The Biogeochemistry of Cobalt in the Sargasso Sea
Author: Mak A. Saito
Publisher:
Published: 2001
Total Pages: 832
ISBN-13:
DOWNLOAD EBOOKProcesses that enable marine phytoplankton to acquire trace metals are fundamental to our understanding of primary productivity and global carbon cycling. This thesis explored the biogeochemistry of cobalt using analytical chemistry and physiological experiments with the dominant phytoplankton species, Prochlorococcus. A high sensitivity method for Co speciation was developed using hanging mercury drop cathodic stripping voltammetry. Dissolved Co at the Bermuda Atlantic Time Series station (BATS) in the Sargasso Sea was bound by strong organic complexes with a conditional stability constant of logK=16.3 +/- 0.9. Biweekly time series measurements of total cobalt near Bermuda from the MITESS sampler were 0-47pM throughout 1999, and averaged 20 +/- 10pM in 1999. A transect of total cobalt from BATS to American coastal waters ranged from 19-133pM and correlated negatively with salinity (r(sup 2)=0.93). Prochlorococcus strains MED4-Ax and SS120 showed an absolute requirement for Co, despite replete Zn. Co-57 uptake rates and growth rates were enhanced by additions of filtered low Co cultures, suggesting that a ligand is present that facilitates Co uptake. Co-limited Prochlorococcus cultures exhibited an increase in the fraction of cells in G2 relative to other cell cycle stages during exponential growth, and the durations of this stage increased with decreasing cobalt concentrations.
