Laboratory Methods in Enzymology: Protein Part D

Laboratory Methods in Enzymology: Protein Part D

Author:

Publisher: Academic Press

Published: 2015-06-18

Total Pages: 161

ISBN-13: 0128003359

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The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 520 volumes and 40,000 chapters in the collection, much of the material is still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. In this volume, number 545, we have brought together a number of core protocols concentrating on protein, carefully written and edited by experts. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - Brings together a number of core protocols concentrating on protein


Laboratory Methods in Enzymology: Protein Part C

Laboratory Methods in Enzymology: Protein Part C

Author:

Publisher: Academic Press

Published: 2014-03-22

Total Pages: 291

ISBN-13: 0124201784

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In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on Protein


Laboratory Methods in Enzymology: Protein Part B

Laboratory Methods in Enzymology: Protein Part B

Author:

Publisher: Academic Press

Published: 2014-02-24

Total Pages: 240

ISBN-13: 0124201792

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Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on protein


Computer Methods Part B

Computer Methods Part B

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Publisher: Academic Press

Published: 2009-12-03

Total Pages: 712

ISBN-13: 9780123750235

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The combination of faster, more advanced computers and more quantitatively oriented biomedical researchers has recently yielded new and more precise methods for the analysis of biomedical data. These better analyses have enhanced the conclusions that can be drawn from biomedical data, and they have changed the way that experiments are designed and performed. This volume, along with previous and forthcoming Computer Methods volumes for the Methods in Enzymology serial, aims to inform biomedical researchers about recent applications of modern data analysis and simulation methods as applied to biomedical research. * Presents step-by-step computer methods and discusses the techniques in detail to enable their implementation in solving a wide range of problems * Informs biomedical researchers of the modern data analysis methods that have developed alongside computer hardware *Presents methods at the "nuts and bolts" level to identify and resolve a problem and analyze what the results mean


Laboratory Methods in Enzymology: Protein Part A

Laboratory Methods in Enzymology: Protein Part A

Author:

Publisher: Academic Press

Published: 2014-01-08

Total Pages: 197

ISBN-13: 0124200974

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The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including: - Pulse-chase analysis to measure protein degradation - Labeling a protein with fluorophores using NHS ester derivitization - Immunoaffinity purification of proteins - Proteolytic affinity tag cleavage - Purification of GST-tagged proteins - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - This volume focuses on core protocols involving protein


Laboratory Methods in Enzymology: DNA

Laboratory Methods in Enzymology: DNA

Author:

Publisher: Elsevier

Published: 2013-09-02

Total Pages: 405

ISBN-13: 0124199542

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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on DNA


Laboratory Methods in Enzymology: RNA

Laboratory Methods in Enzymology: RNA

Author: Jon Lorsch

Publisher: Academic Press

Published: 2013-09-11

Total Pages: 472

ISBN-13: 0124200516

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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on RNA


Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate

Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate

Author:

Publisher: Academic Press

Published: 2013-10-30

Total Pages: 408

ISBN-13: 012420094X

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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate


Guide to Yeast Genetics and Molecular and Cell Biology, Part C

Guide to Yeast Genetics and Molecular and Cell Biology, Part C

Author:

Publisher: Academic Press

Published: 2002-06-14

Total Pages: 735

ISBN-13: 9780121822545

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This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include cytology, biochemistry, cell fractionation, and cell biology.


Recombinant Protein Expression: Eukaryotic hosts

Recombinant Protein Expression: Eukaryotic hosts

Author:

Publisher: Academic Press

Published: 2021-11-04

Total Pages: 384

ISBN-13: 0323907385

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Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more. Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambrĀ® 250, and Expression of recombinant antibodies in Leishmania tarentolae. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology serial Updated release includes the latest information on Recombinant Protein Expression