Syntheses of Tumor Related Carbohydrate Antigens
Author: Hyunjin Kim
Publisher:
Published: 1999
Total Pages: 532
ISBN-13:
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Author: Hyunjin Kim
Publisher:
Published: 1999
Total Pages: 532
ISBN-13:
DOWNLOAD EBOOKAuthor: Adeline Miermont
Publisher:
Published: 2008
Total Pages: 356
ISBN-13:
DOWNLOAD EBOOKAuthor: Hong-Yang Chuang
Publisher: Springer
Published: 2015-04-23
Total Pages: 116
ISBN-13: 3662468484
DOWNLOAD EBOOKThis thesis focuses on the synthesis and vaccine evaluation of the prostate tumor- associated carbohydrate antigen RM2. The author first presents the use of the [1+2+3] one-pot sequential strategy to successfully synthesise the RM2 antigen and its analogues as single stereoisomers in every glycosylation step, producing good yields and stereoselectivity. He then introduces the conjugation of the synthetic RM2 antigen to the carrier protein CRM197 in an average number of 1–10 to create the prostate cancer vaccine candidate, which is combined with α-galactosylceramide C1, its analogue C34, or Alu. The results of the vaccination studies in mice are also described and indicate that the strongest anti-RM2 antigen titer is exhibited when one molecule of diphtheria toxin (DT) is conjugated with an average of 4.7 molecules of RM2 antigen (DT-RM4.7) and adjuvanted with the glycolipid C34. More importantly, the induced mouse antibodies mediate the effective complement-dependent cytotoxicity (CDC) against the prostate cancer cell line LNCap. The study presented in this thesis is the first ever to successfully synthesize this complex glycan molecule. Owing to the steric hindrance of the adjacent sialyl moiety, the introduction of two sialic acid units to the compact and rigid 3,4 di branched galactoside unit is very challenging and the β-selective and efficient glycosylation of the galactosamine moiety at the 4-position of di branched galactose is also problematic.
Author: University of Guelph. Department of Chemistry and Biochemistry
Publisher:
Published: 2009
Total Pages: 223
ISBN-13: 9780494501511
DOWNLOAD EBOOKAuthor: Christopher Arthur LeClair
Publisher:
Published: 2006
Total Pages: 364
ISBN-13:
DOWNLOAD EBOOKAuthor: Guillemineau Mickael
Publisher:
Published: 2012
Total Pages:
ISBN-13:
DOWNLOAD EBOOKAuthor: Doris Monyi Su
Publisher:
Published: 2008
Total Pages: 118
ISBN-13:
DOWNLOAD EBOOKAbstract: Globo-H is found to be highly expressed on many types of human cancer cell lines, including breast, colon, lung, ovarian and prostate cancers. As a distinct tumor-associated antigen marker, this epitope has been emerging as an important sequence for the development of anticancer vaccines. Many chemical methodologies for Globo-H hexasaccharide have been previously explored. This study demonstrates an alternative route, the solution-phase enzymatic synthesis of Globo-H hexasaccharide.
Author: University of Guelph. Department of Chemistry and Biochemistry
Publisher:
Published: 2006
Total Pages: 138
ISBN-13: 9780494232118
DOWNLOAD EBOOKAuthor: Per J. Garegg
Publisher:
Published: 1993
Total Pages: 244
ISBN-13:
DOWNLOAD EBOOKDeveloped from a symposium at the Fourth Chemical Congress of North America (202nd National Meeting of the ACS) in New York City, August 1991, chapter-papers present research on topics including how proteins recognize and bind oligosaccharides, synthesis and immunological properties of glycopeptide T-cell determinants, Vibrio cholerae polysaccharide studies, and purification of oligosaccharide antigens by weak affinity chromatography. Annotation copyright by Book News, Inc., Portland, OR
Author: University of Guelph. Department of Chemistry and Biochemistry
Publisher:
Published: 2005
Total Pages: 117
ISBN-13: 9780494058190
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