Handbook of RNA Biochemistry
Handbook of RNA Biochemistry

Author: Roland K. Hartmann

Publisher: John Wiley & Sons

Published: 2015-06-22

Total Pages: 1370

ISBN-13: 3527327762

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The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA.

RNA-Protein Complexes and Interactions
RNA-Protein Complexes and Interactions

Author: Ren-Jang Lin

Publisher: Springer Nature

Published: 2023-05-11

Total Pages: 349

ISBN-13: 1071631918

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This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA-Protein Complexes and Interactions: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.

Handbook of Nucleic Acid Purification
Handbook of Nucleic Acid Purification

Author: Dongyou Liu

Publisher: CRC Press

Published: 2009-01-14

Total Pages: 572

ISBN-13: 1420070975

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An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic a

RNA Recognition
RNA Recognition

Author:

Publisher: Academic Press

Published: 2019-06-23

Total Pages: 470

ISBN-13: 0128168323

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RNA Recognition, Volume 623, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This updated volume covers a variety of topics, including The Preparation of cooperative RNA recognition complexes for crystallographic structural studies, Methods for thermal denaturation studies of fluorogenic aptamers, Dynamic combinatorial chemistry as a rapid, fragment-based approach to RNA-targeted compound discovery, Using a click chemistry assay to identify natural product ligands for pre-microRNAs, Lessons from exploration of chemical and structural small molecule:RNA space, Using ligand-observed NMR to study RNA-small molecule interactions, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on RNA Recognition

Biocatalysts and Enzyme Technology
Biocatalysts and Enzyme Technology

Author: Klaus Buchholz

Publisher: John Wiley & Sons

Published: 2012-12-21

Total Pages: 609

ISBN-13: 3527672001

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This second edition of a bestselling textbook offers an instructive and comprehensive overview of our current knowledge of biocatalysis and enzyme technology. The book now contains about 40% more printed content. Three chapters are completely new, while the others have been thoroughly updated, and a section with problems and solutions as well as new case studies have been added. Following an introduction to the history of enzyme applications, the text goes on to cover in depth enzyme mechanisms and kinetics, production, recovery, characterization and design by protein engineering. The authors treat a broad range of applications of soluble and immobilized biocatalysts, including wholecell systems, the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering. Methods to estimate the sustainability, important internet resources and their evaluation, and legislation concerning the use of biocatalysts are also covered.

Modified Nucleic Acids
Modified Nucleic Acids

Author: Kazuhiko Nakatani

Publisher: Springer

Published: 2016-04-04

Total Pages: 280

ISBN-13: 3319271113

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This book spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-MW ligands to nucleic acids and the design of unnatural base pairs. This unique book addresses new developments and is designed for graduate level and professional research purposes.

Biochemistry Laboratory Manual For Undergraduates
Biochemistry Laboratory Manual For Undergraduates

Author: Timea Gerczei Fernandez

Publisher: Walter de Gruyter GmbH & Co KG

Published: 2015-03-11

Total Pages: 190

ISBN-13: 3110426250

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Biochemistry laboratory manual for undergraduates – an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.

RNA Methodologies
RNA Methodologies

Author: Robert E. Farrell Jr.

Publisher: Academic Press

Published: 2017-08-11

Total Pages: 877

ISBN-13: 0128046791

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RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise. - Features new material in miRNA, MIQE guidelines, biomarkers, RNA sequencing, digital PCR and more - Includes expanded coverage on quantitative PCR techniques, RNAi, bioinformatics, the role of locked nucleic acids, aptamer biology, PCR arrays, and other modern technologies - Presents comprehensive, cutting-edge information covering all aspects of working with RNA - Builds from basic information on RNA techniques to in-depth protocols to guidance on how to modify and adjust each step of a particular application - Presents multiple avenues for addressing the same experimental goals