This book provides information on basic experiments on plant physiology and biochemistry. The contents have been divided in two parts i.e. plant physiology and biochemistry. The topics in plant physiology include photosynthesis, transpiration, pigments, respiration, seed germination and nutrient deficiency whereas biochemistry part covers primary metabolites, secondary metabolites, enzymes, vitamins and buffers. Techniques like chromatography, electrophoresis have also been discussed. Every effort has been made to make the book precise and concise. The theory and principle of each experiment has been provided in the beginning of each experiment to make it easily understandable. It is expected that the book will be useful for students studying plant physiology and biochemistry at undergraduate as well as post graduate level.
The purpose of this text is to examine the assimilation and metabolism of carbon and nitrogen in plants. These processes are dealt with in an integrative fashion assessing the physiology, biochemistry and molecular biology of each topic being discussed.
Biochemical methods are used in all branches of biological science including agriculture. Biochemical aspect is an integral part of plant physiology and this aspect is used to explain nearly all the phenomenon of physiological aspect of plant and/or crop. Technology and Methods for Biochemical Aspects of Plant Physiology is mainly intended for Post Graduate students and Researchers of Universities and of different Research Institutes. As It covers a broad range of subjects on the basic as well as the practical aspects of biochemical part of Plant Physiology, it is likely that it will be also useful for any student attending different theoretical or practical Plant Physiology as well as Biochemistry courses. The Book builds on: The theoretical principles and practical’s with the description of different biochemical estimations, and it contains detailed experimental protocol (s) to perform experiments along with a collection and description of principles. 2. Practical knowledge regarding the techniques used and methods applied to investigate the properties of macromolecules. 3. How to determine the charge of weak acids, bases and macromolecules by taking into account their chemical environment. 4. How to determine the charge of weak acids, bases and macromolecules by taking into account their chemical environment. 5. How to measure the macromolecular concentration of solutions by spectrophotometry. 6. How to design protocols for the purification of proteins from cell cultures or tissues. Book is useful for conducting practical classes of undergraduate and post graduate students in Plant Physiology, Biochemistry, Biotechnology, Microbiology, Agricultural science, Environmental science, Nutrition, Pharmaceutical science and other biology- related subjects. Technologies and methods used for biochemical basis of plant physiology such as photosynthesis, photorespiration, plant pigments, carbon and nitrogen assimilation, plant nutrients, phenols, secondary metabolites, nucleic acid and vitamins should be very useful to not only post graduate student, but to research workers also.
The book is exceptional in its organization with three major characteristics of plant system i.e. Plant Physiology, Biochemistry and Molecular Biology been provided under one canopy. Physiology, which deals with all the vital activities of a plant and also explains how it reacts to sustain in natural distress similarly within the plant, the types of physiological actions at biochemical level forming innumerable compounds through chains of biochemical reactions at various levels of plant growth and development becomes Biochemistry. However, the curiosity and thirst of knowledge of human being is endless. Man has been providing still inside up to the molecular and genetic levels to understand the nature of biochemical reactions and to control if possible up to the desired level and that is Molecular Biology. Now this is the time to elevate most relevant work of academic and applied importance out of vast research of diverse significance done in the last fifty years.
