Handbook of Microscopy
Handbook of Microscopy

Author: Marcel Locquin

Publisher: Butterworth-Heinemann

Published: 2013-10-22

Total Pages: 335

ISBN-13: 148316487X

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Handbook of Microscopy is a manual that deals mainly with the basic instruments and techniques used in light microscopy and its biological applications. A large section is devoted to the study of organic matter in microfossils preserved in rocks, in view of its stratigraphic importance in mining and oil prospecting. This text is comprised of six chapters; the first of which introduces the reader to the basic principles as well as to the instruments and techniques used in light microscopy. This book also discusses the microscopes and electronic flashlights for photomicrography, along with the use of monochromatic light, stereological and physicochemical microanalysis, microanalysis by electron microscopy, and microdetermination of physical values. Attention then turns to staining and impregnation and methods of fixation, examination, cutting, and mounting. The remaining chapters focus on the microscopy of topological stains and non-specific cytological stains, with emphasis on special methods used in animal and plant histology and protistology and mycological methods in pathology. This book is written specifically for microscopists.

The Science of Laboratory Diagnosis
The Science of Laboratory Diagnosis

Author: John Crocker

Publisher: John Wiley & Sons

Published: 2005-12-17

Total Pages: 564

ISBN-13: 047085913X

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This fully revised and updated edition of The Science of Laboratory Diagnosis provides a concise description of all common laboratory tests available in medical practice with notes on their application, the accuracy of each test, the historical background to the adoption of various tests and their effectiveness in diagnosis. Well illustrated, with clear headings, tables, flow charts and pathology slides, most in full colour Provides an accessible reference book in which relevant information can be found easily Page design facilitates rapid assimilation of principles and key facts All the chapters have been updated and new material has been introduced to cover recently developed techniques, such as fluid-based cytology, telepathology and proteomics The Science of Laboratory Diagnosis, Second Edition is an essential primary reference source for everyone working in a clinical laboratory. This book is essential reading for pathologists, biomedical scientists, medical laboratory scientific officers and all clinicians involved in laboratory research. Reviews of the First Edition: "The text is concise, wide-ranging and easy to digest. The ease of extraction of the important facts make it an ideal source of information for use in a variety of situations from the postgraduate examination to the clinical directors' board meeting." BULLETIN OF THE ROYAL COLLEGE OF PATHOLOGISTS "The editors have done a marvellous job, more than fulfilling their stated aim of producing a volume describing the multidisciplinary state of modern pathology which will be of interest to a wide range of readers. ... I was particularly impressed by the many tables and flow charts, which can be used as aids to decision making." JOURNAL OF CLINICAL PATHOLOGY "This is an excellent book to dip into and get a feel for techniques used in the other disciplines of pathology." ANNALS OF CLINICAL BIOCHEMISTRY

Manual of Histological Techniques
Manual of Histological Techniques

Author: Santosh Kumar Mondal

Publisher: JP Medical Ltd

Published: 2017-01-31

Total Pages: 208

ISBN-13: 9386261197

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In this manual, author has included the molecular techniques, immunohistochemistry, cell blocks, and immunofluorescence along with the conventional techniques. For students' easy understanding; many figures, charts, diagrams and tables have been included. At the same time, the volume of the book has been restricted; so that students do not become overburdened during preparation of examination. [Ed.].

Advanced Techniques in Plant Sciences
Advanced Techniques in Plant Sciences

Author: Dr. D. A. Dhale

Publisher: Book Saga Publications

Published: 2023-05-28

Total Pages: 316

ISBN-13: 9395470429

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This book, ‘Advanced Techniques in Plant Sciences’ includes basic and advanced techniques like microscopy, polarizing microscopy, transmission and scanning electron microscopes, confocal microscopy, phase contrast microscopy, photomicrography, digital imaging, and microscopic measurements. The Spectroscopic techniques contain Colorimeter, Spectroscopic techniques, Ultraviolet-visible spectroscopy; Infrared spectroscopy, Nuclear magnetic resonance spectroscopy; Basic NMR techniques, NMR Imaging Principles, Electron Spin Resonance (ESR) Spectroscopy, Atomic Absorption Spectroscopy (AAS); Mass spectrometry, Flame photometer, etc. In histochemical studies, various methods include proteins, carbohydrates, lipids, and enzymes; how to prepare the macrolides; and the different methods of preparing the slides, which include Along with this, you will also study whole mounts. Also, about the sectioning of plant specimens, free hand, microtome, rotary microtome sectioning, types of microtome, and methods of serial sectioning. Maceration, Gel electrophoresis, PAGE, SDS-PAGE, Agarose gel electrophoresis; isoelectric focusing, and 2D electrophoresis Blotting Techniques: Principles and Techniques of Southern, Northern, and Western Blotting Techniques and Southwestern Blotting. Centrifugation techniques, Types of Centrifugation, Ultracentrifugation, Concept of Partition Coefficient; Chromatography, Techniques by chromatographic bed shape, Paper Chromatography, Double-way paper chromatography, Planar chromatography, Thin layer chromatography (TLC), High-performance liquid chromatography (HPLC)- Partition chromatography, Normal-phase chromatography, Displacement chromatography, Reversed-phase chromatography (RPC), Size-exclusion chromatography, Ion-exchange chromatography, Bioaffinity chromatography, Aqueous normal-phase chromatography, Isocratic flow, and gradient elution; High-Performance Thin-Layer Chromatography (HPTLC), Gas chromatography (GC), Flash column chromatography, Column chromatography, Ion chromatography, Gel-filtration chromatography or Size-exclusion chromatography, Pyrolysis gas chromatography, Countercurrent chromatography.

Neurohistology and Imaging Techniques
Neurohistology and Imaging Techniques

Author: Radek Pelc

Publisher: Humana

Published: 2020-06-25

Total Pages: 471

ISBN-13: 9781071604267

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This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through fluorochrome expression; fluorescent probes for amyloids; or optical clearing for ultramicroscopy of GFP-expressing tissues. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Neurohistology and Imaging Techniques is a valuable resource for both expert and novice users of major light microscopic imaging techniques, and those interested in exploring alternate imaging tools.