Trends in Enzyme Histochemistry and Cytochemistry
Trends in Enzyme Histochemistry and Cytochemistry

Author: David Evered

Publisher: John Wiley & Sons

Published: 2009-09-16

Total Pages: 192

ISBN-13: 0470718242

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The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.

Enzyme Histochemistry
Enzyme Histochemistry

Author: Z. Lojda

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 350

ISBN-13: 3642672345

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During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.

Enzyme Histochemistry
Enzyme Histochemistry

Author: Cornelis J. F. van Noorden

Publisher: Oxford University Press, USA

Published: 1992

Total Pages: 136

ISBN-13:

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The functional analysis of macromolecular structures in tissues and cells has been greatly enhanced by advances in histochemistry and cytochemistry. Enzyme histochemistry is becoming particularly important as new methods succeed in demonstrating and quantifying the activity of key regulatory enzymes. The specificity, precision, and reproducibility of enzyme histochemical methods are constantly improving. This practical laboratory handbook contains a selection of the most important enzyme histochemical techniques currently available for light microscopy. The methods included here were chosen because of their reliability and specificity, and all are clearly detailed in easy-to-follow protocols. The introductory chapter provides a good theoretical background to enzyme histochemistry, and the book will be of interest to all researchers in cell biology, pathology, biochemistry, and cell physiology.

Histochemical and Immunohistochemical Techniques
Histochemical and Immunohistochemical Techniques

Author: Peter H. Bach

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 292

ISBN-13: 9401130949

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Toxicological and pharmacological effects arise when chemicals interact with biophysiological functions in discrete cell types. There is a continuing need to screen novel compounds for their potential therapeutic effects, and once these have been "discovered" to understand their molecular actions, as the basis of using such compounds safely and for rational drug design. Pharmacology now uses all of the sophisticated molecular research techniques that are available for the development of safer and more efficacious drugs. Histochemistry has been usefully applied to developing new drugs (and assessing chemical safley) and is potentially cost effective. The need to test novel substances for their potential adverse effects has raised many questions. Toxicological pathology has moved away from the cataloging of lesions towards understanding the basis of the events that underly cell injury, especially for those secondary consequences of chemical injury that lead to malignancy and chronic disease. The focal nature of toxicologic lesions de mands the use of microtechniques to provide data to help understand these questions. Histochemistry is under-utilized, but offers one of the key ap proaches necessary to address the problem of understanding interactions between a cell population and a chemical, the modulation of cellular biochem istryor the presence of a lesion in a test animal can be rationalised in terms of species differences that have no relevance to man as opposed to those that are of clinical significance or represent a warning of dire consequences to man.

Theory and Strategy in Histochemistry
Theory and Strategy in Histochemistry

Author: Hans Lyon

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 582

ISBN-13: 3642737420

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If you want practical information on how to use this book please refer to "Note to the Readers" p. VII. Histochemistry and cytochemistry are essential tools in biomedical research and routine service laboratories. Most texts on histochemistry fall into one of two categories: 1. Encyclopaedic texts covering all or nearly all information available on the whole or selected parts of histochemistry. 2. Reviews or surveys of methods found to be useful by the author(s). While the former category often appeals to the more philosophically inclined reader, direct guidance on the selection of technique may be difficult to find. In contrast, the latter category are often excellent sources for details on how to perform a particular method with a reasonable chance of success. Consideration of the exact mechanism of staining, of possible reasons for failure, and of alternative techniques are, however, frequently lacking. This book is an introduction to the scientific basis of histochemistry and is intended to provide a background for the selection and development of appro priate methods. It is not a "cook book" and readers expecting exhaustive methodological descriptions will be disappointed. Although most ofthe contributors to this book would not describe themselves as histochemists, they have all at some time found it essential to develop a basic understanding of histochemistry. This book contains the information they would have greatly appreciated ready access to at that time.

Fluorescence Microscopy
Fluorescence Microscopy

Author: F. W. D. Rost

Publisher: Cambridge University Press

Published: 1992

Total Pages: 486

ISBN-13: 9780521410885

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Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.

Techniques in Neuroanatomical Research
Techniques in Neuroanatomical Research

Author: C. Heym

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 405

ISBN-13: 3642680291

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"Anatomy is the mother of physiology" - this statement was used to characterize the evolution of physiology from anato my as an independent science in the late nineteenth century. It had particular truth for neurophysiology, which started as functional neuroanatomy based on the observation of changes in behaviour after lesions of the nervous system both in ex perimental animals and in human patients. Today, anatomy may again be considered the mother of physiology; however, the meaning of this statement is rather different from that 100 years ago: The modem mother provides a dwelling for an increasing number of children endowed with new functional capabilities. This book provides a good illustration of such se mantic metamorphosis in the case of neuroanatomy. After a long period of little progress in either macroscopic neuroanatomy or neurohistology, during which the heritage of Cajal, Golgi, and others was developed and refined to yield a functional concept of the nervous system, the past two de cades have seen tremendous progress in methods applicable to the analysis of the nervous system. The new era was heralded by the introduction of the electron microscope to investigate the nervous system. This book is an impressive witness to the more recent developments.

Bone
Bone

Author: Brian K. Hall

Publisher: CRC Press

Published: 1991-11-19

Total Pages: 366

ISBN-13: 9780849388255

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Bone: A Treatise is a series of seven volumes devoted to providing the most up-to-date, comprehensive, and authoritative coverage of all aspects of bone. Seventy-four chapters have been written by 127 respected authors, all of whom are actively engaged in basic, applied, and clinical bone research. The seven volumes in this unique series are organized thematically; each volume integrates structure, function, biochemistry, metabolism, and the molecular and clinical aspects of a particular aspect of the biology of bone. Bone-forming cells are treated in Volume I. Bone resorbing cells are covered in Volume II. Volumes III and IV examine the extracellular matrix of bone, with Volume III concentrating on the structure of bone matrix and on bone-specific proteins, while Volume IV focuses on the mineralization (calcification) of that matrix. Volume V addresses the repair and regeneration of bone. Volumes VI and VII explore bone growth and development.