Methods in Molecular Biology and Protein Chemistry
Methods in Molecular Biology and Protein Chemistry

Author: Brenda D. Spangler

Publisher: Wiley

Published: 2002-06-14

Total Pages: 232

ISBN-13: 9780470843604

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Ein praxisorientierter Leitfaden für Studenten im Hauptstudium. Vermittelt werden aktuelle Techniken der Molekularbiologie und Biochemie als fester Bestandteil eines Forschungsprojektes. Auf diese Weise kann der Lernende die Auswirkung der Gentechnik anhand der praktischen Anwendung von nassanalytischen biochemischen Verfahren erkennen. Mit Computer-Tutorials für Sequenzierung, Genomik und Molekülmodellierung. Mit Tipps und Hinweisen zur Sichtung der Primärliteratur, zu mündlichen Berichten, zur Protokollierung des Labortagebuchs und zum Verfassen von Berichten. Mit einer Liste von amerikanischen und europäischen kommerziellen Anbietern von Reagenzien. Verständlich und anschaulich geschrieben. Ein unverzichtbares Lehrbuch für fortgeschrittene Studenten und Doktoranden. "Methods in Molecular Biology" ist jedoch NICHT geeignet für aktive Forscher auf der Suche nach den neuesten hochdifferenzierten Verfahren.

Methods in Protein Biochemistry
Methods in Protein Biochemistry

Author: Harald Tschesche

Publisher: Walter de Gruyter

Published: 2011-12-23

Total Pages: 379

ISBN-13: 3110252368

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This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.

Advances in Protein Molecular and Structural Biology Methods
Advances in Protein Molecular and Structural Biology Methods

Author: Timir Tripathi

Publisher: Academic Press

Published: 2022-01-14

Total Pages: 716

ISBN-13: 0323902650

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Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. - Presents a thorough overview of the latest and emerging methods and technologies for protein study - Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy - Includes computational and machine learning methods - Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins

Protein NMR Techniques
Protein NMR Techniques

Author: Alexander Shekhtman

Publisher: Humana Press

Published: 2016-05-01

Total Pages: 534

ISBN-13: 9781493961955

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In its expanded third edition, this Methods in Molecular Biology volume offers techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing, materials lists, step-by-step protocols, troubleshooting tips and more."

Protein Purification Protocols
Protein Purification Protocols

Author: Paul Cutler

Publisher: Springer Science & Business Media

Published: 2008-02-02

Total Pages: 474

ISBN-13: 159259655X

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The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.

The Protein Protocols Handbook
The Protein Protocols Handbook

Author: John M. Walker

Publisher: Springer Science & Business Media

Published: 2002

Total Pages: 1446

ISBN-13: 0896039404

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The authors are commonly the techniques" originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.

Chemical Biology
Chemical Biology

Author: Jonathan E. Hempel

Publisher: Humana

Published: 2015-01-24

Total Pages: 0

ISBN-13: 9781493922680

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This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organizes into four parts that focus on platforms for molecular discovery in in vitro cellular systems, in vivo chemical genetic screening protocols, and methods used to discover functional protein targets. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and informative, Chemical Biology: Methods and Protocols seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge.

Protein Engineering Protocols
Protein Engineering Protocols

Author: Kristian Müller

Publisher: Springer Science & Business Media

Published: 2007-10-26

Total Pages: 318

ISBN-13: 1597451878

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Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.

Molecular Biology Techniques
Molecular Biology Techniques

Author: Heather B. Miller

Publisher: Academic Press

Published: 2011-10-18

Total Pages: 227

ISBN-13: 0123855454

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions