In Situ Hybridization Protocols
In Situ Hybridization Protocols

Author: Boye Nielsen

Publisher: Humana Press

Published: 2014-09-13

Total Pages: 0

ISBN-13: 9781493914586

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In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.

In Situ Hybridization Protocols
In Situ Hybridization Protocols

Author: Ian A. Darby

Publisher: Taylor & Francis US

Published: 2000

Total Pages: 368

ISBN-13: 9780896036864

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Annotation Darby (human biology, RMIT U., Victoria, Australia) is joined by geneticists, molecular biologists, and pathologists from around the world to describe basic and advanced techniques for hybridization, for whole-mount embryo specimens and at the electron microscope level. Coverage includes protocols for detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA and RNA probes to detect expression in cells or tissue sections. For novice and experienced investigators who need proven and readily reproducible methods. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Fluorescence In Situ Hybridization (FISH) - Application Guide
Fluorescence In Situ Hybridization (FISH) - Application Guide

Author: Thomas Liehr

Publisher: Springer Science & Business Media

Published: 2008-11-26

Total Pages: 447

ISBN-13: 3540705813

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This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.

In Situ Hybridization Methods
In Situ Hybridization Methods

Author: Giselbert Hauptmann

Publisher: Humana

Published: 2016-10-05

Total Pages: 0

ISBN-13: 9781493944637

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This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.

Basic and Advanced Laboratory Techniques in Histopathology and Cytology
Basic and Advanced Laboratory Techniques in Histopathology and Cytology

Author: Pranab Dey

Publisher: Springer

Published: 2018-06-08

Total Pages: 275

ISBN-13: 9811082529

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This book provides detailed information on basic and advanced laboratory techniques in histopathology and cytology. It discusses the principles of and offers clear guidance on all routine and special laboratory techniques. In addition, it covers various advanced laboratory techniques, such as immunocytochemistry, flow cytometry, liquid based cytology, polymerase chain reaction, tissue microarray, and molecular technology. Further, the book includes numerous color illustrations, tables and boxes to familiarize the reader with the work of a pathology laboratory. The book is mainly intended for postgraduate students and fellows in pathology as well as practicing pathologists. The book is also relevant for all the laboratory technicians and students of laboratory technology.

PCR in Situ Hybridization
PCR in Situ Hybridization

Author: Gerard J. Nuovo

Publisher: Lippincott Williams & Wilkins

Published: 1997

Total Pages: 536

ISBN-13:

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Describes the technique whereby the extreme sensitivity of the polymerase chain reaction (PCR) is combined with the cell localizing ability of in situ hybridization. This revised and updated edition contains chapters on the basics of molecular biology; the nonspecific pathways of PCR; applications of PCR in situ hybridization--human papillomavirus, and HIV-1; and instrumentation. There is also an appendix on reagents for molecular biological analyses. Annotation copyright by Book News, Inc., Portland, OR

Practical in Situ Hybridization
Practical in Situ Hybridization

Author: Trude Schwarzacher

Publisher: Garland Science

Published: 2000

Total Pages: 203

ISBN-13: 9781859961384

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Practical in situ Hybridization is aimed at those who wish to learn and use efficient and reliable protocols in their work, and at researchers checking the validity and interpretation of published data.; In situ hybridization methods can be used to identi

Modern Techniques in Cytopathology
Modern Techniques in Cytopathology

Author: M.M. Bui

Publisher: Karger Medical and Scientific Publishers

Published: 2020-01-13

Total Pages: 120

ISBN-13: 3318065765

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In the era of precision medicine, physicians are increasingly in need of more definitive diagnostic, prognostic, and predictive information derived from small biopsy specimens such as cytology samples in order to guide effective patient care. Cytopathology is well poised to meet this challenge. Whilst the traditional cytomorphologic component of cytology practice is still valid, enormous advances have been made in the field of cytopathology thanks to transformative technology and innovative individuals that have augmented the cytologists' ability to meet the demands of modern medicine. The purpose of this book is to describe, illustrate, and review many of the most recent developments regarding modern techniques employed in cytopathology. This latest monograph is intended for all cytologists including cytopathologists, cytotechnologists, cytology lab assistants, trainees, research scientists, and anyone who is interested in the field of cytopathology. We have invited pioneering experts in their respective fields to author these chapters. This book is not only the culmination of their groundbreaking work and effort but also presents a critical review of the current literature. We have attempted to provide readers with an informative and comprehensive aid so that they may better appreciate how emerging technology has been applied to cytology. Each chapter in this book presents a stand-alone contemporary review of emerging topics in cytopathology. We hope that you will find this monograph thought-provoking and a valuable reference for your practice.

In Situ Hybridization in Electron Microscopy
In Situ Hybridization in Electron Microscopy

Author: Gerard Morel

Publisher: CRC Press

Published: 2001-01-30

Total Pages: 634

ISBN-13: 1000611396

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In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization