Immunoassay Automation: A Practical Guide describes automation of immunoassay from the practical viewpoint of the clinical laboratory. General introduction and evaluation sections demonstrate principles and practice. A comprehensive selection of available systems are detailed by experts, with a view towards popularity, technical advances, and operational efficiency. This laboratory guide is essential for practitioners in clinical chemistry laboratories, and will have lasting value in the evolution of automated systems.Focuses on automation of immunoassay for the clinical laboratoryEmphasizes principles, method evaluation, and the systems approachAids system selection by evaluation of technical, clinical, operational, and economical parametersContains complete descriptions by experts on the latest automated immunoassay systemsBased upon the editor's well-received workshops on automated immunoassay
Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians. - Presents up-to-date information - Provides extensive cross-referencing - Covers theory and practice in full detail - Written by leading authorities
Many new systems have developed since the publication of Immunoassay Automation: A Practical Guide in 1992. Dr. Chan's updated guide is a supplement to his earlier volume, not a replacement of it. He discussesthe changing clinical laboratory environment and summarizes automated immunoassay systems. He then goes on to describe each system in-depth, including an introduction, a description of the instrumentation, the reagent, and the performance system. Provides a general discussion of the changing environment of testing in the clinical laboratory Offers a summary of automated immunoassay systems Serves as a practical guide to using the following systems: AxSym, Opus Magnum, VIDAS, Radius, ACS 180, Immulite, ACA Plus, Immuno 1, Coas CORE, Access, AIA 1200DX, AutoDELFIA, O1B, Copalis, and Universal solid phase microtiter plate system.
Containing updated and new information on advanced technology - including micro and nanoscale immunoassays - this text provides a mix of practical information coupled with a review of clincal applications and practical examples.
The perfect balance of theory and practice! Here’s the practical introduction you need to understand the essential theoretical principles of clinical immunology and the serological and molecular techniques commonly used in the laboratory. You’ll begin with an introduction to the immune system; then explore basic immunologic procedures; examine immune disorders; and study the serological and molecular diagnosis of infectious disease. An easy-to-read, student-friendly approach emphasizes the direct application of theory to clinical laboratory practice. Each chapter is a complete learning module with learning outcomes, chapter outlines, theoretical principles, illustrations, and definitions of relevant terminology. Review questions and case studies help you assess your mastery of the material. A glossary at the end of the book puts must-know information at your fingertips. An access code inside new printed texts unlocks Lab Exercises and Branching Case Studies online at FADavis.com that offer more opportunities to apply theory to clinical laboratory practice.
Each chapter of this book aims to explore the basic physical and chemical principles involved in the immunoassay techniques discussed. The book also looks at the optimization and limitations of methodology and concludes with a brief overview of the application of the performance of the technology.
Using a discipline-by-discipline approach, Linne & Ringsrud's Clinical Laboratory Science: Concepts, Procedures, and Clinical Applications, 7th Edition provides a fundamental overview of the skills and techniques you need to work in a clinical laboratory and perform routine clinical lab tests. Coverage of basic laboratory techniques includes key topics such as safety, measurement techniques, and quality assessment. Clear, straightforward instructions simplify lab procedures, and are described in the CLSI (Clinical and Laboratory Standards Institute) format. Written by well-known CLS educator Mary Louise Turgeon, this text includes perforated pages so you can easily detach procedure sheets and use them as a reference in the lab! Hands-on procedures guide you through the exact steps you'll perform in the lab. Review questions at the end of each chapter help you assess your understanding and identify areas requiring additional study. A broad scope makes this text an ideal introduction to clinical laboratory science at various levels, including CLS/MT, CLT/MLT, and Medical Assisting, and reflects the taxonomy levels of the CLS/MT and CLT/MLT exams. Detailed full-color illustrations show what you will see under the microscope. An Evolve companion website provides convenient online access to all of the procedures in the text, a glossary, audio glossary, and links to additional information. Case studies include critical thinking and multiple-choice questions, providing the opportunity to apply content to real-life scenarios. Learning objectives help you study more effectively and provide measurable outcomes to achieve by completing the material. Streamlined approach makes it easier to learn the most essential information on individual disciplines in clinical lab science. Experienced author, speaker, and educator Mary Lou Turgeon is well known for providing insight into the rapidly changing field of clinical laboratory science. Convenient glossary makes it easy to look up definitions without having to search through each chapter. NEW! Procedure worksheets have been added to most chapters; perforated pages make it easy for students to remove for use in the lab and for assignment of review questions as homework. NEW! Instrumentation updates show new technology being used in the lab. NEW! Additional key terms in each chapter cover need-to-know terminology. NEW! Additional tables and figures in each chapter clarify clinical lab science concepts.
Immunodiagnostic tests are analytical methods that use antibodies as reagents whose results are used to aid diagnosis and are widely used in many scientific disciplines and in many different ways. Perhaps the most widespread and obvious use is in clinical applications, but immunodiagnostic tests are also used in other fields such as forensic science and environmental and food analysis. The different types of test range from simple manual methods to fully automated systems with sophisticated integrated detection. Immunodiagnostics: A Practical Approach starts off by explaining the principles and development of immunodiagnostic tests, specifically the use of radioisotopes as tracers. Chapter 2 explains the use of solid-phase supports to bind immunoreagents. Enzymes are widely used as labels in immunoassays and their use with colourimetric, fluorimetric, and chemiluminescent detection systems is described. The use of enzymes as labels reflects the move away from radioisotopes and one of the most powerful non- radioisotpoc prodcedures is the time-resolved fluorescence assay. Enzymes can also be used as a simple method of obtaining high performance from immunodiagnostics and this application is covered later in the book. The next set of techniques to be described are light scattering techniques, which can be used in either simple manual assays or in sophisticated automated procedures. The penultimate chapter describes the principles of automation of immunodiagnostic tests. The last topic to be discussed is that of quality assurance.
A very broad range of professionals are using immunoassay technology daily to analyze genetically engineered (GE) crops and related areas, and many of these professionals are completely new to this technology. There is a great need for users to have a book containing technical and practical guidance, and describing limitations and pitfalls of applying immunoassay in agricultural biotechnology. This book focuses on the application of immunoassays to GE plants and related areas. A group of international experts from government agencies, academics and industries, who have many years of related experience, contribute high quality chapters in their areas of expertise. This book covers topics including principles of immunoassay, antibody engineering in AgBiotech, current technologies (formats, kit development, manufacturing and quality control), method validation, applications in trait discovery and product development, applications in grain products and food processing, applications in environmental monitoring, automation and high throughput, reference materials, data interpretation and source of error, and future perspectives and challenges. In addition, to meet the practical needs for a variety of readers from different backgrounds, methods and protocols are included as well.
The 5th edition of this classic text sets the standard for comprehensive coverage of immunology. Building from a solid foundation of knowledge and skills, trusted author Mary Louise Turgeon takes you from basic immunologic mechanisms and serologic concepts to the theory behind the procedures you’ll perform in the lab. Immunology & Serology in Laboratory Medicine, Fifth Edition is the go-to resource for everything from mastering automated techniques to understanding immunoassay instrumentation and disorders of infectious and immunologic origin. Packed with learning objectives, review questions, step-by-step procedures, and case studies, this text is your key to succeeding in today’s modern laboratory environment. Full-color, six-page insert of photomicrographs provide a better picture of what you’ll see in the laboratory. Learning objectives at the beginning of each chapter offer a measurable outcome you can achieve by completing the material. Chapter highlights at the end of each chapter provide a summary of the most important information covered in each chapter. Review questions at the end of each chapter are tied to learning objectives further enhance your understanding. Case studies challenge you to apply your knowledge and help strengthen your critical thinking skills. Glossary at the end of the book provides quick access to key terms and definitions. NEW! Expanded chapter on Vaccines as the importance of vaccines continues to become more evident. NEW! Updated chapter on Molecular Techniques incorporates the newest technology specific to immunology. NEW! Key terms at the beginning of each chapter help you learn the important vocabulary in immunology. NEW! Case studies with added multiple-choice questions in addition to critical thinking questions will help you apply your knowledge and develop critical-thinking skills.
