Plant Genetic Transformation Technology
Plant Genetic Transformation Technology

Author: Wagdy Sawahel

Publisher: Daya Books

Published: 1997

Total Pages: 146

ISBN-13: 9788170351757

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The introduction of foreign genetic material into host cells is a vital step in genetic engineering. It is especially important when one considers the potential application of gene transfer systems to crop improvement with the aim of engineering specific traits into a wide variety of plants. The book is an overview of the current research into gene transfer technology and will be valuable for those, who are involved in the field of plant molecular biology, genetics, biochemistry, physiology and biotechnology. Contents Chapter 1: Genetic Transformation; History & Definition, Gene transfer systems, Natural transformation system (vector system), Direct gene transfer (vector-free systems), Genetic transformation strategy, Biological parameters, Requirements for genetic transformation, Arrangement of foreign DNA in the plant genome, Stability of the foreign gene, Modes of genetic recombination, Genetic transformation approaches, Classes of transformants, Inter-transformant variability; Chapter 2: Gene Delivery systems; Polycation-mediated transformation, Particle gun, Electroporation, Microinjection, U V laser microbeam, Electroinjection, Electrophoresis, Protoplast fusion, Macroinjection, Liposome system, Ca-DNA co-precipitation method, Silicon carbide fiber-vortex, Sonication; Chapter 3: Strategies for Improving Transformation Efficiency; Plasmid DNA, Carrier DNA, DNA repair, Transformation of synchronized protoplasts, Restriction-enzyme mediated event, Transformation booster sequence; Chapter 4: Organelle Transformation; Chapter 5: Shotgun Transformation; Plasmid rescue, Gene rescue, Promoter & enhancer rescue.

Plant Transformation Technologies
Plant Transformation Technologies

Author: Charles Neal Stewart

Publisher: John Wiley & Sons

Published: 2011-01-31

Total Pages: 314

ISBN-13: 0470958944

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Plant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies, Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies. With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.

Historical Technology Developments
Historical Technology Developments

Author: Yinghui Dan

Publisher: Bentham Science Publishers

Published: 2011

Total Pages: 154

ISBN-13: 1608052486

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"Plant transformation technology has played a critical role in advancing biotechnology and fundamental research and evolved as a science. This book describes the breakthrough technologies in all aspects of plant transformation in the last 27 years, which "

Safety of Genetically Engineered Foods
Safety of Genetically Engineered Foods

Author: National Research Council

Publisher: National Academies Press

Published: 2004-07-08

Total Pages: 254

ISBN-13: 0309166152

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Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Genetically Engineered Crops
Genetically Engineered Crops

Author: National Academies of Sciences, Engineering, and Medicine

Publisher: National Academies Press

Published: 2017-01-28

Total Pages: 607

ISBN-13: 0309437385

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Genetically engineered (GE) crops were first introduced commercially in the 1990s. After two decades of production, some groups and individuals remain critical of the technology based on their concerns about possible adverse effects on human health, the environment, and ethical considerations. At the same time, others are concerned that the technology is not reaching its potential to improve human health and the environment because of stringent regulations and reduced public funding to develop products offering more benefits to society. While the debate about these and other questions related to the genetic engineering techniques of the first 20 years goes on, emerging genetic-engineering technologies are adding new complexities to the conversation. Genetically Engineered Crops builds on previous related Academies reports published between 1987 and 2010 by undertaking a retrospective examination of the purported positive and adverse effects of GE crops and to anticipate what emerging genetic-engineering technologies hold for the future. This report indicates where there are uncertainties about the economic, agronomic, health, safety, or other impacts of GE crops and food, and makes recommendations to fill gaps in safety assessments, increase regulatory clarity, and improve innovations in and access to GE technology.

Genetic Engineering of Plants
Genetic Engineering of Plants

Author: National Research Council

Publisher: National Academies Press

Published: 1984-02-01

Total Pages: 97

ISBN-13: 0309034345

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"The book...is, in fact, a short text on the many practical problems...associated with translating the explosion in basic biotechnological research into the next Green Revolution," explains Economic Botany. The book is "a concise and accurate narrative, that also manages to be interesting and personal...a splendid little book." Biotechnology states, "Because of the clarity with which it is written, this thin volume makes a major contribution to improving public understanding of genetic engineering's potential for enlarging the world's food supply...and can be profitably read by practically anyone interested in application of molecular biology to improvement of productivity in agriculture."

Genetic Modification of Plants
Genetic Modification of Plants

Author: Frank Kempken

Publisher: Springer Science & Business Media

Published: 2009-12-15

Total Pages: 683

ISBN-13: 3642023916

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Conceived with the aim of sorting fact from fiction over genetically modified (GM) crops, this book brings together the knowledge of 30 specialists in the field of transgenic plants. It covers the generation and detection of these plants as well as the genetic traits conferred on transgenic plants. In addition, the book looks at a wide variety of crops, ornamental plants and tree species that are subject to genetic modifications, assessing the risks involved in genetic modification as well as the potential economic benefits of the technology in specific cases. The book’s structure, with fully cross-referenced chapters, gives readers a quick access to specific topics, whether that is comprehensive data on particular species of ornamentals, or coverage of the socioeconomic implications of GM technology. With an increasing demand for bioenergy, and the necessary higher yields relying on wider genetic variation, this book supplies all the technical details required to move forward to a new era in agriculture.

Transgenic Technology Based Value Addition in Plant Biotechnology
Transgenic Technology Based Value Addition in Plant Biotechnology

Author: Usha Kiran

Publisher: Academic Press

Published: 2020-06-19

Total Pages: 345

ISBN-13: 012818633X

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Transgenic Technology Based Value Addition in Plant Biotechnology discusses the principles, methodology and applications of transgenic technologies. With step-by-step methods on genome editing techniques and a range of potential applications, from improving crop yield to increasing therapeutic efficacy, this book is a one-stop reference for plant gene editing technologies. It will be of particular interest to researchers interested in plant biotechnology and plant genetics, as well as agricultural scientists and those concerned with medicinal plants. Includes step-by-step methods to assist students and researchers with genome editing and bioinformatics tools Highlights a number of applications of plant biotechnology, including how to achieve desired traits, such as improved crop yield Discusses principles, methodology and applications of transgenic technologies

Genetic Engineering of Horticultural Crops
Genetic Engineering of Horticultural Crops

Author: Gyana Ranjan Rout

Publisher: Academic Press

Published: 2018-01-08

Total Pages: 468

ISBN-13: 0128104406

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Genetic Engineering of Horticultural Crops provides key insights into commercialized crops, their improved productivity, disease and pest resistance, and enhanced nutritional or medicinal benefits. It includes insights into key technologies, such as marker traits identification and genetic traits transfer for increased productivity, examining the latest transgenic advances in a variety of crops and providing foundational information that can be applied to new areas of study. As modern biotechnology has helped to increase crop productivity by introducing novel gene(s) with high quality disease resistance and increased drought tolerance, this is an ideal resource for researchers and industry professionals. Provides examples of current technologies and methodologies, addressing abiotic and biotic stresses, pest resistance and yield improvement Presents protocols on plant genetic engineering in a variety of wide-use crops Includes biosafety rule regulation of genetically modified crops in the USA and third world countries

Transgenic Plants
Transgenic Plants

Author: Leandro Peña

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 427

ISBN-13: 1592598277

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The aim of Transgenic Plants: Methods and Protocols is to provide a source of information to guide the reader through a wide range of frequently used, broadly applicable, and easily reproducible techniques involved in the gene- tion of transgenic plants. Its step-by-step approach covers a series of methods for genetically transforming plant cells and tissues, and for recovering whole transgenic plants from them. The volume then moves on to the use of sele- able and reporter markers, positive selection, marker elimination after rec- ery of transgenic plants, and the analysis of transgene integration, expression, and localization in the plant genome. Although contributors usually refer to model plants in most chapters, the protocols described herein should be widely applicable to many plant species. The last two sections are devoted to me- ods of risk assessment and to exploring the current and future applications of transgenic technology in agriculture and its social implications in a case study. Transgenic Plants: Methods and Protocols is divided into six major s- tions plus an introduction, comprising 27 chapters. Part I, the Introduction, is a review of the past, present, and perspectives of the transgenic plants, from the discovery of Agrobacterium tumefaciens as a feasible transformation vector, to its use as a tool to study gene expression and function, and the current and possible future applications of this technology in agriculture, industry, and medicine.