OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 498: In vitro Phototoxicity - Reconstructed Human Epidermis Phototoxicity test method
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 498: In vitro Phototoxicity - Reconstructed Human Epidermis Phototoxicity test method

Author: OECD

Publisher: OECD Publishing

Published: 2023-07-04

Total Pages: 28

ISBN-13: 9264353089

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Skin phototoxicity (photoirritation) is defined as an acute toxic response elicited by topically or systemically administered photoreactive chemicals after the exposure of the skin to environmental light. The in vitro reconstructed human epidermis phototoxicity test (RhE PT) is used to identify the phototoxic potential of a test chemical after topical application in reconstructed human epidermis (RhE) tissues in the presence and absence of simulated sunlight.

OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 439: In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 439: In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method

Author: OECD

Publisher: OECD Publishing

Published: 2021-06-17

Total Pages: 28

ISBN-13: 9264242848

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This Test Guideline describes an in vitro procedure that may be used for the hazard identification of irritant chemicals (substances and mixtures) in accordance with the UN Globally Harmonized System of Classification and Labelling (GHS) Category 2.

OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 431: In vitro skin corrosion: reconstructed human epidermis (RHE) test method
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 431: In vitro skin corrosion: reconstructed human epidermis (RHE) test method

Author: OECD

Publisher: OECD Publishing

Published: 2019-06-18

Total Pages: 29

ISBN-13: 9264264612

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The test described in this Test Guideline allows the identification of corrosive chemical substances and mixtures and it enables the identification of non-corrosive substances and mixtures when supported by a weight of evidence determination using other existing information.

OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 495: Ros (Reactive Oxygen Species) Assay for Photoreactivity
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 495: Ros (Reactive Oxygen Species) Assay for Photoreactivity

Author: OECD

Publisher: OECD Publishing

Published: 2019-06-18

Total Pages: 18

ISBN-13: 9264567119

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Phototoxicity is defined as a toxic response is elicited by topically or systemically administered photoreactive chemicals after the exposure of the body to environmental light. Several classes of photoreactive chemicals could cause phototoxic reactions when activated by light at otherwise non-toxic doses. Phototoxicity can be categorized as photoirritation, photoallergy, and photogenotoxicity. The purpose of this test is to measure the phototoxicity of a chemical. The main event in any phototoxic reaction is the absorption of photons of a wavelength that induces the excitation of the chromophore. Excitation energy is often transferred to oxygen molecules, followed by the generation of ROS (reactive Oxygen Species). Measurements are performed using a spectrometer. The determination of the ROS generation from irradiated chemicals with simulated sunlight is indicative of phototoxic potential.

OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 442D: In Vitro Skin Sensitisation Assays addressing the Adverse Outcome Pathway Key Event on Keratinocyte activation
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 442D: In Vitro Skin Sensitisation Assays addressing the Adverse Outcome Pathway Key Event on Keratinocyte activation

Author: OECD

Publisher: OECD Publishing

Published: 2018-06-27

Total Pages: 67

ISBN-13: 9264229825

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This Test Guideline (TG) provides an in vitro procedure (the ARE-Nrf2 luciferase test method) used for supporting the discrimination between skin sensitisers and non-sensitisers in accordance with the UN GHS.