Trace and Ultratrace Analysis by HPLC

Trace and Ultratrace Analysis by HPLC

Author: Satinder Ahuja

Publisher: Wiley-Interscience

Published: 1992-04-16

Total Pages: 438

ISBN-13:

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High Performance Liquid Chromatography in Biotechnology Edited By William S. Hancock Analytical chemists, biochemists, or chemical engineers will find this up-to-date guide to HPLC’s recent developments essential to enhancing their on-the-job technical expertise. Extensive coverage includes the broad applications of HPLC, ranging from major chromatographic techniques (including reversed phase, ion exchange, affinity and hydrophobic interaction chromatography) to specific separations such as in monoclonal antibody and nucleic acid purification. The book also highlights the techniques required for a quality control program and such advanced technology as mass spectrometry. 1990 (0 471-82584-0) 576 pp. Unified Separation Science J. Calvin Giddings This advanced text/monograph brings together, for the first time in a single volume, the variety of techniques used for chemical separations by outlining their common underlying mechanisms. The mass transport phenomena underlying all separation processes are developed in a simple physicalmathematical form, facilitating analysis of alternative separation techniques and the factors integral to separation power. The first six chapters provide generic background material applicable to a wide range of separation methods, including the theoretical foundations of separations rooted in transport, flow, and equilibrium phenomena. The final five chapters illustrate specific techniques and methods, including electrophoretic and sedimentation techniques, field-flow fractionation, and chromatography. 1991 (0 471-52089-6) 352 pp.


Organic Trace Analysis by Liquid Chromatography

Organic Trace Analysis by Liquid Chromatography

Author: James Lawrence

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 301

ISBN-13: 0323157025

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Organic Trace Analysis by Liquid Chromatography focuses on high-performance liquid chromatography in the field of formulations analysis, which includes quality control of pharmaceutical preparations, pesticide formulations, cosmetics, and food colors. This book explores the application of liquid chromatography to trace analysis. Organized into 10 chapters, this book starts with an overview of the special requirements of liquid chromatography for trace analysis. This text then compares the approach to gas chromatography and formulations analysis, noting the significant difference. Other chapters consider the liquid chromatography equipment and examine the best types and conditions of instrumentation suitable for trace analysis. This book discusses as well the chromatography theory and includes descriptive accounts of the principles of the different forms of chromatography. The final chapter describes the typical approaches that are used for trace analysis. This book is a valuable resource for analysts engaged in the determination of trace organics in many various substrates.


Trace Analysis with Microcolumn Liquid Chromatography

Trace Analysis with Microcolumn Liquid Chromatography

Author: Milos Krejci

Publisher: CRC Press

Published: 2020-07-24

Total Pages: 217

ISBN-13: 1000104761

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This book provides a detailed description of technical elements of a microbore column liquid chromatograph suitable for use in trace analysis. It presents examples of analyses, especially from the spheres of biochemistry, pharmacology, and environmental analytical chemistry.


Advances in Liquid Chromatography

Advances in Liquid Chromatography

Author: Toshihiko Hanai

Publisher: World Scientific

Published: 1996

Total Pages: 366

ISBN-13: 9789810219062

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This book is a contemporary review of selected subjects in liquid chromatography, especially of the technical development, rather than the applications. The subjects are focused in the biomedical and environmental fields. This is also a troubleshooting record. Complex analytical problems such as sensitivity (sensitive detection by chemiluminescence, coulometric detection, laser based detection, necessity of degassing the system for sensitive detection), difficulty (free radical detection by Electron Spin Resonance, Polarimeter for chiral recognition) and reproducibility (packings for chiral separation and stable bonded silica gels) are solved. Theoretically and environmentally important miniaturizations are described. Individual chapters written by specialists provide information beyond what can be found in general textbooks of liquid chromatography.


Ultra Performance Liquid Chromatography Mass Spectrometry

Ultra Performance Liquid Chromatography Mass Spectrometry

Author: Mu Naushad

Publisher: CRC Press

Published: 2014-03-18

Total Pages: 480

ISBN-13: 1466591544

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Due to its high sensitivity and selectivity, liquid chromatography–mass spectrometry (LC–MS) is a powerful technique. It is used for various applications, often involving the detection and identification of chemicals in a complex mixture. Ultra Performance Liquid Chromatography Mass Spectrometry: Evaluation and Applications in Food Analysis presents a unique collection of up-to-date UPLC-MS/MS methods for the separation and quantitative determination of components, contaminants, vitamins, and aroma and flavor compounds in a wide variety of foods and food products. The book begins with an overview of the history, principles, and advancement of chromatography. It discusses the use of UHPLC techniques in food metablomics, approaches for analysis of foodborne carcinogens, and details of UPLC-MS techniques used for the separation and determination of capsaicinoids. Chapters describe the analysis of contaminants in food, including pesticides, aflatoxin, perfluorochemicals, and acrylamide, as well as potentially carcinogenic heterocyclic amines in cooked foods. The book covers food analysis for beneficial compounds, such as the determination of folate, vitamin content analysis, applications for avocado metabolite studies, virgin olive oil component analysis, lactose determination in milk, and analysis of minor components of cocoa and phenolic compounds in fruits and vegetables. With contributions by experts in interdisciplinary fields, this reference offers practical information for readers in research and development, production, and routing analysis of foods and food products.


Ultra-High Performance Liquid Chromatography and Its Applications

Ultra-High Performance Liquid Chromatography and Its Applications

Author: Q. Alan Xu

Publisher: John Wiley & Sons

Published: 2013-04-01

Total Pages: 342

ISBN-13: 1118533984

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Explores both the benefits and limitations of new UHPLC technology High performance liquid chromatography (HPLC) has been widely used in analytical chemistry and biochemistry to separate, identify, and quantify compounds for decades. The science of liquid chromatography, however, was revolutionized a few years ago with the advent of ultra-high performance liquid chromatography (UHPLC), which made it possible for researchers to analyze sample compounds with greater speed, resolution, and sensitivity. Ultra-High Performance Liquid Chromatography and Its Applications enables readers to maximize the performance of UHPLC as well as develop UHPLC methods tailored to their particular research needs. Readers familiar with HPLC methods will learn how to transfer these methods to a UHPLC platform and vice versa. In addition, the book explores a variety of UHPLC applications designed to support research in such fields as pharmaceuticals, food safety, clinical medicine, and environmental science. The book begins with discussions of UHPLC method development and method transfer between HPLC and UHPLC platforms. It then examines practical aspects of UHPLC. Next, the book covers: Coupling UHPLC with mass spectrometry Potential of shell particles in fast liquid chromatography Determination of abused drugs in human biological matrices Analyses of isoflavones and flavonoids Therapeutic protein characterization Analysis of illicit drugs The final chapter of the book explores the use of UHPLC in drug metabolism and pharmacokinetics studies for traditional Chinese medicine. With its frank discussions of UHPLC's benefits and limitations, Ultra-High Performance Liquid Chromatography and Its Applications equips analytical scientists with the skills and knowledge needed to take full advantage of this new separation technology.


Development of a Laser-based Infrared Detector for High Pressure Liquid Chromatography

Development of a Laser-based Infrared Detector for High Pressure Liquid Chromatography

Author: Caroline Stokl

Publisher:

Published: 1987

Total Pages: 394

ISBN-13:

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"An infrared detector is constructed for use in High Pressure Liquid Chromatography. The detector uses a helium-neon laser which emits at 3.39 microns in the infrared as the source and a commercial lead selenide semiconductor as the detector. The detector when used in the flow-injection analysis mode can detect all classes of lipid: fatty acids, triglycerides, phospholipids, cholesterols and cholestryl esters. Chromatography using a normal phase column with separation into the lipid classes of cholesterol, cholestryl esters and triglycerides is performed. A purchased serum sample has been prepared and separation and quantitation of the 3 lipid classes is performed."--