With the rapid development of biotechnologies, single-cell sequencing has become an important tool for understanding the molecular mechanisms of diseases, defining cellular heterogeneities and characteristics, and identifying intercellular communications and single-cell-based biomarkers. Providing a clear overview of the clinical applications, the book presents state-of-the-art information on immune cell function, cancer progression, infection, and inflammation gained from single-cell DNA or RNA sequencing. Furthermore, it explores the role of target gene methylation in the pathogenesis of diseases, with a focus on respiratory cancer, infection and chronic diseases. As such it is a valuable resource for clinical researchers and physicians, allowing them to refresh their knowledge and improve early diagnosis and therapy for patients.
The Mouse Nervous System provides a comprehensive account of the central nervous system of the mouse. The book is aimed at molecular biologists who need a book that introduces them to the anatomy of the mouse brain and spinal cord, but also takes them into the relevant details of development and organization of the area they have chosen to study. The Mouse Nervous System offers a wealth of new information for experienced anatomists who work on mice. The book serves as a valuable resource for researchers and graduate students in neuroscience. Systematic consideration of the anatomy and connections of all regions of the brain and spinal cord by the authors of the most cited rodent brain atlases A major section (12 chapters) on functional systems related to motor control, sensation, and behavioral and emotional states A detailed analysis of gene expression during development of the forebrain by Luis Puelles, the leading researcher in this area Full coverage of the role of gene expression during development and the new field of genetic neuroanatomy using site-specific recombinases Examples of the use of mouse models in the study of neurological illness
Expert laboratory and clinical researchers from around the world review how to design and evaluate studies of tumor markers and examine their use in breast cancer patients. The authors cover both the major advances in sophisticated molecular methods and the state-of-the-art in conventional prognostic and predictive indicators. Among the topics discussed are the relevance of rigorous study design and guidelines for the validation studies of new biomarkers, gene expression profiling by tissue microarrays, adjuvant systemic therapy, and the use of estrogen, progesterone, and epidermal growth factor receptors as both prognostic and predictive indicators. Highlights include the evaluation of HER2 and EGFR family members, of p53, and of UPA/PAI-1; the detection of rare cells in blood and marrow; and the detection and analysis of soluble, circulating markers.
This text describes a system of reporting breast fine needle aspiration biopsy that uses five clearly defined categories, each described by a specific term and each with a specific risk of malignancy. The five categories are insufficient/inadequate, benign, atypical, suspicious of malignancy and malignant. Each category has a risk of malignancy and is linked to management recommendations, which include several options because it is recognized that diagnostic infrastructure, such as the availability of core needle biopsy and ultrasound guidance, vary between developed and low and middle income countries. This text includes key diagnostic cytological criteria for each of the many lesions and tumors found in the breast. The cytopathology of specific lesions is illustrated with high quality photomicrographs with clear figure descriptions. Chapters also discuss current and potential future ancillary tests, liquid based cytology, nipple cytology and management. An additional chapter provides an overview of an approach to the diagnosis of direct smears of breast fine needle aspiration biopsies. The International Academy of Cytology Yokohama System for Reporting Breast Fine Needle Aspiration Biopsy Cytopathology provides a clear logical approach to the diagnosis and categorization of breast lesions by FNAB cytology, and aims to facilitate communication with breast clinicians, further research into breast cytopathology and related molecular pathology, and improve patient care.
The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies. It also provides detailed information on individual subgroups of lymphocytes, including cell characters, function, surface markers, receptor function, intracellular signals and pathways, production of inflammatory mediators, nuclear receptors and factors, omics, sequencing, disease-specific biomarkers, bioinformatics, networks and dynamic networks, their role in disease and future prospects. Dr. Xiangdong Wang is a Professor of Medicine, Director of Shanghai Institute of Clinical Bioinformatics, Director of Fudan University Center for Clinical Bioinformatics, Director of the Biomedical Research Center of Zhongshan Hospital, Deputy Director of Shanghai Respiratory Research Institute, Shanghai, China.
Moving from a standard clinical reference Garner and Klintworth's Third Edition of Pathobiology of Ocular Disease is now modeled to be both a scientific research tool and a quick reference option for the practicing ophthalmologist, ophthalmic pathologist, optometrist and vision scientist. With the addition of ten new chapters, this edition focuses its attention on the causes and mechanisms of ocular disease, and is on the forefront of the latest discoveries, research methods, and the current on-going research in ocular disease.
The twelfth-century French poet Chrétien de Troyes is a major figure in European literature. His courtly romances fathered the Arthurian tradition and influenced countless other poets in England as well as on the continent. Yet because of the difficulty of capturing his swift-moving style in translation, English-speaking audiences are largely unfamiliar with the pleasures of reading his poems. Now, for the first time, an experienced translator of medieval verse who is himself a poet provides a translation of Chrétien’s major poem, Yvain, in verse that fully and satisfyingly captures the movement, the sense, and the spirit of the Old French original. Yvain is a courtly romance with a moral tenor; it is ironic and sometimes bawdy; the poetry is crisp and vivid. In addition, the psychological and the socio-historical perceptions of the poem are of profound literary and historical importance, for it evokes the emotions and the values of a flourishing, vibrant medieval past.
Shortlisted for the ABIA Award (Biography of the Year) 2015 A searingly honest memoir of family, cancer, love ... and unicycles by the founders of the Love your Sister charity, Connie and Samuel Johnson, that will inspire and they hope get people talking about boobs! Born a year apart, Connie and Samuel Johnson have always been close. Faced with the devastating news that they would soon be separated forever, they made a decision. After already surviving cancer twice in her young life, at 33 Connie was diagnosed with breast cancer. But this time it was a whole different ball game. This time she was told she will die, leaving behind her two sons. As a young mum faced with her own death, Connie wanted to make it all less meaningless, and she knew just the way to do it - send her brother, Sam, on a one-wheeled odyssey around Australia. The aims: to break the world record for the longest distance travelled on a unicycle. To raise $1 million for the Garvan Research Foundation. And, most importantly, to remind women to be breast aware and stop others having to say goodbye to those they love. Their message is simple: 'Don't fall into the booby trap.' Samuel has travelled through every state and ridden more than 150,000 kilometres to raise awareness and raise research dollars. But Connie had a secret fourth aim: to fix Samuel. And it worked. Sam cleared his diary, cleaned himself up and tenaciously kept his promise to his dying sister. For them the job isn't over. They are determined to raise more money for research. Connie vows to fight until her dying day and Sam says the fight will go on long after that. These two remarkable Australians share their tale, from childhood through to the finish line and beyond in this truly unique story. Part memoir, part travel diary, part conversation, Love your Sister is an inspiring and unforgettable story that shows just how far one man will go for his sister. The Johnsons' memoir is bracing and affecting. - Sunday Age, Sun Herald Part memoir, part diary, part conversation, this is an unforgettable story of how far a brother will go for his sister. - Brisbane News There are many joyous moments as brother and sister reflect, often wryly and honestly, on the power of their bond and the full catastrophe that is family life. - Sydney Morning Herald This book, like Connie and Samuel's lives, is much bigger than their experience of misfortune. - Canberra Times
I have a dog. An inconvenient dog. When I wake up, my dog is inconvenient. When I'm getting dressed, my dog is inconvenient. And when I'm making tunnels, my dog is SUPER inconvenient. But sometimes, an inconvenient dog can be big and warm and cuddly. Sometimes, an inconvenient dog can be the most comforting friend in the whole wide world.
