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Author: John Goers
Publisher:
Published: 1993
Total Pages: 212
ISBN-13:
DOWNLOAD EBOOKThis is a laboratory manual for a course in immunochemical techniques designed to introduce students to the subject, enabling them to understand the wider applications of the techniques they are using. The text is written with easy-to-follow protocols for immunological techniques used in modern research labs and biochemical companies.
Author: Jørgen Clausen
Publisher: Elsevier Science Limited
Published: 1988
Total Pages: 464
ISBN-13: 9780444809094
DOWNLOAD EBOOKRapid expansion in the field of immunochemistry has necessitated the publication of a new edition to succeed the indispensable present manual. As its predecessor, this revised edition presents you with the data and practical advice you need on the application of the rapidly expanding mass of data on immunochemistry. More specifically, the book examines the possibilities of employing immunological microtechniques in biochemical and histochemical analyses. The reader will find up-to-date information on the practical application of immunochemical analysis for laboratory use. Immunochemical Techniques especially stresses the possible errors and misinterpretations which may occur. Revised and extended chapters include: lectins, HLA-antigens, and crossed-electrophoresis. New chapters deal with: the complement system, enzyme immunoassay systems, immuno-fluorescence methods, immunoadsorbent techniques, and recent developments in methodology. Conveniently available in hardcover and paperback, this volume is unsurpassed both as a practical reference work, and as a laboratory manual.
Author: A. Nowotny
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 206
ISBN-13: 3662001489
DOWNLOAD EBOOKThis book intends to be neither a complete survey of the field nor an exhaustive source of references. For these purposes, the use of the extensive compilation "Experimental Immunochemistry" by E. A. KABAT and M. M. MAYER (1962) or the excellent methodological textbook, "Methods in Immunology", by D. H. CAMPBELL, J. S. GARVEY, E. E. CREMER and D. H. SUSSDORF (1963), or the quite comprehensive series "Methods in Immunology and Immunochemistry" by C. A. WILLIAMS and M. W. CHASE (1967) are more suitable. The main purpose of this manual ist to provide students with a simple book which will introduce them to some frequently occurring problems in the three major sections of the immunochemistry of natural products. These are the isolation of the materials, the chemical analysis of the constituents and their structure, and, finally, the assays of the most important biological and immunological activities. In this manual the exercises are simplified and several shortcuts are taken in order to fit them into the framework of a teaching course. The introduction to each exercise gives a brief and elementary explanation of the reaction on which it is based. "Materials and Equipment" lists all tissues or cells, chemicals, glassware, and special equipment which must be available to carry out the exercise, although the very common laboratory tools are usually omitted from the list.
Author: John Pound
Publisher: Humana Press
Published: 1998-04-02
Total Pages: 508
ISBN-13: 9780896033887
DOWNLOAD EBOOKThis much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins, and their various conjugates. The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities.
Author: John Pound
Publisher:
Published: 1998
Total Pages:
ISBN-13:
DOWNLOAD EBOOKThis much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including conventional" and recombinant antibodies, fusion proteins, and their various conjugates. The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities."
Author: Heddy Zola
Publisher: CRC Press
Published: 1990-05-23
Total Pages: 288
ISBN-13:
DOWNLOAD EBOOKThis two-volume reference details immunological techniques for biologists of all disciplines. Volume I includes a detailed discussion of the tissue culture laboratory. It addresses what the lab needs to be, and the general "housekeeping" procedures involved in tissue culture. Presented next are chapters on specific aspects of tissue culture and hybridoma technology. The book includes a review of bioassays for interleukins, and a series of papers on lymphokines and functional assays in vitro. The section on molecular genetic studies begins with consideration of the choice of strategies for cloning the genes of cell surface molecules. It continues with papers on aspects of molecular biological techniques most closely related to immunology. The final section covers immunochemical techniques. Volume II reviews techniques used with small laboratory animals. It includes papers on specialized procedures with animals. Technical aspects are emphasized through a detailed analysis of effects of ultraviolet light on the immune system. Covered also is antigen detection in cells and tissue. The book addresses the important areas of protein purification using monoclonal antibodies.These two volumes are of great importance to those who use immunological techniques, whether they are immunologists or trained in other disciplines. The book is intended for those in animal science, veterinary science, genetics and cell biology, bacteriology, immunology, pathology, biochemistry, laboratory medicine, and hematology.
Author: Laura Caponi
Publisher: Springer Science & Business Media
Published: 2013-03-09
Total Pages: 117
ISBN-13: 3662039427
DOWNLOAD EBOOKA handy lab manual that allows quick and easy access to the techniques commonly used in analysing antibody specificity. It describes some of the most useful immunological techniques based on antibodies, including ELISA, immunoblotting and immunoprecipitation protocols that provide useful tools for recognising immunological specificities, together with basic immunofluorescence and immunohistochemistry procedures for the in situ identification of antigens. The topics are discussed from a practical point of view, combining the theoretical basis of each technique with sample protocols and a troubleshooting guide. Attention is focused on the various aspects of the protocols described thus providing readers with the maximum possible information on each technique. XXXXXXX NEUER TEXT This handy lab manual permits quick access to the techniques commonly used to analyze antibody specificity. The most useful immunological techniques are described, providing readers with practical tools for recognizing immunological specificities and procedures for the in situ identification of antigens. The theoretical basis of each technique is described and sample protocols and troubleshooting tips are included. A Springer Lab Manual
Author: Edward Harlow
Publisher: CSHL Press
Published: 1999
Total Pages: 524
ISBN-13: 9780879695446
DOWNLOAD EBOOKAuthor: Trung Nguyen
Publisher: Cambridge University Press
Published: 2022-07-07
Total Pages: 283
ISBN-13: 1009116053
DOWNLOAD EBOOKThis book will enable practitioners to understand the many complex intricacies of immunohistochemistry (IHC) and make best use of this powerful analytical tool. Providing a thorough grounding in the fundamentals of immunohistochemistry, the book includes several chapters on robotics and automation technology, giving key information on the design of machines and tips to maximise workflow efficiencies. The relationship between IHC and molecular pathology is explained clearly, demonstrating the increasing impact on personalized medicine and targeted therapies for cancer patients. The staining protocol is deconstructed, allowing the reader to adapt it for a variety of diagnostic and research applications. Written by experts at the forefront of hospital immunohistochemistry, there is a strong emphasis on practical guidance on a range of techniques as well as troubleshooting of common problems driven by the authors' experiences. Extensively illustrated with high-quality colour images, this is an invaluable resource to all pathology practitioners utilising the technique.
Author: Alois Nowotny
Publisher:
Published: 1969
Total Pages: 197
ISBN-13:
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