomic bases of adaptation and speciation.

Methods In Arabidopsis Research
Methods In Arabidopsis Research

Author: Nam-hai Chua

Publisher: World Scientific Publishing Company

Published: 1992-06-12

Total Pages: 496

ISBN-13: 9813103426

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One of the ways to make consistent progress in a particular field of biology consists in choosing a good model system on which to focus the experimental efforts of the scientific community. It has taken a long time for scientists interested in various aspects of the life of plants to reach some sort of consensus. With the advent and impact of molecular biology, the small weed Arabidopsis is now the object of rapidly growing scientific attention. Since it is reasonable to assume that the general molecular mechanisms that are responsible for the physiological, cellular and biochemical properties of plants will be essentially conserved in all plants, it follows that these mechanisms should also operate in Arabidopsis and hence that its genome should contain most of the genes that we need to know about if we want to understand the genetic determination of the life processes in plants.Arabidopsis has a small genome and well documented genetic studies are available. It is easy to grow in large numbers and mutants defining important genetically controlled mechanisms are either available, or can readily be obtained. Various methods to introduce and express isolated homologous or heterologous genes are available. It is therefore realistic and desirable to aim at exploring the genome of this plant in very great detail. As will be illustrated in this book all the elements for such a grand strategy are in place.More and more scientists are therefore willing to accept the obvious and very real practical disadvantages resulting from its small size when experiments call for the isolation of proteins, membranes, subcellular fractions etc, in order to benefit from its extraordinary experimental advantages as a model system in molecular genetics. One can safely predict that in the next decade studies with Arabidopsis will provide major breakthroughs in our understanding of most aspects of plant physiology and developmental biology. The importance of this knowledge for plant breeding and therefore for a sustainable highly productive agriculture cannot be overestimated. We therefore expect that this book will provide valuable guidelines to all those who are planning experiments aimed at understanding various aspects of plant growth, productivity and interactions with the environment. The book offers a wealth of methodical and theoretical information as well as valuable references. It should be of use to students, teachers, as well as advanced researchers and those breeders who want to use molecular techniques in breeding.

Abscisic Acid in Plants
Abscisic Acid in Plants

Author:

Publisher: Academic Press

Published: 2019-11-21

Total Pages: 386

ISBN-13: 0081026218

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Abscisic Acid in Plants, Volume 92, the latest release in the Advances in Botanical Research series, is a compilation of the current state-of-the-art on the topic. Chapters in this new release comprehensively describe latest knowledge on how ABA functions as a plant hormone. They cover topics related to molecular mechanisms as well as the biochemical and chemical aspects of ABA action: hormone biosynthesis, catabolism, transport, perception, signaling in plants, seeds and in response to biotic and abiotic stresses, hormone evolution and chemical biology, and much more. - Presents the latest release in the Advances in Botanical Research series - Provides an Ideal resource for post-graduates and researchers in the plant sciences, including plant physiology, plant genetics, plant biochemistry, plant pathology, and plant evolution - Contains contributions from internationally recognized authorities in their respective fields

Arabidopsis Thaliana
Arabidopsis Thaliana

Author: Adriano Sofo

Publisher: Nova Science Publishers

Published: 2015-05-07

Total Pages: 182

ISBN-13: 9781634821964

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Plants are sessile organisms and their only alternative to a rapidly changing environment is a fast adaptation to abiotic and biotic stresses. Among the several known species of flowering plants, Arabidopsis thaliana is the only plant that has been most thoroughly studied. This angiosperm with dicotyledonous seeds belonging to the family Brassicaceae was known to botanists for at least four centuries and has been used since then for experimental studies for about half a century, until it was Fried rich Laibach who had outlined the advantages of using it in genetic experiments and had also suggested that it could be used as a plant model system in 1943. Its unique features favors genetic experiments, which include its small size, a rapid generation time, the ability to grow well under controlled conditions, high fecundity if up to 10,000 seeds per plant. Like the peas that Mendel studied, it reproduces mainly by self-fertilization. Arabidopsis is considered a model plant for many studies as its genomic sequence was completely identified and its mechanisms in genomic, transcriptomic and proteomic regulation are often similar to other plant species. The aim of this book is to give an up-to-date overview on the recent breakthroughs in the area of responses and adaptations of Arabidopsis, particularly those regarding its cultivation, life cycle and functional genomics. The chapters are focused on the most exciting and innovative researches on this species, involving authors with strong research experience. The present volume would definitely be an ideal source of scientific information to the advanced students, junior researchers, faculty and scientists involved in the ecology, agriculture, environmental microbiology, genetics, molecular biology, biochemistry, biotechnology and other areas involving Arabidopsis studies and plant sciences in general.

The Plant Cell Cycle
The Plant Cell Cycle

Author: Dirk Inzé

Publisher: Springer Science & Business Media

Published: 2011-06-27

Total Pages: 240

ISBN-13: 9401009368

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In recent years, the study of the plant cell cycle has become of major interest, not only to scientists working on cell division sensu strictu , but also to scientists dealing with plant hormones, development and environmental effects on growth. The book The Plant Cell Cycle is a very timely contribution to this exploding field. Outstanding contributors reviewed, not only knowledge on the most important classes of cell cycle regulators, but also summarized the various processes in which cell cycle control plays a pivotal role. The central role of the cell cycle makes this book an absolute must for plant molecular biologists.

Hormone Metabolism and Signaling in Plants
Hormone Metabolism and Signaling in Plants

Author: Jiayang Li

Publisher: Academic Press

Published: 2017-04-19

Total Pages: 618

ISBN-13: 0128115637

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Plant Hormones: Biosynthesis and Mechanisms of Action is based on research funded by the Chinese government's National Natural Science Foundation of China (NSFC). This book brings a fresh understanding of hormone biology, particularly molecular mechanisms driving plant hormone actions. With growing understanding of hormone biology comes new outlooks on how mankind values and utilizes the built-in potential of plants for improvement of crops in an environmentally friendly and sustainable manner. This book is a comprehensive description of all major plant hormones: how they are synthesized and catabolized; how they are perceived by plant cells; how they trigger signal transduction; how they regulate gene expression; how they regulate plant growth, development and defense responses; and how we measure plant hormones. This is an exciting time for researchers interested in plant hormones. Plants rely on a diverse set of small molecule hormones to regulate every aspect of their biological processes including development, growth, and adaptation. Since the discovery of the first plant hormone auxin, hormones have always been the frontiers of plant biology. Although the physiological functions of most plant hormones have been studied for decades, the last 15 to 20 years have seen a dramatic progress in our understanding of the molecular mechanisms of hormone actions. The publication of the whole genome sequences of the model systems of Arabidopsis and rice, together with the advent of multidisciplinary approaches has opened the door to successful experimentation on plant hormone actions. - Offers a comprehensive description of all major plant hormones including the recently discovered strigolactones and several peptide hormones - Contains a chapter describing how plant hormones regulate stem cells - Offers a fresh understanding of hormone biology, particularly molecular mechanisms driving plant hormone actions - Discusses the built-in potential of plants for improvement of crops in an environmentally friendly and sustainable manner