Modern methods in protein chemistry

Modern methods in protein chemistry

Author: Harald Tschesche

Publisher: Walter de Gruyter GmbH & Co KG

Published: 2019-11-05

Total Pages: 476

ISBN-13: 3111439844

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Modern Protein Chemistry

Modern Protein Chemistry

Author: Gary C. Howard

Publisher: CRC Press

Published: 2001-09-12

Total Pages: 272

ISBN-13: 1420036521

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In recent years, interest in proteins has surged. This resurgence has been driven by the expansion of the post-genomic era when structural genomics and proteomics require new techniques in protein chemistry and new applications of older techniques. Protein chemistry methods are used by nearly every discipline of biomedical research. Many techniques


Methods in Protein Biochemistry

Methods in Protein Biochemistry

Author: Harald Tschesche

Publisher: Walter de Gruyter

Published: 2011-12-23

Total Pages: 379

ISBN-13: 3110252368

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This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.


Protein Chemistry

Protein Chemistry

Author: Lars Backman

Publisher: Walter de Gruyter GmbH & Co KG

Published: 2019-12-02

Total Pages: 339

ISBN-13: 3110566281

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This class-tested textbook gives an overview of the structure and functions of proteins and explains how amino acids form a defined structural entity with specific properties. The authors also introduce modern methods for purification and separation of proteins as well as different techniques for analyzing their structural and functional properties. A separate part of the book is devoted to enzymes and kinetics of enzymatic reactions.


Advanced Methods in Protein Microsequence Analysis

Advanced Methods in Protein Microsequence Analysis

Author: Brigitte Wittmann-Liebold

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 427

ISBN-13: 3642715346

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Much of the recent spectacular progress in the biological sciences can be at tributed ot the ability to isolate, analyze, and structurally characterize proteins and peptides which are present in cells and cellular organelles in only very small amounts. Recent advances in protein chemistry and in particular the application of new micromethods have led to fruitful advances in the understanding of basic cellular processes. Areas where protein-chemical studies have resulted in interest ing discoveries include the peptide hormones and their release factors, growth factors and oncogenes, bioenergetics, proton pumps and ion pumps and chan nels, topogenesis and protein secretion, molecular virology and immunology, membrane protein analysis, and receptor research. In fact, the key methods are now on hand to unravel many of the major outstanding problems of molecular biology and in particular questions of fundamental interest which relate to devel opmental biology and specificity in cell-cell interaction. In this volume we have assembled descriptions of procedures which have re cently been shown to be efficaceous for the isolation, purification, and chemical characterization of proteins and peptides that are only available in minute amounts. Emphasis is placed on well-established micromethods which have been tested and found useful in many laboratories by experienced investigators. The chapters are written by specialists, and describe a range of sensitive techniques which can be used by researchers working in laboratories with only modest resources and equipment.


Modern Methods in Forest Genetics

Modern Methods in Forest Genetics

Author: J.P. Miksche

Publisher: Springer Science & Business Media

Published: 2013-03-09

Total Pages: 303

ISBN-13: 3662125234

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The present volume contains papers developed from courses given at the International Union of Forest Research Organizations (IUFRO) Bio chemical Genetics Workshop (Working Party S.04-5) held at the Univer sity of Gottingen, Germany on July 5 through 28, 1973. The workshop was organized by Professor Robert G. Stanley and was held in memory of Professor Klaus Stern. Unfortunately, both met with untimely deaths. Professor Stanley was also instrumental in initiating the process of having the workshop proceedings published. I was asked by the workshop participants to complete this task, and I wish to acknowledge their cooperation, advice and encouragement. In addition to the courses and subsequent papers resulting from the above workshop, we have included some papers by colleagues who were unable to attend the meeting. The contents of this text may, there fore, be considered a working-manual of generally "modern" techniques that are applicable to forest genetics and breeding programs. The chapters are placed in five major categories. The first three categories follow according to classes of chemical constituents in herent to plants which are nucleic acids (DNA, RNA) , primary gene products (amino acids, proteins and enzymes) and primary and secon dary metabolites (carbohydrate polymers, resins, phenolics, pigments, etc.). The fourth category is concerned with the interaction of en vironment and gene systems. Indirect selection, crossing and proto plasmic and flowering manipulation are factors covered in the fifth category.


Methods in Protein Sequence Analysis

Methods in Protein Sequence Analysis

Author: Brigitte Wittmann-Liebold

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 608

ISBN-13: 3642738346

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"Methods in Protein Sequence Analysis - 1988" - contains selected contributions on modern protein- analytical techniques as presented by speakers at the Seventh International Conference on "Methods in Protein Sequence Analysis", held from July 3rd to July 8th, 1988 in Berlin. The book contains information on new methodologies for sensitive amino acid analysis, N- and C-terminal sequence analysis, and protein and peptide purification. In addition recent mass spectrometric approaches are described, as an alter native technique to the common stepwise degradative sequence analysis of polypeptides by the Edman method. The book presents new possibilities in the design of sequencers and sophisticated equipment for the structural analysis of peptides and proteins. It describes practical approaches for the investigation of protein domains and protein complexes, and contains review chapters on the crystallization of cell organelles as well as on recent theoretical aspects of protein folding mechanisms. The nature of protein folding is not yet understood, but further advances in this area would greatly enhance our present knowledge of protein structure and function. Further, the book gives examples of the application of gene technology to protein characterization and to the design of new proteins. This enables new studies on the structure and function of proteins to be made, and opens up efficient approaches to the design of drugs.


Techniques in Protein Chemistry

Techniques in Protein Chemistry

Author: Choh Hao Li

Publisher: Elsevier

Published: 2013-10-24

Total Pages: 285

ISBN-13: 0323162401

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Hormonal Proteins and Peptides: Techniques in Protein Chemistry, Volume IX presents the various methods in protein chemistry and discusses their use for hormonal protein investigations. This book examines the approach to investigating biologically active peptides, which is based on a combination of chromatographic and fluorometric procedures. Organized into five chapters, this volume starts with an overview of the fluorometric methods for analysis of peptides and proteins with focus on their use in high performance liquid chromatography. This text then presents a thorough discussion on partition chromatography of proteins and peptides. Other chapters consider a comprehensive account of absorption and fluorescence techniques for the structural analysis of proteins. This book presents as well a comprehensive discussion on optical rotatory dispersion and circular dichroism methods and their application for the study of protein conformation. The final chapter deals with the historical aspects of fetuin and ultracentrifuge. This book is a valuable resource for chemists, biologists, endocrinologists, and physicians.