This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens.
Methicillin-resistant Staphylococcus aureus (MRSA) emerged as a clinically relevant human pathogen more than five decades ago. The virulent bacterium was first detected in hospitals and other health care facilities where vulnerable hosts, frequent exposure to the selective pressure of intensive antimicrobial therapy, and the necessity for invasive procedures created a favorable environment for dissemination. MRSA emerged as an important cause of healthcare-associated infections, particularly central line-associated bloodstream infection, ventilator-associated pneumonia, and surgical site infection (SSI). Despite the adoption of infection-control measures, the incidence of MRSA infection at most U.S. hospitals steadily increased for many years, but it is now decreasing. While the decrease in the incidence of MRSA infection may be due to efforts to screen for MRSA carriage, it may also be due to secular trends (such as efforts to improve patient safety) and to confounders (such as efforts to improve the appropriate use of antibiotics and to decrease healthcare-associated infections in general, including catheter-associated bloodstream infection, ventilator-associated pneumonia, and SSI). A number of analyses suggest that MRSA infections are associated with increased mortality and cost of care when compared with those due to strains that are susceptible to methicillin. Even the availability of newer pharmaceutical agents with specific activity against MRSA has not ameliorated the challenge of caring for patients with MRSA. The widespread use of these agents has been limited, in part due to toxicity, cost, and uncertainty as to optimal indications. The management and control of MRSA have been further complicated by dramatic changes in the epidemiology of transmission and infection observed over the past two decades. Specifically, S. aureus strains resistant to methicillin, once exclusively linked to hospital care, have increasingly been detected among patients in the community who lack conventional risk factors for MRSA infection. Community-acquired MRSA has been linked to outbreaks of infection in hospitals and health care facilities. Conventional strategies for the control of MRSA have focused on the prevention of spread from patient to patient. The effectiveness of hand hygiene in preventing the spread of MRSA has been demonstrated in observational studies in which hand hygiene promotion campaigns were associated with subsequent reductions in the incidence of MRSA among hospitalized patients. While hand hygiene remains important in the effort to control MRSA transmission, the continued spread of the pathogen after its initial introduction in most facilities has prompted efforts to identify additional strategies. The use of contact isolation-including the donning of gowns and gloves when interacting with patients colonized or infected with MRSA and the assignment of such patients to single rooms or to a room with a group of affected patients-has been widely promoted and adopted. Such isolation precautions now are the centerpiece of most authoritative guidelines for MRSA control. Despite the broad consensus associated with the use of contact isolation for MRSA prevention, the specific evidence in support of this practice remains limited and indirect. The objective of this review was to synthesize comparative studies that examined the benefits or harms of screening for MRSA carriage in the inpatient or outpatient settings. The review examined MRSA-screening strategies applied to all hospitalized or ambulatory patients, as well as screening strategies applied to selected inpatient or outpatient populations, and compared them with no screening or with screening of selected patient populations. The review evaluated MRSA-screening strategies that included screening with or without isolation and with or without attempted eradication/decolonization.
LURKING in our homes, hospitals, schools, and farms is a terrifying pathogen that is evolving faster than the medical community can track it or drug developers can create antibiotics to quell it. That pathogen is MRSA—methicillin-resistant Staphyloccocus aureus—and Superbug is the first book to tell the story of its shocking spread and the alarming danger it poses to us all. Doctors long thought that MRSA was confined to hospitals and clinics, infecting almost exclusively those who were either already ill or old. But through remarkable reporting, including hundreds of interviews with the leading researchers and doctors tracking the deadly bacterium, acclaimed science journalist Maryn McKenna reveals the hidden history of MRSA’s relentless advance—how it has overwhelmed hospitals, assaulted families, and infiltrated agriculture and livestock, moving inexorably into the food chain. Taking readers into the medical centers where frustrated physicians must discard drug after drug as they struggle to keep patients alive, she discloses an explosion of cases that demonstrate how MRSA is growing more virulent, while evolving resistance to antibiotics with astonishing speed. It may infect us at any time, no matter how healthy we are; it is carried by a stunning number of our household pets; and it has been detected in food animals from cows to chickens to pigs. With the sensitivity of a novelist, McKenna portrays the emotional and financial devastation endured by MRSA’s victims, vividly describing the many stealthy ways in which the pathogen overtakes the body and the shock and grief of parents whose healthy children were felled by infection in just hours. Through dogged detective work, she discloses the unheard warnings that predicted the current crisis and lays bare the flaws that have allowed MRSA to rage out of control: misplaced government spending, inadequate public health surveillance, misguided agricultural practices, and vast overuse of the few precious drugs we have left. Empowering readers with the knowledge they need for self-defense, Superbug sounds an alarm: MRSA has evolved into a global emergency that touches almost every aspect of modern life. It is, as one deeply concerned researcher tells McKenna, "the biggest thing since AIDS."
Find fast answers to inform your daily diagnosis and treatment decisions! Ferri’s Clinical Advisor 2021 uses the popular "5 books in 1" format to deliver vast amounts of information in a clinically relevant, user-friendly manner. This bestselling reference has been significantly updated to provide you with easy access to answers on 1,000 common medical conditions, including diseases and disorders, differential diagnoses, clinical algorithms, laboratory tests, and clinical practice guidelines—all carefully reviewed by experts in key clinical fields. Extensive algorithms, along with hundreds of new figures and tables, ensure that you stay current with today's medical practice. Contains significant updates throughout, covering all aspects of current diagnosis and treatment. Features 27 all-new topics including chronic rhinosinusitis, subclinical brain infarction, reflux-cough syndrome, radiation pneumonitis, catatonia, end-stage renal disease, and genitourinary syndrome of menopause, among others. Includes new appendices covering common herbs in integrated medicine and herbal activities against pain and chronic diseases; palliative care; and preoperative evaluation. Offers online access to Patient Teaching Guides in both English and Spanish.
Responding to the growing concern about the increased frequency and resistance of MRSA infections, this invaluable source analyzes the latest developments in the identification, assessment, and management of methicillin-resistant Staphylococcus aureus (MRSA) by leading authorities on the topic.
Staphylococci remain the most important cause of hospital-acquiredinfections in the U.S. and MRSA has become the most common cause ofskin and soft tissue infection in many parts of the world. There is now a much greater understanding of the physiology andevolution of the staphylococci and this new edition reflects therapid advancements in knowledge about this pathogen and provides acomprehensive review from both clinical and basic scienceperspectives. The first section addresses the basic biology of thestaphylococci, their molecular genetics, host defenses and hostevasion, virulence determinants, mechanisms of antibioticresistance, and laboratory techniques. The second section dealswith epidemiology, and the third section provides an overview ofthe varied clinical manifestations of human staphylococcalinfections. The fourth section covers prevention and treatment ofthese often life-threatening infections. Written by experts from around the globe, this book is essentialreading for all clinicians and basic scientists studying thestaphylococci.
Staphylococcus was first recognized as a human pathogen in 1880 and was named for its grape cluster-like appearance. In 1884, Staphylococcus aureus was identified and named for its vibrant golden color, which was later found to be the result of golden toxin production. Here, experts examine in-depth patterns of S. aureus colonization and exposures in humans, mammals, and birds that have led to the development of various clinical diseases. The mode of transmission of S. aureus and different methods for its detection in different samples are defined. Conventional antibiotic options to treat this aggressive, multifaceted, and readily adaptable pathogen are becoming limited. Alternative, novel chemotherapeutics to target S. aureus are discussed in the pages within, including herbal medicines, bee products, and modes of delivery.
Die erste umfassende und wegweisende Publikation zur Handhygiene, eines der grundlegendsten und wichtigsten Themen bei der Infektionsbekämpfung und Patientensicherheit. Für alle medizinischen Berufe ist dieses Handbuch zur Handhygiene ein wichtiges Referenzwerk, geschrieben von weltweit führenden Wissenschaftlern und Klinikern. - Geschrieben von weltweit führenden Experten des Fachgebiets. - Berücksichtigt umfassend die Richtlinien und Vorschriften der Weltgesundheitsorganisation (WHO). - Behandelt das Thema Handhygiene aus globaler Sicht, relevant für Industrie- und Entwicklungsländer. - Erörtert grundlegende sowie hochkomplexe klinische Anwendungen der Handhygiene. - Beinhaltet neue, ungewöhnliche Aspekte und Fragestellungen, wie religiöse und kulturelle Aspekte und die Einbeziehung der Patienten. - Bietet Leitlinien für nationale und weltweite Hygienekampagnen, für jeden Einzelnen, für Institutionen und Organisationen.
"This compilation provides a compact overview of the feasibility and clinical impact of novel therapies for methicillin-resistant Staphylococcus aureus (MRSA), with a focus on monoclonal antibodies, vaccines, bacteriophages, liposomes and nanotechnology, photodynamic therapy, homeopathy and botanical medicine. The authors also explore new therapeutic approaches that demonstrate efficacy in scientific research and generate interest in the medical area. Treating nosocomial MRSA infections is challenging due to inadequacies in current therapeutic options and underlying comorbidities in the patients. As such, an overview of the signs, symptoms and treatment options for clinically significant MRSA infections is presented. In closing, the authors examine the occurrence of MRSA in healthcare settings, as well as community-based infections"--
Staphylococcus aureus is resident in skin and nasal membranes with a formidable pathogenic potential to cause a variety of community and hospital acquired infections. This authoritative survey covers the impact of genome sequences into our understanding of MRSA and MSSA pathogenesis. World specialists from America, Germany, Japan, Sweden and the UK contribute knowledge and experience which will be welcome to researchers, practitioners and postgraduate students of immunology, microbiology, bacteriology and pharmacology worldwide. - An authoritative survey that covers the impact of genome sequences into the understanding of MRSA and MSSA pathogenesis - World specialists from America, Germany, Japan, Sweden and the UK contribute knowledge and experience
