Molecular Biology of the Cell
Author:
Publisher:
Published: 2002
Total Pages: 0
ISBN-13: 9780815332183
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Lipid Domains
Author:
Publisher: Academic Press
Published: 2015-06-08
Total Pages: 393
ISBN-13: 0128033274
DOWNLOAD EBOOKCurrent Topics in Membranes is targeted toward scientists and researchers in biochemistry and molecular and cellular biology, providing the necessary membrane research to assist them in discovering the current state of a particular field and in learning where that field is heading. This volume offers an up to date presentation of current knowledge in the field of Lipid Domains. - Written by leading experts - Contains original material, both textual and illustrative, that should become a very relevant reference material - The material is presented in a very comprehensive manner - Both researchers in the field and general readers should find relevant and up-to-date information
Biochemistry of Lipids, Lipoproteins and Membranes
Author: J.E. Vance
Publisher: Elsevier
Published: 1991-12-17
Total Pages: 619
ISBN-13: 008086080X
DOWNLOAD EBOOKThe second edition of this book on lipids, lipoprotein and membrane biochemistry has two major objectives - to provide anadvanced textbook for students in these areas of biochemistry,and to summarise the field for scientists pursuing research inthese and related fields. Since the first edition of this book was published in 1985 theemphasis on research in the area of lipid and membrane biochemistry has evolved in new directions. Consequently, thesecond edition has been modified to include four chapters on lipoproteins. Moreover, the other chapters have been extensivelyupdated and revised so that additional material covering the areas of cell signalling by lipids, the assembly of lipids andproteins into membranes, and the increasing use of molecular biological techniques for research in the areas of lipid, lipoprotein and membrane biochemistry have been included. Each chapter of the textbook is written by an expert in the field, but the chapters are not simply reviews of current literature. Rather, they are written as current, readable summaries of these areas of research which should be readily understandable to students and researchers who have a basic knowledge of general biochemistry. The authors were selected fortheir abilities both as researchers and as communicators. In addition, the editors have carefully coordinated the chapters sothat there is little overlap, yet extensive cross-referencing among chapters.
The Biophysics of Cell Membranes
Author: Richard M. Epand
Publisher: Springer
Published: 2017-09-25
Total Pages: 224
ISBN-13: 9811062447
DOWNLOAD EBOOKThis volume focuses on the modulation of biological membranes by specific biophysical properties. The readers are introduced to emerging biophysical approaches that mimick specific states (like membrane lipid asymmetry, membrane curvature, lipid flip-flop, lipid phase separation) that are relevant to the functioning of biological membranes. The first chapter describes innovative methods to mimic the prevailing asymmetry in biological membranes by forming asymmetrical membranes made of monolayers with different compositions. One of the chapters illustrates how physical parameters, like curvature and elasticity, can affect and modulate the interactions between lipids and proteins. This volume also describes the sensitivity of certain ion channels to mechanical forces and it presents an analysis of how cell shape is determined by both the cytoskeleton and the lipid domains in the membrane. The last chapter provides evidence that liposomes can be used as a minimal cellular model to reconstitute processes related to the origin of life. Each topic covered in this volume is presented by leading experts in the field who are able to present clear, authoritative and up-to-date reviews. The novelty of the methods proposed and their potential for a deeper molecular description of membrane functioning are particularly relevant experts in the areas of biochemistry, biophysics and cell biology, while also presenting clear and thorough introductions, making the material suitable for students in these fields as well.
Lipid Rafts
Author: Erhard Bieberich
Publisher: Humana
Published: 2021-08-23
Total Pages: 350
ISBN-13: 9781071608166
DOWNLOAD EBOOKThis volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning more about the function of lipid rafts in many areas of cell biology and medicine.
Plant Lipid Signaling Protocols
Author: Teun Munnik
Publisher: Springer Nature
Published: 2013-05-17
Total Pages: 298
ISBN-13: 1627034013
DOWNLOAD EBOOKAs scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.
Methods in Membrane Lipids
Author: Alex M. Dopico
Publisher: Springer Science & Business Media
Published: 2007-08-30
Total Pages: 1265
ISBN-13: 1588296628
DOWNLOAD EBOOKThis book presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. The volume provides a comprehensive set of techniques for studying membrane lipids with a strong biophysical emphasis. It compares the various available techniques including the pros and cons as seen by the experts.
Cellular Domains
Author: Ivan R. Nabi
Publisher: John Wiley & Sons
Published: 2011-07-12
Total Pages: 772
ISBN-13: 1118015746
DOWNLOAD EBOOKCellular domains play vital roles in a wide range of cellular functions. Defining cellular domains and understanding the molecular basis of their formation is essential to the study of cell functionality. This authoritative reference provides the most comprehensive analysis available on cellular domains, with emphasis on the definition and molecular composition of the domain as well as the functional implications of domain organization.
Membrane Organization and Dynamics
Author: Amitabha Chattopadhyay
Publisher: Springer
Published: 2017-12-06
Total Pages: 387
ISBN-13: 3319666010
DOWNLOAD EBOOKThis volume brings together information on membrane organization and dynamics from a variety of spectroscopic, microscopic and simulation approaches, spanning a broad range of time scales. The implication of such dynamic information on membrane function in health and disease is a topic of contemporary interest. The chapters cover various aspects of membrane lipid and protein dynamics, explored using a battery of experimental and theoretical approaches. The synthesis of information and knowledge gained by utilizing multiple approaches will provide the reader with a comprehensive understanding of the underlying membrane dynamics and function, which will help to develop robust dynamic models for the understanding of membrane function in healthy and diseased states. In the last few years, crystal structures of an impressive number of membrane proteins have been reported, thanks to tremendous advances in membrane protein crystallization techniques. Some of these recently solved structures belong to the G protein-coupled receptor (GPCR) family, which are particularly difficult to crystallize due to their intrinsic flexibility. Nonetheless, these static structures do not provide the necessary information to understand the function of membrane proteins in the complex membrane milieu. This volume will address the dynamic nature of membrane proteins within the membrane and will provide the reader with an up-to date overview of the theory and practical approaches that can be used. This volume will be invaluable to researchers working in a wide range of scientific areas, from biochemistry and molecular biology to biophysics and protein science. Students of these fields will also find this volume very useful. This book will also be of great use to those who are interested in the dynamic nature of biological processes.
Optical Microscopy
Author: Brian Herman
Publisher: Elsevier
Published: 2012-12-02
Total Pages: 462
ISBN-13: 0080571395
DOWNLOAD EBOOKOptical Microscopy: Emerging Methods and Applications covers recent technical advances and new approaches to monitoring and altering cell physiology, examining membrane cytoarchitecture, observing multiple cellular activities and intact organ physiology, plus confocal imaging of live cell function, lifetime imaging, and automated clinical imaging cytometry. The book provides the reader with a synopsis of the most recent technical developments in optical microscopy as applied to scientific research. Each chapter introduces new methods by describing how these overcome limitations inherent in previous techniques. Software, hardware, and other equipment concerns are covered. Additionally, the book reviews current applications in order to stimulate future developments in optical microscopy, encouraging novel uses and new technical advances - Caged compounds, fluorescence ratio imaging, and CCD video cameras - Simultaneous multiple detection and real-time fluorescence microscopy - Simultaneous DIC and quantitative LLF video imaging - Total internal reflectance, time-resolved, and automated fluorescence microscopy - Laser-scanning confocal microscopy - Imaging for calcium measurements, membranes, glycoproteins, living cells, and cancer cells
