Microvascular Corrosion Casting in Scanning Electron Microscopy

Microvascular Corrosion Casting in Scanning Electron Microscopy

Author: S.H. Aharinejad

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 518

ISBN-13: 3709192307

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Several methods have been used to demonstrate the vasculature of different organs in man and other species. Many attempts to evaluate the precise microangioarchitecture of organ systems remained unproductive, others were controversial. The development of electron microscope in thirties opend new perspectives in researching microvascular systems. Transmission electron microscopy provided a two-dimensional view on microcirculatory system at higher magnifications, however, its standardization was delayed unnecessarily. The use of methyl methacrylate and related compounds for obtaining replicas of vascular beds, and their study in scanning electron microscope opened a new window in micromorphological research. For the first time, a three-dimensional image analysis of the vascular system was possible. The microvascular corrosion casting method has meanwhile attracted the interest of many contemporary scientists. Its application to medical and biological problems justify it to be used as a routine method for microvascular investigations. The first investigators who used this method, focused either on methodological details or they dealt with the normal microanatomy of organs. The advantages of this method in demonstrating pathological microvascular patterns are also evident.


Scanning Electron Microscopy for the Life Sciences

Scanning Electron Microscopy for the Life Sciences

Author: Heide Schatten

Publisher: Cambridge University Press

Published: 2013

Total Pages: 275

ISBN-13: 0521195993

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A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.


Scanning Electron Microscopy of Vascular Casts: Methods and Applications

Scanning Electron Microscopy of Vascular Casts: Methods and Applications

Author: P. Motta

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 385

ISBN-13: 1461534887

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Recently, attention has been called to the role that microvascular organization plays in the functional morphology of all organs and tissues, both in normal and pathological conditions. Since its development by Murakami, the corrosion cast method for scanning electron microscopy has come to be considered one of the most efficient means in clarifying the three-dimensional features of the microcirculation of organs and tissues. Scanning Electron Microscopy of Vascular Casts: Methods and Applications was planned to supply fundamental and new information regarding microcirculation studies to general biologists, anatomists, pathologists and clinicians. The contributions to this volume, contain original findings and excellent electron micrographs obtained by using recently improved corrosion cast methods. The rich variety of papers in this book will be useful to many, and will provide both the basic and clinically oriented readers with good ideas, suggestions, and original and worthwhile information.


Handbook of Vascular Biology Techniques

Handbook of Vascular Biology Techniques

Author: Mark Slevin

Publisher: Springer

Published: 2015-03-27

Total Pages: 466

ISBN-13: 9401797161

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A wide range of research methods for the study of vascular development, from basic laboratory protocols to advanced technologies used in clinical practice, are covered in this work. A range of methodologies such as molecular imaging platforms and signalling analysis, along with tumour models are collated here. Four sections explore in vitro techniques, in vivo and ex vivo manipulations, imaging and histological analysis and other novel techniques in vascular biology. Readers will discover basic methodologies used for analysis of endothelial cell growth in vitro, including co-culture models of vessel formation. Authors also explore isolation and purification of cells and methods for analysis of data and visualization of localized vasculature with modern imaging platforms. Both animal models and human disease are covered in this work. Each chapter contains helpful sections on trouble shooting, additional notes and links, supporting the reader to carry out protocols. This book will appeal to students, researchers and medical professionals working in all vascular-linked fields such as cardio- and cerebrovascular, cancer and dementia.


Color Atlas of Adult Xenopus laevis

Color Atlas of Adult Xenopus laevis

Author: Alois Lametschwandtner

Publisher: Springer Nature

Published: 2022-11-18

Total Pages: 293

ISBN-13: 3031051106

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This atlas offers stunning color electron scanning micrographs and exceptional light microscopy pictures of capillaries, vessels and diverse histomorphological tissues and organs of Xenopus laevis. The model organisms Xenopus laevis serves to study basic biological questions related to growth, differentiation, maturation, and regression of cells, tissues and organs. Xenopus and human genomes have long stretches of gene collinearity, and 79% of identified human disease genes have a verified ortholog in Xenopus. Thus, this atlas will be a powerful tool for anatomists, morphologists, histologists and physiologists interested in normal and pathologically altered organs and tissue; and to all researchers, who wish to learn more about the microvascular anatomy of this vertebrate model organism.


Electron Microscopy of the Lung

Electron Microscopy of the Lung

Author: D Schrafnagel

Publisher: CRC Press

Published: 1990-05-23

Total Pages: 646

ISBN-13: 9780824783198

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Documents the enormous contribution electron microscopy has made to the study of lung biology, describing new analytical instruments, recent technological developments, and future avenues of research. Illustrated with 290 micrographs of normal and abnormal lung, rare tumors, and other features of lu


Angiogenesis

Angiogenesis

Author: Michael E. Maragoudakis

Publisher: Springer Science & Business Media

Published: 2013-11-21

Total Pages: 552

ISBN-13: 1475791852

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Interest in angiogenesis research remains strong in recent years and exciting new discoveries, about modulators of angiogenesis, their receptors, the transduction mechanisms and the angiogenic genes involved, have contributed to our present day understanding of this complex process. This knowledge has provided the basis and broadened the scope of angiogenesis - based therapy in oncology and many other clinical conditions. This monograph contains the contributions to the NATO Advanced Study Institute on "Angiogenesis: Models, Modulators and Clinical Applications", which was held in Rhodes, Greece, from June 20-30, 1997. This was the fourth of a series of NATO supported international meetings on Angiogenesis aiming to bring together basic scientists with clinicians to exchange ideas, disseminate new knowledge and discuss the present status and potential new directions in this fast moving area of biomedical research. The International Organising Committee that included Drs. E. Dejana, C Haudenschild, M. Hackel, H. Kleinman, P. Lelkes, M. Presta, P. Polverini, D. Thompson, has provided invaluable help with their insightful suggestions in the formulation of the scientific program for which I am grateful. I wish to thank all the participants for their enthusiastic participation and their complimentary comments on the success of the conference.


Ultrastructure of the Digestive Tract

Ultrastructure of the Digestive Tract

Author: P. Motta

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 266

ISBN-13: 1461320712

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When established four years ago, the scope of this international series in electron microscopy essentially was to provide an opportunity for the pUblication of selected review contributions by specialists in ultrastructural research. Previous volumes presented over the last three years have focused on special topics of present interest in ~'ontemporary biomedicine such as endocrine cells, reproduction, and connective tissues. In these fielCls, in fact, integrated methods of electron microscopy have contributed much to generate new ideas and concepts of general value in both basic and clinical applications. The Ultrastructure of the Digestive Tract basically follows the same guidelines and style of the other books in the series and is an invited collection of selected contributions of authors from various laboratories active in the field of electron microscopy. Therefore, although the various chapters consist of individual topics, they nevertheless should be considered as interrelated contributions of specific subjects in the field. The idea was to have critical reviews of aspects previously published elsewhere by experts in the field who, as a rule, include other relevant information in their articles in order to update and enrich the subject. This book contains fifteen chapters by renowned electron microscopists. Each chapter, according to the policy of the editors, reviews a particular topic in great detail, providing updated information, study methods and results, authors' ideas on future investigative approaches, and possible guidelines for forthcoming work. We hope that this book will be useful to cell biologists, morphologists, physiologists, and pathologists.


Basic, Clinical, and Surgical Nephrology

Basic, Clinical, and Surgical Nephrology

Author: L.J. Didio

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 257

ISBN-13: 1461325757

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This unique volume may very well foreshadow the treatment of renal disease in the twenty-first century. The editors have obviously compiled and reviewed the current clinical problems in which the kidney plays a major role. They then selected as topics for chapters those in which recent scientific investigations have added significant new data. The investigators themselves or their peers have been persuaded to produce a summary of current concepts of renal structure and function for each topic. The result is a volume which will be as invaluable as a clinical guide on the laboratory bench as it will be a reference for the clinician seeking guidance to rational therapy at the bedside. The strength of the volume lies in the incorporation of those data on renal cellular structure and function which hold the key to the etiology of the majority of renal diseases we now call 'end-stage'. Fully, two-thirds of the volume is devoted to current concepts of renal function and related subcellular structure of various renal tissues. The illustrations, correlations, and explanations are superbly presented in much detail and with an obvious effort to fill out the current knowledge of each subject. We may anticipate this book will remain a valuable reference for many years to come.