Membrane Fusion Techniques
Membrane Fusion Techniques

Author: Nejat Düzgüneş

Publisher: Gulf Professional Publishing

Published: 1993

Total Pages: 502

ISBN-13: 9780121821227

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The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. * * Major topics covered include: * Cell-cell fusion mediated by viruses and viral proteins * Conformational changes of proteins during membrane fusion * Membrane fusion during exocytosis * Intracellular membrane fusion * Membrane fusion in fertilization * Introduction of macromolecules into cells by membrane fusion * Protoplast fusion.

Guide to Techniques in Mouse Development, Part A
Guide to Techniques in Mouse Development, Part A

Author:

Publisher: Academic Press

Published: 2010-08-04

Total Pages: 539

ISBN-13: 0080887783

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Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. - Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Covers new technologies and methodologies, including: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals - use of gene-trap mutagenesis - analysis of allele-specific expresion - use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics

Two-Component Signaling Systems, Part A
Two-Component Signaling Systems, Part A

Author:

Publisher: Elsevier

Published: 2011-09-21

Total Pages: 588

ISBN-13: 0080548717

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Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections include: Computational Analyses of Sequences and Sequence Alignments Biochemical and Genetic Assays of Individual Components of Signaling Systems Physiological Assays and Readouts - Presents detailed protocols - Includes troubleshooting tips

Biothermodynamics Part A
Biothermodynamics Part A

Author: Michael L. Johnson

Publisher: Academic Press

Published: 2009-03-14

Total Pages: 519

ISBN-13: 0080923402

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In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. The use of thermodynamics in biological research is used as an "energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance? As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well.

Liposomes, Part A
Liposomes, Part A

Author: Nejat Duzgunes

Publisher: Elsevier

Published: 2003-11-11

Total Pages: 345

ISBN-13: 008049708X

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Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. - Methods in Liposome Preparation - Physiochemical Characterization of Liposomes

Molecular Mechanisms of Membrane Fusion
Molecular Mechanisms of Membrane Fusion

Author: Shinpei Ohki

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 583

ISBN-13: 1461316596

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Membrane fusion is an important molecular event which plays a pivotal role in many dynamic cellular processes, such as exocytosis, endocytosis, membrane biogenesis, fertilization, etc. While many reports on the physico-chem1cal process involved in membrane fusion have appeared in the literature and much information has accumulated, there has been no comprehensiv~ meeting of workers in the field. A recent symposium which took place at the Center for Tomorrow, State University of New York at Buffalo, New York, April 27-29, 1987, was the first meeting of its kind to specifically address the molecular mechanisms of membrane fusion. The Symposium consisted of oral, workshop and poster presentation sessions. The papers presented in the oral and workshop sessions are collected here and arranged approximately in the order presented at the Symposium. These papers are the most up-to-date and representative work at the for front of each aspect of membrane fusion. Although the readers may find some differences in interpretations regarding the molecular mechanisms of membrane fusion, there is an over all concensus that increased hydrophobicity and dehydration of the membrane surface are essential physico-chemical factors for membrane fusion to occur. We trust that these papers will contribute to your further understanding of the mechanisms of membrane fusion.

Computer Methods Part A
Computer Methods Part A

Author:

Publisher: Academic Press

Published: 2009-03-10

Total Pages: 491

ISBN-13: 0080923291

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The combination of faster, more advanced computers and more quantitatively oriented biomedical researchers has recently yielded new and more precise methods for the analysis of biomedical data. These better analyses have enhanced the conclusions that can be drawn from biomedical data, and they have changed the way that experiments are designed and performed. This volume, along with previous and forthcoming 'Computer Methods' volumes for the Methods in Enzymology serial, aims to inform biomedical researchers about recent applications of modern data analysis and simulation methods as applied to biomedical research.

Redox Cell Biology and Genetics, Part A
Redox Cell Biology and Genetics, Part A

Author: Chandan K. Sen

Publisher: Elsevier

Published: 2002-07-22

Total Pages: 617

ISBN-13: 0080496997

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.Key Features* Cellular Responses* Tissues and Organs

The Giant Vesicle Book
The Giant Vesicle Book

Author: Rumiana Dimova

Publisher: CRC Press

Published: 2019-11-19

Total Pages: 652

ISBN-13: 1498752187

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Giant vesicles are widely used as a model membrane system, both for basic biological systems and for their promising applications in the development of smart materials and cell mimetics, as well as in driving new technologies in synthetic biology and for the cosmetics and pharmaceutical industry. The reader is guided to use giant vesicles, from the formation of simple membrane platforms to advanced membrane and cell system models. It also includes fundamentals for understanding lipid or polymer membrane structure, properties and behavior. Every chapter includes ideas for further applications and discussions on the implications of the observed phenomena towards understanding membrane-related processes. The Giant Vesicle Book is meant to be a road companion, a trusted guide for those making their first steps in this field as well as a source of information required by experts. Key Features • A complete summary of the field, covering fundamental concepts, practical methods, core theory, and the most promising applications • A start-up package of theoretical and experimental information for newcomers in the field • Extensive protocols for establishing the required preparations and assays • Tips and instructions for carefully performing and interpreting measurements with giant vesicles or for observing them, including pitfalls • Approaches developed for investigating giant vesicles as well as brief overviews of previous studies implementing the described techniques • Handy tables with data and structures for ready reference