Single-Cell Genomics
Single-Cell Genomics

Author: Parwinder Kaur

Publisher: Springer

Published: 2025-06-13

Total Pages: 0

ISBN-13: 9783030409500

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Cells, the basic units of biological structure and function, vary broadly in type and state. Individual cells are the building blocks of tissues, organs, and organisms. Each tissue contains cells of many types, and cells of each type can switch among biological states. Single-cell genomics, transcriptomics and epigenomics open a whole new era with the possibility to interrogate every cell of an organism in order to decipher the important biological processes that occur within. This has emerged as a ground-breaking technology that has greatly enhanced our understanding of the complexity of gene expression dynamics at a microscopic resolution. It is anticipated that in the next 5-10 years, the wider research community will be routinely employing this powerful technology as a laboratory staple. Single-cell genomics, transcriptomics and epigenomics hold the potential to revolutionize the way we characterize complex cell assemblies and study their spatial organization, dynamics, clonal distribution, pathways, function, and crosstalks. These fascinating advances have opened up a new field of cell population genomics. Single-cell genomics, transcriptomics and epigenomics research is providing new insights into inter-cellular population genomic diversity, heterogeneity, specialization, taxonomy, spatial and temporal gene regulation, and cellular and organismal development and evolution. It is facilitating plant breeding, understanding of human disease conditions and personalized medicine. This book discusses the perspectives, progress, and promises of single-cell genomics, transcriptomics and epigenomics research and applications in addressing the above and other key biological aspects in all organisms. It establishes the current state-of-the-field and serves as the foundation for future developments in single-cell genomics, transcriptomics, and epigenomics.

Single Cell Methods
Single Cell Methods

Author: Valentina Proserpio

Publisher:

Published: 2019

Total Pages: 452

ISBN-13: 9781493992423

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This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab.

Single Cell Sequencing and Systems Immunology
Single Cell Sequencing and Systems Immunology

Author: Xiangdong Wang

Publisher: Springer

Published: 2015-03-27

Total Pages: 184

ISBN-13: 9401797536

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The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies. It also provides detailed information on individual subgroups of lymphocytes, including cell characters, function, surface markers, receptor function, intracellular signals and pathways, production of inflammatory mediators, nuclear receptors and factors, omics, sequencing, disease-specific biomarkers, bioinformatics, networks and dynamic networks, their role in disease and future prospects. Dr. Xiangdong Wang is a Professor of Medicine, Director of Shanghai Institute of Clinical Bioinformatics, Director of Fudan University Center for Clinical Bioinformatics, Director of the Biomedical Research Center of Zhongshan Hospital, Deputy Director of Shanghai Respiratory Research Institute, Shanghai, China.

Cancer Evolution
Cancer Evolution

Author: Charles Swanton

Publisher: Perspectives Cshl

Published: 2017

Total Pages: 350

ISBN-13: 9781621821434

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Tumor progression is driven by mutations that confer growth advantages to different subpopulations of cancer cells. As a tumor grows, these subpopulations expand, accumulate new mutations, and are subjected to selective pressures from the environment, including anticancer interventions. This process, termed clonal evolution, can lead to the emergence of therapy-resistant tumors and poses a major challenge for cancer eradication efforts. Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Medicine examines cancer progression as an evolutionary process and explores how this way of looking at cancer may lead to more effective strategies for managing and treating it. The contributors review efforts to characterize the subclonal architecture and dynamics of tumors, understand the roles of chromosomal instability, driver mutations, and mutation order, and determine how cancer cells respond to selective pressures imposed by anticancer agents, immune cells, and other components of the tumor microenvironment. They compare cancer evolution to organismal evolution and describe how ecological theories and mathematical models are being used to understand the complex dynamics between a tumor and its microenvironment during cancer progression. The authors also discuss improved methods to monitor tumor evolution (e.g., liquid biopsies) and the development of more effective strategies for managing and treating cancers (e.g., immunotherapies). This volume will therefore serve as a vital reference for all cancer biologists as well as anyone seeking to improve clinical outcomes for patients with cancer.

Next Steps for Functional Genomics
Next Steps for Functional Genomics

Author: National Academies of Sciences, Engineering, and Medicine

Publisher: National Academies Press

Published: 2020-12-18

Total Pages: 201

ISBN-13: 0309676738

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One of the holy grails in biology is the ability to predict functional characteristics from an organism's genetic sequence. Despite decades of research since the first sequencing of an organism in 1995, scientists still do not understand exactly how the information in genes is converted into an organism's phenotype, its physical characteristics. Functional genomics attempts to make use of the vast wealth of data from "-omics" screens and projects to describe gene and protein functions and interactions. A February 2020 workshop was held to determine research needs to advance the field of functional genomics over the next 10-20 years. Speakers and participants discussed goals, strategies, and technical needs to allow functional genomics to contribute to the advancement of basic knowledge and its applications that would benefit society. This publication summarizes the presentations and discussions from the workshop.

Mapping and Sequencing the Human Genome
Mapping and Sequencing the Human Genome

Author: National Research Council

Publisher: National Academies Press

Published: 1988-01-01

Total Pages: 128

ISBN-13: 0309038405

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There is growing enthusiasm in the scientific community about the prospect of mapping and sequencing the human genome, a monumental project that will have far-reaching consequences for medicine, biology, technology, and other fields. But how will such an effort be organized and funded? How will we develop the new technologies that are needed? What new legal, social, and ethical questions will be raised? Mapping and Sequencing the Human Genome is a blueprint for this proposed project. The authors offer a highly readable explanation of the technical aspects of genetic mapping and sequencing, and they recommend specific interim and long-range research goals, organizational strategies, and funding levels. They also outline some of the legal and social questions that might arise and urge their early consideration by policymakers.

Whole Genome Amplification
Whole Genome Amplification

Author: Simon Hughes

Publisher: Methods Express (Paperback)

Published: 2005

Total Pages: 224

ISBN-13:

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Whole genome amplification generates microgram quantities of genomic DNA starting from a sample of as little as a few femtograms and so is a vital technique when sample material is limited, as well as for high-throughput assays. Whole Genome Amplification: Methods Expressis a comprehensive up-to-date laboratory manual for this key technique. It provides detailed step-by-step protocols as well as hints and tips for success and troubleshooting, taking readers through all aspects of whole genome amplification. This book is an essential practical guide for any researcher currently using PCR for genomic amplification or who wishes to do so in future.

Introduction to Genomics
Introduction to Genomics

Author: Arthur Lesk

Publisher: Oxford University Press, USA

Published: 2012

Total Pages: 421

ISBN-13: 0199564353

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This book covers the latest techniques that enable us to study the genome in detail, the book explores what the genome tells us about life at the level of the molecule, the cell, and the organism

The Major Transitions in Evolution
The Major Transitions in Evolution

Author: John Maynard Smith

Publisher: Oxford University Press

Published: 1997-10-30

Total Pages: 361

ISBN-13: 019850294X

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During evolution there have been several major changes in the way genetic information is organized and transmitted from one generation to the next. These transitions include the origin of life itself, the first eukaryotic cells, reproduction by sexual means, the appearance of multicellular plants and animals, the emergence of cooperation and of animal societies. This is the first book to discuss all these major transitions and their implications for our understanding of evolution.Clearly written and illustrated with many original diagrams, this book will be welcomed by students and researchers in the fields of evolutionary biology, ecology, and genetics.

Topological Data Analysis for Genomics and Evolution
Topological Data Analysis for Genomics and Evolution

Author: Raúl Rabadán

Publisher: Cambridge University Press

Published: 2019-10-31

Total Pages: 521

ISBN-13: 1108753396

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Biology has entered the age of Big Data. The technical revolution has transformed the field, and extracting meaningful information from large biological data sets is now a central methodological challenge. Algebraic topology is a well-established branch of pure mathematics that studies qualitative descriptors of the shape of geometric objects. It aims to reduce questions to a comparison of algebraic invariants, such as numbers, which are typically easier to solve. Topological data analysis is a rapidly-developing subfield that leverages the tools of algebraic topology to provide robust multiscale analysis of data sets. This book introduces the central ideas and techniques of topological data analysis and its specific applications to biology, including the evolution of viruses, bacteria and humans, genomics of cancer and single cell characterization of developmental processes. Bridging two disciplines, the book is for researchers and graduate students in genomics and evolutionary biology alongside mathematicians interested in applied topology.