Grab, Manipulate and Watch Single DNA Molecule Replication
Grab, Manipulate and Watch Single DNA Molecule Replication

Author:

Publisher:

Published: 2023

Total Pages: 0

ISBN-13: 9789464199550

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DNA replication is a critical process intrinsic to the sustenance and propagation of life, involving a symphony of enzymes including helicases, polymerases, and single-stranded DNA binding proteins (SSBs). These enzymes collaboratively ensure the precise duplication of genetic information, a process that, despite significant research, has elements yet to be fully elucidated, especially intermediary steps and the dynamic engagements between replicative proteins. Single-molecule techniques have recently blossomed, offering enhanced insights into the real-time dynamics and interactions of individual molecules in their natural settings, unveiling obscured intermediate steps and enzyme conformational changes. Chapter 1 outlines the thesis's primary aim, elucidating DNA replication mechanisms. Starting with fundamental biological notions, this chapter transitions to discuss the non-equilibrium nature of living systems, emphasizing the role of single-molecule investigations. Such studies have enhanced our understanding of non-equilibrium systems, revealing cellular mechanisms and influencing factors. SSBs are crucial for maintaining genome integrity as they bind to ssDNA and coordinate with various proteins involved in DNA replication, recombination, and repair. Chapter 2 offers a comprehensive overview of recent advances in our understanding of SSBs, as elucidated by single-molecule assays such as optical tweezers, magnetic tweezers, Förster resonance energy transfer, and their combinations. These techniques have provided novel insights into the dynamics of SSB binding to ssDNA and its interactions with other proteins, emphasizing the central role of SSB in modulating the activities of other proteins. Chapter 3 presents the single-molecule observations of the T7 bacteriophage single-stranded DNA-binding protein (gp2.5) binding to ssDNA. Our experiments demonstrate the significant influence of the base sequence, ssDNA conformation, and the acidic terminal domain of T7 gp2.

The Double Helix
The Double Helix

Author: James D. Watson

Publisher: Signet Book

Published: 1969-02

Total Pages: 143

ISBN-13: 9780451037701

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Since its publication in 1968, The Double Helix has given countless readers a rare and exciting look at one highly significant piece of scientific research-Watson and Crick's race to discover the molecular structure of DNA.

Structural DNA Nanotechnology
Structural DNA Nanotechnology

Author: Nadrian C. Seeman

Publisher: Cambridge University Press

Published: 2015

Total Pages: 269

ISBN-13: 0521764483

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Written by the founder of the field, this is a comprehensive and accessible introduction to structural DNA nanotechnology.

Concepts of Biology
Concepts of Biology

Author: Samantha Fowler

Publisher:

Published: 2023-05-12

Total Pages: 0

ISBN-13: 9781739015503

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Black & white print. Concepts of Biology is designed for the typical introductory biology course for nonmajors, covering standard scope and sequence requirements. The text includes interesting applications and conveys the major themes of biology, with content that is meaningful and easy to understand. The book is designed to demonstrate biology concepts and to promote scientific literacy.

Life's Ratchet
Life's Ratchet

Author: Peter M. Hoffmann

Publisher:

Published: 2012-10-30

Total Pages: 290

ISBN-13: 0465022537

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Life, Hoffman argues, emerges from the random motions of atoms filtered through the sophisticated structures of our evolved machinery. People are essentially giant assemblies of interacting nanoscale machines.

Beginning Perl for Bioinformatics
Beginning Perl for Bioinformatics

Author: James Tisdall

Publisher: "O'Reilly Media, Inc."

Published: 2001-10-22

Total Pages: 388

ISBN-13: 0596550472

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With its highly developed capacity to detect patterns in data, Perl has become one of the most popular languages for biological data analysis. But if you're a biologist with little or no programming experience, starting out in Perl can be a challenge. Many biologists have a difficult time learning how to apply the language to bioinformatics. The most popular Perl programming books are often too theoretical and too focused on computer science for a non-programming biologist who needs to solve very specific problems.Beginning Perl for Bioinformatics is designed to get you quickly over the Perl language barrier by approaching programming as an important new laboratory skill, revealing Perl programs and techniques that are immediately useful in the lab. Each chapter focuses on solving a particular bioinformatics problem or class of problems, starting with the simplest and increasing in complexity as the book progresses. Each chapter includes programming exercises and teaches bioinformatics by showing and modifying programs that deal with various kinds of practical biological problems. By the end of the book you'll have a solid understanding of Perl basics, a collection of programs for such tasks as parsing BLAST and GenBank, and the skills to take on more advanced bioinformatics programming. Some of the later chapters focus in greater detail on specific bioinformatics topics. This book is suitable for use as a classroom textbook, for self-study, and as a reference.The book covers: Programming basics and working with DNA sequences and strings Debugging your code Simulating gene mutations using random number generators Regular expressions and finding motifs in data Arrays, hashes, and relational databases Regular expressions and restriction maps Using Perl to parse PDB records, annotations in GenBank, and BLAST output

The Science and Applications of Synthetic and Systems Biology
The Science and Applications of Synthetic and Systems Biology

Author: Institute of Medicine

Publisher: National Academies Press

Published: 2011-12-30

Total Pages: 570

ISBN-13: 0309219396

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Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.

Single-molecule Techniques
Single-molecule Techniques

Author: Paul R. Selvin

Publisher: CSHL Press

Published: 2008

Total Pages: 511

ISBN-13: 087969775X

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Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.

K-12 Education: Concepts, Methodologies, Tools, and Applications
K-12 Education: Concepts, Methodologies, Tools, and Applications

Author: Management Association, Information Resources

Publisher: IGI Global

Published: 2013-09-30

Total Pages: 1765

ISBN-13: 1466645032

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Primary and Secondary education is a formative time for young students. Lessons learned before the rigors of higher education help to inform learners’ future successes, and the increasing prevalence of learning tools and technologies can both help and hinder students in their endeavors. K-12 Education: Concepts, Methodologies, Tools, and Applications investigates the latest advances in online and mobile learning, as well as pedagogies and ontologies influenced by current developments in information and communication technologies, enabling teachers, students, and administrators to make the most of their educational experience. This multivolume work presents all stakeholders in K-12 education with the tools necessary to facilitate the next generation of student-teacher interaction.

Introducing Epigenetics
Introducing Epigenetics

Author: Cath Ennis

Publisher: Icon Books Ltd

Published: 2017-02-02

Total Pages: 285

ISBN-13: 1848319037

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Epigenetics is the most exciting field in biology today, developing our understanding of how and why we inherit certain traits, develop diseases and age, and evolve as a species. This non-fiction comic book introduces us to genetics, cell biology and the fascinating science of epigenetics, which is rapidly filling in the gaps in our knowledge, allowing us to make huge advances in medicine. We'll look at what identical twins can teach us about the epigenetic effects of our environment and experiences, why certain genes are 'switched on' or off at various stages of embryonic development, and how scientists have reversed the specialization of cells to clone frogs from a single gut cell. In Introducing Epigenetics, Cath Ennis and Oliver Pugh pull apart the double helix, examining how the epigenetic building blocks and messengers that interpret and edit our genes help to make us, well, us.