Purification of Biotechnological Products
Purification of Biotechnological Products

Author: Adalberto Pessoa Jr

Publisher: CRC Press

Published: 2024-05-23

Total Pages: 539

ISBN-13: 1040017665

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This outstanding text focuses on providing professionals and students working in the pharmaceutical and biotechnology field with the background necessary for developing of a product or process and with the necessary rigor required by federal regulatory agencies in the pharmaceutical industry. The material will enable teachers, lecturers and professors in biotechnology to prepare courses on basic concepts and applications for the purification of biotechnological products of industrial interest. These can be applied in practice, for example, with projects on purification development on an industrial scale or useful unit operations for the development of bioproducts of commercial interest. Features: Purification and development of new bioproducts and improvement of those being produced Provides a background and concepts on the purification of biomolecules and with an industrial perspective It allows professionals to understand the entire process of developing a biopharmaceutical or bio-food, from bench to industry in biotechnology; one of the fastest-growing sectors of the economy It promotes the dissemination of information in a didactic way which is of paramount importance for interdisciplinary fields It enables the reader to follow step-by-step stages of the development of a new biopharmaceutical, and allows the optimization of existing processes

Downstream Industrial Biotechnology
Downstream Industrial Biotechnology

Author: Michael C. Flickinger

Publisher: John Wiley & Sons

Published: 2013-07-17

Total Pages: 884

ISBN-13: 111861898X

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DOWNSTREAM INDUSTRIAL BIOTECHNOLOGY An affordable, easily accessible desk reference on biomanufacturing, focused on downstream recovery and purification Advances in the fundamental knowledge surrounding biotechnology, novel materials, and advanced engineering approaches continue to be translated into bioprocesses that bring new products to market at a significantly faster pace than most other industries. Industrial scale biotechnology and new manufacturing methods are revolutionizing medicine, environmental monitoring and remediation, consumer products, food production, agriculture, and forestry, and continue to be a major area of research. The downstream stage in industrial biotechnology refers to recovery, isolation, and purification of the microbial products from cell debris, processing medium and contaminating biomolecules from the upstream process into a finished product such as biopharmaceuticals and vaccines. Downstream process design has the greatest impact on overall biomanufacturing cost because not only does the biochemistry of different products ( e.g., peptides, proteins, hormones, antibiotics, and complex antigens) dictate different methods for the isolation and purification of these products, but contaminating byproducts can also reduce overall process yield, and may have serious consequences on clinical safety and efficacy. Therefore downstream separation scientists and engineers are continually seeking to eliminate, or combine, unit operations to minimize the number of process steps in order to maximize product recovery at a specified concentration and purity. Based on Wiley’s Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, this volume features fifty articles that provide information on down- stream recovery of cells and protein capture; process development and facility design; equipment; PAT in downstream processes; downstream cGMP operations; and regulatory compliance. It covers: Cell wall disruption and lysis Cell recovery by centrifugation and filtration Large-scale protein chromatography Scale down of biopharmaceutical purification operations Lipopolysaccharide removal Porous media in biotechnology Equipment used in industrial protein purification Affinity chromatography Antibody purification, monoclonal and polyclonal Protein aggregation, precipitation and crystallization Freeze-drying of biopharmaceuticals Biopharmaceutical facility design and validation Pharmaceutical bioburden testing Regulatory requirements Ideal for graduate and advanced undergraduate courses on biomanufacturing, biochemical engineering, biopharmaceutical facility design, biochemistry, industrial microbiology, gene expression technology, and cell culture technology, Downstream Industrial Biotechnology is also a highly recommended resource for industry professionals and libraries.

Three Phase Partitioning
Three Phase Partitioning

Author: Munishwar Nath Gupta

Publisher: Elsevier

Published: 2021-08-11

Total Pages: 346

ISBN-13: 0323859038

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Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more. - Written by researchers who have been pioneers in developing and utilizing three phase partitioning - Focuses on applications, with chapters detailing relevance to a wide variety of areas and numerous practical examples - Designed to give laboratory workers the information needed to undertake the challenge of designing successful three-phase partitioning protocols

Downstream Processing of Proteins
Downstream Processing of Proteins

Author: Mohamed A. Desai

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 233

ISBN-13: 1592590276

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Considerable effort and time is allocated to introducing cell culture and fermentation technology to undergraduate students in academia, generally through a range of courses in industrial biotechnology and related disciplines. Similarly, a large number of textbooks are available to describe the appli- tions of these technologies in industry. However, there has been a general lack of appreciation of the significant developments in downstream processing and isolation technology, the need for which is largely driven by the stringent re- latory requirements for purity and quality of injectable biopharmaceuticals. This is particularly reflected by the general absence of coverage of this s- ject in many biotechnology and related courses in educational institutions. For a considerable while I have felt that there is increasing need for an introductory text to various aspects of downstream processing, particularly with respect to the needs of the biopharmaceutical and biotechnology ind- try. Although there are numerous texts that cover various aspects of protein purification techniques in isolation, there is a need for a work that covers the broad range of isolation technology in an industrial setting. It is anticipated that Downstream Processing of Proteins: Methods and Protocols will play a small part in filling this gap and thus prove a useful contribution to the field. It is also designed to encourage educational strategists to broaden the coverage of these topics in industrial biotechnology courses by including accounts of this important and rapidly developing element of the industrial process.

Bioseparations Downstream Processing for Biotechnology
Bioseparations Downstream Processing for Biotechnology

Author: Paul A. Belter

Publisher: Wiley-Interscience

Published: 1994-10-25

Total Pages: 0

ISBN-13: 9780471121138

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Offers a concise introduction to the separation and purification of biochemicals. Bridges two scientific cultures, providing an introduction to bioseparations for scientists with no background in engineering and for engineers with little grounding in biology. The authors supplement the ideas by simple worked examples, making the techniques of bioseparations easy to learn. Discusses removal of insolubles, product isolation, purification and polishing.

Putting Biotechnology to Work
Putting Biotechnology to Work

Author: National Research Council

Publisher: National Academies Press

Published: 1992-02-01

Total Pages: 133

ISBN-13: 0309047854

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The ability of the United States to sustain a dominant global position in biotechnology lies in maintaining its primacy in basic life-science research and developing a strong resource base for bioprocess engineering and bioproduct manufacturing. This book examines the status of bioprocessing and biotechnology in the United States; current bioprocess technology, products, and opportunities; and challenges of the future and what must be done to meet those challenges. It gives recommendations for action to provide suitable incentives to establish a national program in bioprocess-engineering research, development, education, and technology transfer.

Biochemical Engineering and Biotechnology
Biochemical Engineering and Biotechnology

Author: Ghasem Najafpour

Publisher: Elsevier

Published: 2015-02-24

Total Pages: 669

ISBN-13: 0444633774

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Biochemical Engineering and Biotechnology, 2nd Edition, outlines the principles of biochemical processes and explains their use in the manufacturing of every day products. The author uses a diirect approach that should be very useful for students in following the concepts and practical applications. This book is unique in having many solved problems, case studies, examples and demonstrations of detailed experiments, with simple design equations and required calculations. - Covers major concepts of biochemical engineering and biotechnology, including applications in bioprocesses, fermentation technologies, enzymatic processes, and membrane separations, amongst others - Accessible to chemical engineering students who need to both learn, and apply, biological knowledge in engineering principals - Includes solved problems, examples, and demonstrations of detailed experiments with simple design equations and all required calculations - Offers many graphs that present actual experimental data, figures, and tables, along with explanations

Separation and Purification Technologies in Biorefineries
Separation and Purification Technologies in Biorefineries

Author: Shri Ramaswamy

Publisher: John Wiley & Sons

Published: 2013-02-04

Total Pages: 730

ISBN-13: 111849346X

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Separation and purification processes play a critical role in biorefineries and their optimal selection, design and operation to maximise product yields and improve overall process efficiency. Separations and purifications are necessary for upstream processes as well as in maximising and improving product recovery in downstream processes. These processes account for a significant fraction of the total capital and operating costs and also are highly energy intensive. Consequently, a better understanding of separation and purification processes, current and possible alternative and novel advanced methods is essential for achieving the overall techno-economic feasibility and commercial success of sustainable biorefineries. This book presents a comprehensive overview focused specifically on the present state, future challenges and opportunities for separation and purification methods and technologies in biorefineries. Topics covered include: Equilibrium Separations: Distillation, liquid-liquid extraction and supercritical fluid extraction. Affinity-Based Separations: Adsorption, ion exchange, and simulated moving bed technologies. Membrane Based Separations: Microfiltration, ultrafiltration and diafiltration, nanofiltration, membrane pervaporation, and membrane distillation. Solid-liquid Separations: Conventional filtration and solid-liquid extraction. Hybrid/Integrated Reaction-Separation Systems: Membrane bioreactors, extractive fermentation, reactive distillation and reactive absorption. For each of these processes, the fundamental principles and design aspects are presented, followed by a detailed discussion and specific examples of applications in biorefineries. Each chapter also considers the market needs, industrial challenges, future opportunities, and economic importance of the separation and purification methods. The book concludes with a series of detailed case studies including cellulosic bioethanol production, extraction of algae oil from microalgae, and production of biopolymers. Separation and Purification Technologies in Biorefineries is an essential resource for scientists and engineers, as well as researchers and academics working in the broader conventional and emerging bio-based products industry, including biomaterials, biochemicals, biofuels and bioenergy.

Industrial Biotechnology
Industrial Biotechnology

Author: Christoph Wittmann

Publisher: John Wiley & Sons

Published: 2017-03-06

Total Pages: 642

ISBN-13: 3527341811

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The latest volume in the Advanced Biotechnology series provides an overview of the main product classes and platform chemicals produced by biotechnological processes today, with applications in the food, healthcare and fine chemical industries. Alongside the production of drugs and flavors as well as amino acids, bio-based monomers and polymers and biofuels, basic insights are also given as to the biotechnological processes yielding such products and how large-scale production may be enabled and improved. Of interest to biotechnologists, bio and chemical engineers, as well as those working in the biotechnological, chemical, and food industries.

New Bioprocessing Strategies: Development and Manufacturing of Recombinant Antibodies and Proteins
New Bioprocessing Strategies: Development and Manufacturing of Recombinant Antibodies and Proteins

Author: Bob Kiss

Publisher: Springer

Published: 2018-12-06

Total Pages: 473

ISBN-13: 3319971107

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This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.