This book is divided into chapters covering instrumentation, sedimentation velocity runs, density gradient runs, application examples and future developments. In particular, the detailed application chapter demonstrates the versatility and power of AUC by means of many interesting and important industrial examples. Thus the book concentrates on practical aspects rather than details of centrifugation theory.
The basis for this volume is the 11th Symposium on Analytical Ultracentrifugation held in March 25-26, 1999 at the University of Potsdam, Germany. This book presents a comprehensive collection of 33 contributions from leading scientists in this field including: Technical and methodological innovations.- Innovations in data analysis.- Hydrodynamics/Modelling.- Synthetic polymers, colloids and supramolecular systems.- Biological systems.- Interacting systems and assemblies. In contrast to the increasing significance of analytical ultracentrifugation, related modern books are very rare. Therefore, this volume will be a helpful source of information to anyone who wants to catch up with the most recent developments and results related to this important analytical method.
Analytical ultracentrifugation is one of the most powerful solution techniques for the study of macromolecular interactions, to define the number and stoichiometry of complexes formed, and to measure affinities ranging from very strong to very weak and repulsive. Building on the data analysis tools described in the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles, and the experimental and instrumental aspects in the first volume Basic Principles of Analytical Ultracentrifugation, the present volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems is devoted to the theory and practical data analysis of dynamically coupled sedimentation processes. This volume is designed to fill a gap in biophysical methodology to provide a framework that builds on the fundamentals of the highly developed traditional methods of analytical ultracentrifugation, updated with current methodology and from a viewpoint of modern applications. It will be an invaluable resource for researchers and graduate students interested in the application of analytical ultracentrifugation in the study of interacting systems, such as biological macromolecules, multi-protein complexes, polymers, or nanoparticles.
This volume includes 20 contributions of the 12th meeting on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. Various fields of ultracentrifugation are covered concerning research problems in biochemistry, biophysical chemistry and macromolecular chemistry as well as interacting systems. New investigations concerning the sedimentation theory are presented. The phase transition of gels is dealt with, as is the sedimentation-diffusion equilibrium of gels. One section contains the hydrodynamics of biopolymers.
This book introduces analytical ultracentrifugation (AUC) as a whole, covering essential theoretical and practical aspects as well as its applications in both biological and non-biological systems. Comprehensive characterizations of macromolecules in a solution are now routinely required not only for understanding the solution system but also for producing a solution with better properties. Analytical ultracentrifugation is one of most powerful and reliable techniques for studying the biophysical behavior of solutes in solution. In the last few years, there have been steady advances made in hardware, software, and applications for AUC. This book provides chapters that cover everything essential for beginners to the most advanced users and also offer updated knowledge of the field on advances in hardware, software, and applications. Recent development of hardware described in this book covers new detection systems that give added dimensions to AUC. Examples of data analysis with essential theoretical explanations for advanced and recently updated software are also introduced. Besides AUC of biological systems including membrane proteins and biopharmaceuticals, AUC applications for non-biological questions are included. AUC studies under non-ideal conditions such as highly concentrated solutions and solutions with high salt concentration are also included. The contributors to this book are leading researchers in the fields of solution biophysics and physical chemistry who extensively employ AUC analysis for their research. From this published work, one can gain new and comprehensive knowledge of recent AUC analysis.
A follow-up to the experimental and instrumental aspects described in Basic Principles of Analytical Ultracentrifugation, the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles describes the theory and practice of data analysis. Mathematical models for the sedimentation process and the evolution of detected signals are developed in a comprehensive framework, jointly with the description of current and historical strategies for how to extract from noisy experimental data the physical parameters of interest, such as size, mass, and shape, composition, and polydispersity of sedimenting particles. The methods are extensively illustrated, and supported with practical applications, as well as cross-references where to find the methods in the public domain software SEDFIT and SEDPHAT. The systems covered are discrete or polydisperse mixtures of sedimenting molecules or particles in dilute solution, such as proteins and other biomolecules and their stable complexes, man-made polymers, and nanoparticles, observed in different optical systems. A useful reference for researchers and graduate students of macromolecular disciplines, these methods form the essential foundation for the analysis of dynamic interacting systems, which are covered in the volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems. Software referenced in the book is available for download at: https://sedfitsedphat.nibib.nih.gov/default.aspx
This volume includes 19 contributions to the 13th International Symposium on Analytical Ultracentrifugation which took place at the university of Osnabrück on March 6th and 7th, 2003. The contributions from leading scientists cover a broad spectrum of topics concerning: Technical Methods, Data Analysis, Innovations; Polymers, Colloids, Supramolecular Systems; Biological and Interaction Systems; Hydrodynamics and Modelling. Due to the increasing significance of Analytical Ultracentrifugation for both scientific and technical applications, this book will be an essential source of information with respect to recent developments and results related to this important analytical method.
This is the first book of its kind to appear for nearly two decades and gives as comprehensive a coverage as is possible of the present state-of-the-art.
Analytical ultracentrifugation (AUC) can supply rich information on the mass, shape, size distribution, solvation, and composition of macromolecules and nanoscopic particles. It also provides a detailed view of their reversible single- or multi-component interactions over a wide range of affinities. Yet this powerful technique has been hard to mast
