Advanced Photon Counting Techniques VI.
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Published: 2012
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DOWNLOAD EBOOKRead and Download eBook Full
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Published: 2012
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DOWNLOAD EBOOKAuthor: Mark A. Itzler
Publisher:
Published: 2012
Total Pages: 240
ISBN-13: 9780819490537
DOWNLOAD EBOOKIncludes Proceedings Vol. 7821
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DOWNLOAD EBOOKAuthor: Peter Kapusta
Publisher: Springer
Published: 2015-04-23
Total Pages: 371
ISBN-13: 3319156365
DOWNLOAD EBOOKThis volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Author: Mark A. Itzler
Publisher:
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Total Pages: 250
ISBN-13:
DOWNLOAD EBOOKAnnotation Includes Proceedings Vol. 7821.
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Published: 2018
Total Pages:
ISBN-13: 9781510618299
DOWNLOAD EBOOKAuthor: Wolfgang Becker
Publisher: Springer Science & Business Media
Published: 2005-12-19
Total Pages: 414
ISBN-13: 3540288821
DOWNLOAD EBOOKIn 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.
Author: Mark A. Itzler
Publisher:
Published: 2018
Total Pages:
ISBN-13: 9781510618305
DOWNLOAD EBOOKAuthor: Mark A. Itzler
Publisher:
Published: 2020
Total Pages:
ISBN-13: 9781510635494
DOWNLOAD EBOOKAuthor: Mark A. Itzler
Publisher: SPIE-International Society for Optical Engineering
Published: 2011
Total Pages: 306
ISBN-13: 9780819486073
DOWNLOAD EBOOKIncludes Proceedings Vol. 7821