This introduction explains what centrifugation is, what it does, how it does it, and the types of centrifuges, rotors, techniques, and density-gradient media currently available.
An important introduction to the use of the centrifuge in the biology laboratory, Biological Centrifugation is also useful for more experienced workers. The book describes the background and the principles behind centrifugation, including sedimentation theory. The book also considers the different types of centrifuge and other centrifuge hardware available, density gradient media and gradient technology. Although aimed primarily at the novice, this title also provides information to allow more experienced workers to modify and update existing techniques.
This edition provides extensive experimental details of protocols for all types of centrifugal separations from macrmolecules to whole cells. It also describes the applications of centrifuges ranging from simle bench machines to analytical centrifuges.
This book is both an important introduction to the use of the centrifuge in the biological laboratory, and a useful guide for more experienced researchers. It describes the background and principles behind centrifugation, including sedimentation theory. It also considers the different types of centrifuges and centrifuge hardware available, density gradient media and gradient technology.
Centrifugal Separations in Biotechnology, Second Edition, is the only book on the market devoted to centrifugal separation in biotechnology. Key topics covered include a full introduction to centrifugation, sedimentation and separation; detailed coverage of centrifuge types, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and pilot testing of centrifuges; selection and sizing centrifuges; scale-up of equipment, performance prediction and analysis of test results using numerical simulation. Centrifugal Separations in Biotechnology, Second Edition, provides guidance on troubleshooting and optimizing centrifuges, and then goes on to explore the commercial applications of centrifuges in biotechnology. It gives detailed process information and data to assist in the development of particular processes from existing systems. It is of value to professionals in the chemical, bioprocess, and biotech sectors, and all those concerned with bioseparation, bioprocessing, unit-operations and process engineering. Provides a comprehensive guide to centrifuges, their optimal development, and their operation in the biotechnology industry Updated throughout based on developments in industrial applications and advances in our understanding of centrifugal separations in biotechnology Discusses applications for the separation of proteins, DNA, mitochondria, ribosomes, lysosomes and other cellular elements Includes new sections on use of optimal polymer dosage in waste treatment, new centrifuge designs for applications in algae processing, biopharma, and more
An important introduction to the use of the centrifuge in the biology laboratory, Biological Centrifugation is also useful for more experienced workers. The book describes the background and the principles behind centrifugation, including sedimentation theory. The book also considers the different types of centrifuge and other centrifuge hardware available, density gradient media and gradient technology. Although aimed primarily at the novice, this title also provides information to allow more experienced workers to modify and update existing techniques.
An important introduction to the use of the centrifuge in the biology laboratory, Biological Centrifugation is also useful for more experienced workers. The book describes the background and the principles behind centrifugation, including sedimentation theory. The book also considers the different types of centrifuge and other centrifuge hardware available, density gradient media and gradient technology. Although aimed primarily at the novice, this title also provides information to allow more experienced workers to modify and update existing techniques.
Centrifugal Separations in Molecular and Cell Biology focuses on the application of modern centrifugation technology in molecular and cell biology, including the separation and fractionation of biological particles by centrifugation on the preparative and analytical scales. The selection first covers the principles and practices of centrifugation and the bases of centrifugal separations. Discussions focus on the basic concepts of sedimentation theory, centrifugation methods, designing centrifugation experiments, care of centrifuges and rotors, and statistical estimation of molecular parameters. The book also ponders on the practical aspects of rate-zonal centrifugation, including gradient materials, density and viscosity of glycerol solutions, and resolution and gradient shape. The publication examines fractionations in zonal rotors and the quantitative aspects of rate-zonal centrifugation. The text then reviews isopycnic centrifugation in ionic media and analytical centrifugation. Topics include separation by isopycnic banding, large-scale preparative procedures, and density-gradient solutes. The selection is a valuable reference for readers interested in centrifugation technology.