This manual of essentials details the setup and operation of a polarized light microscope for microscopic particle characterization and identification using an approach to applied polarized light microscopy required for applications including industrial problem solving, contaminant identification, trace evidence analysis, and much more.
This manual of essentials details the setup and operation of a polarized light microscope for microscopic particle characterization and identification using an approach to applied polarized light microscopy required for applications including industrial problem solving, contaminant identification, trace evidence analysis, and much more.
Some of the simpler measurements of optical mineralogy are so precise and powerful that they give satisfaction to beginning students. Not long after mastering the strike and dip of rock surfaces with the Brunton compass, many geology students are able to determine precisely the identity of quartz, or the anorthite content of plagioclase, or the magne sium ratio of pyroxene with the polarizing or petrographic microscope, by means of measuring refractive index to better than one part in a thousand. Very little training and almost no theory are needed to achieve these skills. But there inevitably comes a time when theory is needed, either to get on with the art, or simply to reconstruct from first principles what is going on, when rote memory fails. In this book we hope to provide both the rote methods and the theoretical background for practitioners at all levels of experience. We draw from several careers-ours, our colleagues', and our students' -in teaching the subject at various levels of sophistication. Our book is intended to serve the needs of industrial and forensic scientists as well as petrogra phers who deal with rocks. Much of our treatment is based on new research, both in matters of presentation and in the optical determination of minerals and other materials.
This is a brief history of the development of microscopy, from the use of beads and water droplets in ancient Greece, through the simple magnifying glass, to the modern compound microscope. The technology and optical theory are developed in a straightforward manner, and this leads to a description and explanation of the most modern technologies in electron microscopy, and scanning electron microscopy as well as the new scanning probe microscopies. A series of very interesting applications of the various microscopic techniques are described. The most recent pioneering techniques in near field and confocal optical microscope technologies are described and evaluated for their future importance.
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Many of the earliest books, particularly those dating back to the 1900s and before, are now extremely scarce and increasingly expensive. We are republishing these classic works in affordable, high quality, modern editions, using the original text and artwork.
Since the first edition of this book was published in 2004, to much acclaim, the pace of innovation in the field of skin metrology has increased and various new technologies have become available. This new, revised edition reflects these advances by presenting the current theory and practice of noninvasive investigation and measurement of the skin and its appendices in health and disease. The first, extensive part of this authoritative work is devoted to the physiology and metrology of the various structural components of the skin. Skin functions and their measurement are then discussed in detail, with sections on mechanical protection, photoprotection, barrier function, immune function, thermoregulation, and sensory function. In addition, careful consideration is given to skin disease rating and skin maps, and a unique list of physical and biological constants and units is provided. Not only is this new edition the first comprehensive, practical handbook in this domain – it will also serve as a manual of skin physiology and collates anatomical, functional, and physical quantitative data that would otherwise be arduous to retrieve because of their dispersal throughout the literature. It will prove a valuable resource for dermatologists, cosmetologists, bioengineers, physiologists, pharmacists, and all others who deal with the skin in their work.
Some of the simpler measurements of optical mineralogy are so precise and powerful that they give satisfaction to beginning students. Not long after mastering the strike and dip of rock surfaces with the Brunton compass, many geology students are able to determine precisely the identity of quartz, or the anorthite content of plagioclase, or the magne sium ratio of pyroxene with the polarizing or petrographic microscope, by means of measuring refractive index to better than one part in a thousand. Very little training and almost no theory are needed to achieve these skills. But there inevitably comes a time when theory is needed, either to get on with the art, or simply to reconstruct from first principles what is going on, when rote memory fails. In this book we hope to provide both the rote methods and the theoretical background for practitioners at all levels of experience. We draw from several careers-ours, our colleagues', and our students' -in teaching the subject at various levels of sophistication. Our book is intended to serve the needs of industrial and forensic scientists as well as petrogra phers who deal with rocks. Much of our treatment is based on new research, both in matters of presentation and in the optical determination of minerals and other materials.
