Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin

Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin

Author: N. Thajuddin

Publisher: Springer Nature

Published: 2024-01-19

Total Pages: 504

ISBN-13: 9819945143

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This protocol book provides detailed procedures for the isolation of cyanobacteria, extraction, quantification, and detection of cyanotoxins. It illustrates the sampling and processing of toxin-producing cyanobacteria in water and aquatic animal samples, detection of cyanotoxins from Anabaena, Anabaenopsis, Cylindrospermopsis, Microsystis, Microcystis, Nodularia, Nostoc, Schizotrix, Lyngbya, Raphidiopsis, Oscillatoria, Planktothrix in aquatic resources. It also covers toxicity analysis by various bioassay protocols, and in vitro and insilico analysis methods. The book also reviews the methods for cyanotoxin extraction, detection, and quantification by various tools including LC-MS/MS, HPLC, NMR, PCR, and HESI-MS/MS. A separate section is dedicated to the advanced methods in Cyanotoxin analysis including the Molecular Imprinting Method (MIM), Cellular signaling biosensor, Electrochemical sensor, Nanosensors, and screening of Polyketide synthase gene. The analysis of various toxin-producing genes like sxtA and mcy is also accounted for in this book. In a nutshell, the book gives comprehensive procedures about the basics and preliminary processes that are involved in sample collection to advanced methods incorporated into the well-explored and unexplored Cyanobacterial toxin. Consequently, this manual is useful for both beginners and advanced researchers, including postgraduate students, academicians, researchers, and scientists in the field of Cyanobacterial research. ​


Cyanobacterial Harmful Algal Blooms: State of the Science and Research Needs

Cyanobacterial Harmful Algal Blooms: State of the Science and Research Needs

Author: H. Kenneth Hudnell

Publisher: Springer Science & Business Media

Published: 2008-03-13

Total Pages: 955

ISBN-13: 0387758658

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With the ever-increasing incidence of harmful cyanobacterial algal blooms, this monograph has added urgency and will be essential reading for all sorts of researchers, from neuroscientists to cancer research specialists. The volume contains the proceedings of the 2005 International Symposium on Cyanobacterial Harmful Algal Blooms, and has been edited by H. Kenneth Hudnell, of the US Environmental Protection Agency. It contains much of the most recent research into the subject.


Protocols for Macroalgae Research

Protocols for Macroalgae Research

Author: Bénédicte Charrier

Publisher: CRC Press

Published: 2018-04-17

Total Pages: 496

ISBN-13: 1351132946

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This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments.


Methods in Cyanobacterial Research

Methods in Cyanobacterial Research

Author: Shailendra Pratap Singh

Publisher: CRC Press

Published: 2024-06-14

Total Pages: 425

ISBN-13: 1040029108

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This laboratory reference compiles the essential protocols used in cyanobacterial research. Cyanobacteria is a model system for biofuel production and sequestration of carbon dioxide to tackle global climate change due to increasing levels of greenhouse gases. Topics range from identification and growth parameters to biomolecule estimation and isolation. It also covers genome analysis and bioengineering of cyanobacteria for novel products. Key Features: Provides step-by-step procedures and troubleshooting tips Covers identification, growth parameters and pigment estimation methodologies Describes isolation, characterization and purification of microcystin, phycobiliproteins and scytonemin from cyanobacteria Discusses genomics, proteomics and bioengineering of cyanobacteria from novel products Explains sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria This collection is useful to students and researchers in life sciences. It is also meant for industry experts who are involved in the production of biofuels, biofertilizers, other value-added products, and carbon sequestration using cyanobacteria.


Water Treatment for Purification from Cyanobacteria and Cyanotoxins

Water Treatment for Purification from Cyanobacteria and Cyanotoxins

Author: Anastasia E. Hiskia

Publisher: John Wiley & Sons

Published: 2020-09-28

Total Pages: 367

ISBN-13: 111892861X

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Provides a comprehensive overview of key methods for treating water tainted by cyanobacteria and cyanotoxins Toxigenic cyanobacteria are one of the main health risks associated with water resources. Consequently, the analysis, control, and removal of cyanobacteria and cyanotoxins from water supplies is a high priority research area. This book presents a comprehensive review of the state-of-the-art research on water treatment methods for the removal of cyanobacteria, taste and odor compounds, and cyanotoxins. Starting with an introduction to the subject, Water Treatment for Purification from Cyanobacteria and Cyanotoxins offers chapters on cyanotoxins and human health, conventional physical-chemical treatment for the removal of cyanobacteria/cyanotoxins, removal of cyanobacteria and cyanotoxins by membrane processes, biological treatment for the destruction of cyanotoxins, and conventional disinfection and/or oxidation processes. Other chapters look at advanced oxidation processes, removal/destruction of taste and odour compounds, transformation products of cyanobacterial metabolites during treatment and integrated drinking water processes. Provides a comprehensive overview of key methods for treating water tainted by cyanobacteria and cyanotoxins Bridges the gap between basic knowledge of cyanobacteria/cyanotoxins and practical management guidelines Includes integrated processes case studies and real-life examples Developed within the frame of the European Cooperation in Science and Technology (COST)–funded CYANOCOST A must-have resource for every water treatment plant, Water Treatment for Purification from Cyanobacteria and Cyanotoxins is a valuable resource for all researchers in water chemistry and engineering, environmental chemistry as well as water companies and authorities, water resource engineers and managers, environmental and public health protection organizations.


Guidelines on recreational water quality. Volume 1

Guidelines on recreational water quality. Volume 1

Author: World Health Organization

Publisher: World Health Organization

Published: 2021-07-12

Total Pages: 164

ISBN-13: 9240031308

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Use of coastal, estuarine and freshwater recreational environments has significant benefits for health and well-being, including rest, relaxation, exercise, cultural and religious practices, and aesthetic pleasure, while also providing substantial local, regional and national economic benefits. These guidelines focus on water quality management for coastal and freshwater environments to protect public health. The guidelines: 1. describe the current state of knowledge about the possible adverse health impacts of various forms of water pollution; and2. set out recommendations for setting national health-based targets, conducting surveillance and risk assessments, putting in place systems to monitor and control risks, and providing timely advice to users on water safety.These guidelines are aimed at national and local authorities, and other entities with an obligation to exercise due diligence relating to the safety of recreational water sites. They may be implemented in conjunction with other measures for water safety (such as drowning prevention and sun exposure) and measures for environmental protection of recreational water use sites.